Aim . To compare the effects of various mesenchymal stem cells, those isolated from human exfoliated deciduous teeth (SHEDs), dental pulp cells (DPSCs), and follicle (DFSCs), on peripheral blood mononuclear (PBMCs). Method Mesenchymal were three sources in orofacial region. Characterization PCR analyses performed. Lymphocytes healthy venous blood. cocultured with presence absence IFN‐ γ stimulated anti‐CD2, anti‐CD3, anti‐CD28 for 3 days. Then, lymphocyte proliferation, number CD4 + FoxP3 T...

The aim of this study was to investigate the regenerative effect lyophilized dental follicle mesenchymal stem cells (DF-MSCs) combined with rat platelet-rich fibrin (PRF) on geriatric skin wounds. Human DF-MSCs which were isolated from wisdom teeth healthy donors and PRF mixed incubated in a 37 °C incubator for 1–2 h containing 1 million 150 mg PRF. mixture suspended freeze-drying solution then lyophilized. Wounds created back Wistar albino rats using 6 mm punch. Lyophilized DF-MSCs, PRF, or...

Summary Background Asthma is a chronic inflammatory disease in which responses have the polarisation of CD 4 + T cells to Th2 cells. Dental follicle mesenchymal stem ( DFSC s) strong anti‐inflammatory properties comparable other Objective We investigated immunomodulatory effects s on helper cell asthmatic patients and compared results with those obtained subjects immunotherapy healthy individuals. Method Peripheral blood mononuclear PBMC ) were isolated from naïve asthmatics, asthmatics...

Abstract Background This in vitro study examined the effect of inflammatory cytokines (tumour necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6) on osteogenic, chondrogenic, adipogenic differentiation dental pulp stem cells (DPSCs) which have significant relevance future regenerative therapies. Methods DPSCs were isolated from impacted third molar determined with flow cytometry analysis. divided into 5 main groups 3 subdivisions for each group making a total 15 groups. Experimental...

Abstract In this study, the anti‐inflammatory effect of dental pulp mesenchymal stem cell (MSCs) exosomes loaded to mucoadhesive hydrogel is investigated in a nickel hypersensitivity murine model. After culture MSCs third passage are based on chitosan, cross‐linked with genipin and modified catechol groups. A model created by administering NiCl 2 10 µg mL −1 lipopolysaccharide 4–6 week‐old Balb‐c mice intradermal injection. treated MSC hydrogel, interferron gamma (IFN‐γ) secreting CD4+T...

Background: Breast cancer is a common with high mortality rates. Early diagnosis crucial for reducing the prognosis and Therefore, development of alternative treatment options necessary. Objective: This study aimed to investigate inhibitory effect N-acetyl-D-glucosamine (D-GlcNAc) on breast using machine learning method. The findings were further confirmed through assays cell lines. Methods: MCF-7 4T1 lines (ATCC) cultured in presence absence varying concentrations D-GlcNAc (0.5 mM, 1 2 4...

Objective Dental mesenchymal stem cells (MSCs) are potential for use in tissue regeneration inflammatory diseases due to their rapid proliferating, multilineage differentiation, and strong anti-inflammatory features. In the present study, immunoregulatory glandular effects of dental follicle (DF)MSCs Sjögren’s Syndrome (SS) were investigated. Methods (DF) tissues obtained from healthy individuals during tooth extraction, digested enzymatically DFMSCs cultured until third passage. labeled...

Background: In this article, the authors investigate modulatory effects of dental mesenchymal stem cells (MSCs) on lymphocyte responses in primary Sjögren's syndrome (pSS), which is an autoimmune disease resulting from keratoconjunctivitis sicca and xerostomia. Methods: Mononuclear isolated pSS patients cultured with or without MSCs analyzed for via flow cytometry. Results: Dental-follicle (DF)- dental-pulp (DP)-MSCs downregulated CD4+ T proliferation by increasing Fas-ligand expression...

Background: Mesenchymal stem cells (MSCs) have gained remarkable attention because of their ability to dualistically regulate tumor growth. The main objective this study was evaluate the apoptotic effects human bone marrow-derived (hBM) MSCs in combination with interferon gamma (IFN-γ) on MCF-7 breast cancer cells, and determine cytokines involved process. Materials Methods: hBM-MSCs were co-cultured either directly indirectly for 72 h in-vitro. Levels necrosis factor-related...

Background/Aims: Crohn's disease (CD) is a chronic inflammatory condition characterized by various abnormalities that lead to overly aggressive T cell responses.Our in vitro experiments aimed investigate the potential use of Dental Follicle Mesenchymal Stem Cells (DF-MSCs) suppress exaggerated immune response inflamed and noninflamed tissue CD. Materials Methods: follicle tissues were obtained from extracted third molar teeth three healthy volunteers who had no abscess or diseases.In total,...

