A5012744077- Chronic Obstructive Pulmonary Disease (COPD) Research
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Frailty in Older Adults
- Protein Kinase Regulation and GTPase Signaling
- Nutrition and Health in Aging
- Colorectal Cancer Treatments and Studies
- TGF-β signaling in diseases
- COVID-19 Clinical Research Studies
- Intensive Care Unit Cognitive Disorders
- SARS-CoV-2 and COVID-19 Research
- Phonocardiography and Auscultation Techniques
- Respiratory Support and Mechanisms
- Asthma and respiratory diseases
- Lipid metabolism and disorders
- 3D Printing in Biomedical Research
- Ion channel regulation and function
- Cancer Cells and Metastasis
- Respiratory and Cough-Related Research
- Phosphodiesterase function and regulation
- DNA and Nucleic Acid Chemistry
- Long-Term Effects of COVID-19
- RNA modifications and cancer
Yamaguchi University
2020-2024
National Sanyo Hospital
2024
Fukuoka University
2011-2023
Yamaguchi University Hospital
2023
Kyoto University
2009-2015
Kyushu University
2013
Frontier Medical College
2013
Kindai University
2005
Japan Science and Technology Agency
2005
Kobe Pharmaceutical University
2003
Abstract Excess production of reactive oxygen species (ROS) is known to cause apoptotic cell death. However, the molecular mechanisms whereby ROS induce apoptosis remain elusive. Here we show that NHL-repeat-containing protein 2 (NHLRC2) thioredoxin-like domain cleaved by caspase-8 in ROS-induced HCT116 human colon cancer line. Treatment cells with oxidant tert -butyl hydroperoxide (tBHP) induced and reduced NHLRC2 levels, whereas pretreatment antioxidant N -acetyl- l -cysteine prevented...
Heparin-binding EGF-like growth factor (HB-EGF) is one of several proangiogenic factors and represents a possible therapeutic target for patients with triple-negative breast cancer (TNBC). However, the role HB-EGF in promoting tumor aggressiveness TNBC remains unclear. To investigate specific genes pathways involved tumorigenesis, we profiled gene expression changes two cell lines under two-dimensional culture (2DC) three-dimensional (3DC) xenograft model. We identified simultaneous...
Growth and differentiation of colonic epithelium are regulated in the three-dimensional (3D) physiological architecture, crypt, deregulation 3D interactions is involved tumorigenesis. Cell-based culture systems provide a suitable approach bridging gap between two-dimensional (2D) animal models. KRAS mutations found at high frequencies human colorectal cancer (CRC); however, KRAS-targeted therapy has not been developed. Here, we have established cell model resembling crypt by use HKe3 cells,...
Abstract Background We previously established a three-dimensional (3-D) colonic crypt model using HKe3 cells which are human colorectal cancer (CRC) HCT116 with disruption in oncogenic KRAS, and revealed the crucial roles of KRAS both inhibition apoptosis cell polarity; however, molecular mechanism KRAS-induced these 3-D specific biological changes remains to be elucidated. Results Among genes that were upregulated by this model, we focused on phosphodiesterase 4B ( PDE4B ) expression levels...
(1) Background: Reduced lung function in early adulthood is associated with future risks to health outcomes that have not been fully explored by gender. We investigated gender-specific relationships between and extrapulmonary variables, assessing their potential as screening markers for respiratory dysfunction young adults. (2) Methods: The participants were 151 medical students. Clinical data, handgrip strength (HS); body composition parameters such skeletal muscle mass index (SMI),...
ZFAT (also known as ZNF406), originally identified a candidate gene for autoimmune thyroid disease, encodes zinc-finger protein, however, its function has not been elucidated. Here, we report that human protein is expressed in peripheral B and T lymphocytes acute lymphoblastic leukaemia cell line, MOLT-4 cells. Intriguing mouse expression CD4(+) increased during blast formation. Furthermore, ZFAT-knockdown induces apoptosis via activation of caspases. These results suggested critical...
