A5060229031- Cancer, Hypoxia, and Metabolism
- Angiogenesis and VEGF in Cancer
- HER2/EGFR in Cancer Research
- RNA Interference and Gene Delivery
- Ovarian cancer diagnosis and treatment
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Advanced Drug Delivery Systems
- Influenza Virus Research Studies
- TGF-β signaling in diseases
- Reproductive Biology and Fertility
- Preterm Birth and Chorioamnionitis
- Glycosylation and Glycoproteins Research
- RNA Research and Splicing
- Lipid metabolism and disorders
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- MicroRNA in disease regulation
- Inflammasome and immune disorders
- Autoimmune and Inflammatory Disorders Research
- Neonatal and fetal brain pathology
- Streptococcal Infections and Treatments
- Circular RNAs in diseases
- Robotics and Automated Systems
- Teleoperation and Haptic Systems
Fukuoka University
2016-2025
Tokyo University of the Arts
2025
The University of Tokyo
2025
Hosei University
2022
Tokyo University of Technology
2022
Scripps Research Institute
2016-2019
Star General Hospital
2018
Setsunan University
2015-2018
National Center For Child Health and Development
2014-2016
National Institute of Technology, Kagoshima College
2016
Aging is a main risk factor for osteoarthritis (OA). FoxO transcription factors protect against cellular and organismal aging, expression in cartilage reduced with aging OA. To investigate the role of cartilage, Col2Cre-FoxO1, 3, 4 single knockout (KO) triple KO mice (Col2Cre-TKO) were analyzed. Articular Col2Cre-TKO Col2Cre-FoxO1 was thicker than control at 1 or 2 months age. This associated increased proliferation chondrocytes vivo vitro. OA-like changes developed synovium, subchondral...
The WW domain-containing protein 2 (Wwp2) gene, the host gene of miR-140, codes for Wwp2 protein, which is an HECT-type E3 ubiquitin ligases abundantly expressed in articular cartilage. However, its function remains unclear. Here, we show that mice lacking and enzyme inactivated (Wwp2-C838A) exhibit aggravated spontaneous surgically induced osteoarthritis (OA). Consistent with this phenotype, WWP2 expression level downregulated human OA We also identify Runx2 as a substrate Adamts5 target...
Abstract Intervertebral disk (IVD) degeneration is a prevalent age‐associated musculoskeletal disorder and major cause of chronic low back pain. Aging the main risk factor for disease, but molecular mechanisms regulating IVD homeostasis during aging are unknown. The aim this study was to investigate function FOXO, family transcription factors linked longevity, in age‐related degeneration. Conditional deletion all FOXO isoforms (FOXO1, 3, 4) using Col2a1Cre AcanCreER mouse resulted...
Recently developed transcription activator-like effector nuclease (TALEN) technology has enabled the creation of knockout mice, even for genes on Y chromosome. In this study, we generated a mouse Sry, sex-determining gene chromosome, using microinjection TALEN RNA into pronuclear stage oocytes. As expected, had female external and internal genitalia, level blood testosterone sexually dimorphic nucleus in brain. The exhibited an estrous cycle performed copulatory behavior as females, although...
Cell differentiation status is defined by the gene expression profile, which coordinately controlled epigenetic mechanisms. type-specific DNA methylation patterns are established chromatin modifiers including de novo methyltransferases, such as Dnmt3a and Dnmt3b. Since discovery of myogenic master MyoD, has been utilized a model system to study tissue differentiation. Although knowledge about networks accumulating, there only limited understanding how controls program. With an aim elucidate...
Although DNA methylation is considered to play an important role during myogenic differentiation, chronological alterations in and gene expression patterns this process have been poorly understood. Using the Infinium HumanMethylation450 BeadChip array, we obtained a profile of genome-wide status human myoblast differentiation model, where myoblasts were cultured low-serum medium stimulate differentiation. As proceeded, their global level increased became more distinct from those mesenchymal...
