A5074145664- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Gout, Hyperuricemia, Uric Acid
- Neuroscience and Neuropharmacology Research
- Electrolyte and hormonal disorders
- Case Reports on Hematomas
- Potassium and Related Disorders
- Renal function and acid-base balance
- Alcohol Consumption and Health Effects
- Magnesium in Health and Disease
- Drug Transport and Resistance Mechanisms
- Metabolism and Genetic Disorders
- Birth, Development, and Health
- Amino Acid Enzymes and Metabolism
- Pancreatic function and diabetes
- Urticaria and Related Conditions
- Parathyroid Disorders and Treatments
- Liver Disease Diagnosis and Treatment
- Molecular Sensors and Ion Detection
- Biomedical Research and Pathophysiology
- Inflammasome and immune disorders
- Eosinophilic Disorders and Syndromes
- Ion Channels and Receptors
- Dialysis and Renal Disease Management
- Muscle and Compartmental Disorders
Brigham and Women's Hospital
2016-2025
Harvard University
2016-2025
VA Boston Healthcare System
2014-2025
Yale University
2020
University of Shizuoka
2014
Harvard University Press
2005-2012
Institute of Organic Chemistry
2012
Eötvös Loránd University
2012
Pediatric Nephrology of Alabama
2012
National Cheng Kung University
2009
To provide guidance for the management of gout, including indications and optimal use urate-lowering therapy (ULT), treatment gout flares, lifestyle other medication recommendations.Fifty-seven population, intervention, comparator, outcomes questions were developed, followed by a systematic literature review, network meta-analyses with ratings available evidence according to Grading Recommendations Assessment, Development Evaluation (GRADE) methodology, patient input. A group consensus...
Objective To provide guidance for the management of gout, including indications and optimal use urate‐lowering therapy ( ULT ), treatment gout flares, lifestyle other medication recommendations. Methods Fifty‐seven population, intervention, comparator, outcomes questions were developed, followed by a systematic literature review, network meta‐analyses with ratings available evidence according to Grading Recommendations Assessment, Development Evaluation GRADE ) methodology, patient input. A...
Four genes encode electroneutral, Na+-independent, K-Cl cotransporters. KCC2, is exclusively expressed in neurons where it thought to drive intracellular Cl- low concentrations and shift the reversal potential for conductances such as GABA(A) or glycine receptor channels, thus participating postnatal development of inhibitory mechanisms brain. Indeed, expression cotransporter at birth increases postnatally, a time when concentration decreases gamma-aminobutyric acid switches its effect from...
The K+-Cl- cotransporters (KCCs) belong to the gene family of electroneutral cation-chloride cotransporters, which also includes two bumetanide-sensitive Na+-K+-2Cl- and a thiazide-sensitive Na+-Cl- cotransporter. We have cloned cDNAs encoding mouse KCC3, human KCC4, three new members this family. KCC3 KCC4 predict proteins 1083 1150 amino acids, respectively. are 65-71% identical previously characterized transporters KCC1 KCC2, with they share predicted membrane topology. four KCC differ at...
We report the molecular and functional characterization of murine Slc26a6, putative apical chloride-formate exchanger proximal tubule. The Slc26a6 transcript is expressed in several tissues, including kidney. Alternative splicing second exon generates two distinct isoforms, denoted Slc26a6a Slc26a6b, which differ inclusion a 23-residue NH 2 -terminal extension. Functional comparison with Slc26a1, basolateral oxalate tubule, reveals number intriguing differences. Whereas capable Cl − ,...
Rare loss-of-function mutations in the calcium-sensing receptor (Casr) gene lead to decreased urinary calcium excretion context of parathyroid hormone (PTH)-dependent hypercalcemia, but role Casr kidney is unknown. Using animals expressing Cre recombinase driven by Six2 promoter, we generated mice that appeared grossly normal had undetectable levels mRNA and protein kidney. Baseline serum calcium, phosphorus, magnesium, PTH were similar control mice. When challenged with dietary...
We previously observed associations between a Dietary Approaches to Stop Hypertension (DASH)-style diet and large reductions in kidney stone risk. This study examined DASH-style 24-hour excretions of urinary lithogenic factors.We studied 3426 participants with without nephrolithiasis the Health Professionals Follow-up Study (HPFS) Nurses' Studies (NHS) I II. A dietary DASH score was based on seven components: high intake fruits, vegetables, nuts legumes, dairy products, whole grains low...
Gout is a major health problem in the United States; it affects 8.3 million people, which approximately 4% of adult population. most often diagnosed and managed primary care physician practices. Primary physicians have significant opportunity to diagnose manage patients with gout improve patient outcomes. Following publication 2006 European League Against Rheumatism (EULAR) guidelines, evidence on has accumulated new treatments for become available. It objective these 2011 recommendations...
We investigated the novel role of HCA2 (GPR109A) and its ligand nicotinic acid in regulating macrophage function. Hca2 expression RAW264.7 murine cell line is strongly induced by LPS treatment correlates with TNF-α. Treatment 300 μM (reported EC50 3 μM, peak plasma concentration 50–300 μM), significantly inhibited TNF-α, IL-6, IL-12p40, IL-1β production (P<0.05) (1 ng/ml)-stimulated wild-type bone marrow-derived macrophages (BMMs) but failed to do so Hca2–/– BMMs. reduced nuclear factor κB...
Tissue damage by oxidative stress is a key pathogenic mechanism in various diseases, including AKI and CKD. Thus, early detection of tissue important. Using tRNA-specific modified nucleoside 1-methyladenosine (m1A) antibody, we show that induces direct conformational change tRNA structure promotes subsequent fragmentation occurs much earlier than DNA damage. In models (ischemic reperfusion, toxic injury, irradiation), the levels circulating derivatives increased rapidly. humans, also under...
Hyperuricemia is closely associated with insulin resistance syndrome (and its many cardiometabolic sequelae); however, whether they are causally related has long been debated. We undertook this study to investigate the potential causal nature and direction between hyperuricemia, along gout, by using bidirectional Mendelian randomization (MR) analyses.We used genome-wide association data (n = 288,649 for serum urate [SU] concentration; n 763,813 gout risk; 153,525 fasting insulin) select...