TAL1 plays pivotal roles in vascular and hematopoietic developments through the complex with LMO2 GATA1. Hemangioblasts, which have a differentiation potential for both endothelial lineages, arise primitive streak migrate into yolk sac to form blood islands, where hematopoiesis occurs. ZFAT (a zinc-finger gene autoimmune thyroid disease susceptibility region / an immune-related transcriptional regulator containing 18 C 2 H -type domains one AT-hook) was originally identified as AT-hook, is...
Recent studies suggest that SIPA1 encoding a Rap GTPase-activating protein SPA-1 is candidate metastasis efficiency-modifying gene in human breast cancer. In this study, we investigated the expression and function of prostate cancer (CaP). Immunohistochemical tumor specimens from CaP patients revealed positive correlation with disease progression metastasis. The was recapitulated cell lines; LNCaP rarely showed SCID mice expressed an undetectable level SPA-1, whereas highly metastatic PC3...
Abstract Physical inactivity and cognitive impairment in patients with chronic obstructive pulmonary disease (COPD) can lead to frailty poor prognoses. However, little is known regarding the association between human brain. We hypothesized that brain structure could change according COPD focused on cortical thickness. Cortical thickness measured by magnetic resonance imaging scores using Kihon Checklist (KCL) were assessed 40 stable 20 healthy controls. Among 34 regions assessed, multiple...
Background/Aim: We previously reported the crucial roles of oncogenic Kirsten rat sarcoma viral oncogene homologue (KRAS) in inhibiting apoptosis and disrupting cell polarity via regulation phosphodiesterase type 4B2 (PDE4B2) expression human colorectal cancer (CRC) HCT116 cells a three-dimensional culture (3DC). Here, we evaluated effects apremilast, selective PDE4 inhibitor, on luminal 3DC nude mice assay using HKe3 CRC stably expressing wild-type (wt)PDE4B2 (HKe3-wtPDE4B2), mutant...
Sedentary behavior and cognitive impairment have a direct impact on patients’ outcomes. An energy metabolic disorder may be involved in the overlap of these comorbid conditions (motoric risk (MCR)) patients with chronic obstructive pulmonary disease (COPD). We aimed to explore linkage between proapoptotic protein, growth differentiation factor (GDF)-15, MCR. Physical activity (PA), function (Japanese version Montreal Cognitive Assessment: MOCA-J), serum GDF-15 levels were assessed healthy...
Tespa1 has been recently reported to be a critical molecule in T-cell development, however, the precise molecular mechanisms of remain elusive. Here, we demonstrate that shows amino-acid sequence homology KRAS-induced actin-interacting protein (KRAP), an inositol 1,4,5-trisphosphate receptor (IP3R) binding protein, and physically associates with IP3R T B lymphocytes. Two-consecutive phenylalanine residues (Phe185/Phe186) Tespa1, which are conserved between KRAP, indispensable for association...
Oligonucleotides can be covalently linked to peptides composed of any sequence amino acids by solid phase fragment condensation. The incorporated into the conjugates include nuclear localizing signals (NLS), export (NES), membrane fusion domain some viral proteins and designed with amphipathic character. Evaluation biological properties DNA-peptide indicated that (a) could bind target RNA dsDNA increased affinity, (b) were more resistant cellular nuclease degradation, (c) conjugate-RNA...
Obesity and related metabolic disorders have become leading causes of adult morbidity mortality. KRAP (Ki-ras-induced actin-interacting protein) is a cytoskeleton-associated protein ubiquitous among tissues, originally identified as cancer-related molecule, however, its physiological roles remain unknown. Here we demonstrate that KRAP-deficient (KRAP−/−) mice show enhanced rate, decreased adiposity, improved glucose tolerance, hypoinsulinemia hypoleptinemia. KRAP−/− are also protected...
Forkhead box O1 (FoxO1) is a key molecule for the development and functions of peripheral T cells. However, precise mechanisms regulating FoxO1 expression in cells remain elusive. We previously reported that Zfat(f/f)-CD4Cre mice showed marked decline protein levels cells, partially through proteasomal degradation. Here we have identified mechanisms, apart from proteasome-mediated degradation, decreased Zfat-deficient First, confirmed tamoxifen-inducible deletion Zfat Zfat(f/f)-CreERT2...