Heparin-binding EGF-like growth factor (HB-EGF) is one of several proangiogenic factors and represents a possible therapeutic target for patients with triple-negative breast cancer (TNBC). However, the role HB-EGF in promoting tumor aggressiveness TNBC remains unclear. To investigate specific genes pathways involved tumorigenesis, we profiled gene expression changes two cell lines under two-dimensional culture (2DC) three-dimensional (3DC) xenograft model. We identified simultaneous...
Cardiotocography (CTG) has long been the standard method for monitoring fetal status during delivery. Despite its widespread use, human error and variability in CTG interpretation contribute to adverse neonatal outcomes, with over 70% of stillbirths, deaths, brain injuries potentially avoidable through accurate analysis. Recent advancements artificial intelligence (AI) offer opportunities address these challenges by complementing judgment. This study experimentally compared diagnostic...
Abstract Aim This study aimed to assess amniotic fluid bacterial load using the copy number of 16S ribosomal deoxyribonucleic acid and clarify its association with perinatal outcomes. Methods retrospective diagnostic prognostic included 63 patients preterm labor suspected intra‐amniotic infection who underwent amniocentesis before 34 weeks gestation. We measured in 1 mL droplet digital polymerase chain reaction graded infections as Grade (G) 0, 1, 2, or 3. Case–control analysis was performed...
Ovarian cancer is the most lethal gynecologic malignancy. Recently, several molecularly targeted anticancer agents have been developed for ovarian cancer; however, its prognosis remains extremely poor. The development of therapy, as well companion diagnostics, required to improve outcomes patients with cancer. In this study, identify micro RNA s (mi s) involved in progression we analyzed serum mi using array and quantitative RT ‐ PCR examined properties expression cells. cancer, high amount...
Abstract Colorectal cancer ( CRC ) is one of the most frequently occurring cancers with high morbidity and mortality worldwide. Amphiregulin AREG ), a member epidermal growth factor family rational target for therapy, essential three‐dimensional structure tumor formation. To clone genes associated increased expression, we performed cDNA microarray analysis in two cell lines undergoing two‐dimensional (2 DC culture (3 ). Upregulated (>2.0‐fold) downregulated (<0.5‐fold) 3 compared 2...
Interaction of erythropoietin (EPO) and its membrane receptor induces the proliferation differentiation erythroid progenitors. The molecular mechanism EPO receptor-mediated signal transduction remains unclear because cloned does not contain any enzyme-related or nucleotide-binding consensus sequences in cytoplasmic domain. We have previously shown that epidermal growth factor (EGF) dimerization a chimeric carrying extracellular domain EGF linked to receptor, resulting producing signals...
Abstract Intra-amniotic infection (IAI) is a major cause of preterm birth with poor perinatal prognosis. We aimed to determine whether analyzing vaginal microbiota can evaluate the risk chorioamnionitis (CAM) in labor cases. Vaginal discharge samples were collected from 83 pregnant women admitted for labor. Based on Blanc’s classification, participants divided into CAM (stage ≥ II; n = 46) and non-CAM ≤ I; 37) groups. The 16S rDNA amplicons (V1–V2) sequenced analyzed. Using random forest...
Age-related decline in the number of ovulations and ovum quality are major causes female infertility, stem cells have been reported to be effective tissue regeneration. However, current therapeutic modalities inadequate. This study investigated effects adipose-derived mesenchymal (ASCs) on ovarian functions aged mice.
Peptide and protein drugs, which are categorized as biologics, exhibit poor membrane permeability. This pharmacokinetic disadvantage has largely restricted the development of noninvasive dosage forms biologics that deliver into systemic circulation. We have been investigating potential use cell-penetrating peptide-linked polymers a novel absorption enhancer to overcome this challenge. Since our previous study revealed biocompatible poly(N-vinylacetamide-co-acrylic acid) modified with...