Asthma is a chronic inflammatory disease of the airways where exaggerated T helper 2 immune responses and mediators play role. Current asthma treatment options can effectively suppress symptoms control process; however, cannot modulate dysregulated response. Allergen-specific immunotherapy one effective treatments capable modification. Injecting allergens under skin in allergen-specific reduce improve sensitivity lungs, has risk severe reactions. Mesenchymal stem cells have immunoregulatory...

Allergen-specific immunotherapy to induce T regulatory cells in the periphery has been used treat allergic diseases. Mycobacteria can be as an adjuvant for inducing cells. However, it is unclear whether intranasal combination with induces cell differentiation and attenuates responses vivo. To investigate role of ovalbumin (OVA) treatment alone vaccae, proportions FoxP3+ anti-inflammatory were evaluated a murine model asthma that was established three groups bicistronic Foxp3EGFP reporter...

Ectoine is an amino acid that can preserve osmotic balance and has more preservative activity than other osmoregulators. There are no published reports on the osmoregulators' effects viability or differentiation of dental stem cells. The aim this study was to investigate effect as a storage media potential human periodontal ligament mesenchymal cells (hPDLMSCs).hPDLMSCs were obtained from impacted third molar teeth. isolated, submitted trilineage differentiation, characterized by flow...

Background: Atopic dermatitis (AD) is an inflammatory cutaneous disorder. The advancements in the understanding of AD immunological pathogenesis have caused development therapies that suppress dysregulated immune response. We aimed to evaluate immunomodulatory effect dental stem cells (dental follicle-mesenchymal [DF-MSCs]) on patients. Materials & methods: investigated immunoregulatory potential DF-MSCs T cell response and compared them with psoriasis healthy individuals underlying...

Objectives: In this study, we aimed to investigate the differentiation potential of dental follicle mesenchymal stem cells (MSCs) in synovial fluid (SF) niche early-onset or end-stage rheumatoid arthritis (RA). Patients and methods: Between May 2020 January 2021, six patients (1 male, 5 females; mean age: 57.5±11.2 years; range, 49 65 years) who were diagnosed with RA indication SF aspiration included study. The third passage (DFSCs) cocultured fresh samples micromass culture system for 21...

<title>Abstract</title> The aim of this study was to investigate the regenerative effect lyophilized dental follicle mesenchymal stem cells (DF-MSCs) combined with platelet-rich fibrin (PRF) on geriatric skin wounds. Human DF-MSCs isolated from healthy donors and PRF were mixed incubated in a 37°C incubator for 1–2 hours containing 1 million 150 mg PRF. mixture suspended freeze-drying solution then lyophilized. Wounds created back Wistar albino rats using 6 mm punch. Lyophilized DF-MSCs,...

Background: Neuropathic pain remains a persistent clinical problem and characterized by mechanical allodynia heat hyperalgesia. Chronic conditions are among the major health problems which difficult to treat. Bone marrow-derived mesenchymal stromal cells (BMSCs) have generated great interest as an option for cell-based therapy. BMSCs easy isolate expand ex vivo. Clinical studies show that direct injection of does not produce side effects rejection is well tolerated immune system. Methods:...

Objective: The purpose of our study is to investigate the immunomodulatory effects Dental Follicle Mesenchymal Stem Cells (DF-MSCs) on lymphocytes isolated from peripheral blood AD patients, a Th2 disease and psoriasis, Th1 / Th17 compare them with healthy individuals in vitro.Methods: Patients (n = 9) psoriasis 6) who are followed up Marmara University Pediatric Allergy Immunology Dermatology outpatient clinics subjects were included. Peripheral Blood Mononuclear (PBMCs) 20 ml venous all...

Background/aim: Multipotent mesenchymal stem cells (MSCs) have been investigated in autoimmune diseases such as rheumatoid arthritis (RA) due to their immunomodulatory and regenerative properties. In this study, immunosuppressive effects on peripheral blood mononuclear (PBMC) of RA patients were studied.Materials methods: Dental follicle (DFSCs) isolated from tissue the orofacial region. Characterization multipotency analyses performed. Lymphocytes venous (n = 5) healthy individuals 5)....

<title>Abstract</title> Background Dental pulp stem cells (DPSCs) are of special relevance in future regenerative dental and immunological medical therapies due to their differentiation immunologic potentials. This<italic>in vitro</italic>study examined the effect inflammatory cytokines tumour necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6 on osteogenic, chondrogenic, adipogenic DPSCs. Methods DPSCs were isolated from impacted third molar determined with flow cytometry analysis....