A5061517006- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Ovarian cancer diagnosis and treatment
- Cutaneous Melanoma Detection and Management
- Cancer Immunotherapy and Biomarkers
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- Cancer Genomics and Diagnostics
- Virus-based gene therapy research
- Cancer Research and Treatments
- Genetic factors in colorectal cancer
- Neuroblastoma Research and Treatments
- Reproductive System and Pregnancy
- Sarcoma Diagnosis and Treatment
- Bone Tumor Diagnosis and Treatments
- Lung Cancer Treatments and Mutations
- Acute Myeloid Leukemia Research
- Immune Cell Function and Interaction
- Hematological disorders and diagnostics
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Renal cell carcinoma treatment
- Endometrial and Cervical Cancer Treatments
- Polyomavirus and related diseases
The University of Texas MD Anderson Cancer Center
2019-2025
Rutgers, The State University of New Jersey
2023-2024
Scripps MD Anderson Cancer Center
2023
University of Wisconsin–Madison
2008-2020
Madison Group (United States)
2017
Heidelberg University
2013
German Cancer Research Center
2013
Centro Regional de Derechos Humanos y Justicia de Género, Corporación Humanas
2012
University of Wisconsin Carbone Cancer Center
2012
Jilin Province Tumor Hospital
2011
Summary The abundance of six tetracycline resistance genes tet (O) , (Q) (W) (M), (B) and (L), were quantified over time in wastewater lagoons at concentrated animal feeding operations (CAFO) to assess how feedlot operation affects downstream surface waters. Eight five cattle feedlots the Midwestern United States monitored for 6 months. Resistance 16S‐rRNA gene abundances using real‐time PCR, physicochemical lagoon conditions, levels, other factors (e.g. size weather conditions) time....
Hypotonic stimulation induces airway constriction in normal and asthmatic airways. However, the osmolarity sensor has not been characterized. TRPV4 (also known as VR-OAC, VRL-2, TRP12, OTRPC4), an osmotic-sensitive cation channel transient receptor potential (TRP) family, was recently cloned. In present study, we show that mRNA expressed cultured human smooth muscle cells analyzed by RT-PCR. induced Ca 2+ influx osmolarity-dependent manner, consistent with reported biological activity of...
A new real-time PCR method is presented that detects and quantifies three tetracycline resistance (Tcr) genes [tet(O), tet(W), tet(Q)] in mixed microbial communities resident feedlot lagoon wastewater. Tcr gene TaqMan primer-probe sets were developed optimized to quantify the present seven different cattle lagoons, validate method, assess whether concentrations correlate with free-tetracycline levels waters. The proved be sensitive across a wide range of provided consistent reproducible...
Response to immunocytokine (IC) therapy is dependent on natural killer cells in murine neuroblastoma (NBL) models. Furthermore, immunoglobulin-like receptor (KIR)/KIR-ligand mismatch associated with improved outcome autologous stem cell transplant for NBL. Additionally, clinical antitumor response monoclonal antibodies has been specific polymorphic-FcγR alleles. Relapsed/refractory NBL patients received the hu14.18-IL2 IC (humanized anti-GD2 antibody linked human IL2) a Children's Oncology...
Interest in combining radiotherapy and immune checkpoint therapy is growing rapidly. In this study, we explored a novel combination of type to augment antitumor responses preclinical murine models melanoma, neuroblastoma, head neck squamous cell carcinoma. Cooperative effects were observed with local intratumoral injection tumor-specific antibodies, arising part from enhanced antibody-dependent cell-mediated cytotoxicity (ADCC). We could improve response by radiation an IL2-linked antibody...
Abstract Introduction This study aimed to assess the feasibility of implementing Idylla system, an ultra‐rapid, cartridge‐based assay, as extension rapid on‐site evaluation (ROSE) in cytology. The authors conducted a pilot validation on specimens from non–small cell lung carcinoma, thyroid and melanoma, evaluating four assays designed detect alterations KRAS , EGFR BRAF gene fusions, expression imbalances ALK ROS1 RET NTRK 1/2/3, MET exon 14 skipping transcripts. They investigated providing...
Abstract hu14.18–IL-2 (IC) is an immunocytokine consisting of human IL-2 linked to hu14.18 mAb, which recognizes the GD2 disialoganglioside. Phase 2 clinical trials i.v. (i.v.-IC) in neuroblastoma and melanoma are underway have already demonstrated activity neuroblastoma. We showed previously that intratumoral (IT-IC) results enhanced antitumor mouse models compared with i.v.-IC. The studies presented this article were designed determine mechanisms involved support future testing...
The prognosis for advanced neuroblastoma remains poor with high risk of recurrence after consolidation.Therapies based on monoclonal antibodies that specifically target disialoganglioside GD2 tumor cells are improving treatment results high-risk neuroblastoma.This article reviews the use anti-GD2 either as monotherapy or part a larger and more complex approach neuroblastoma.We review how can be combined other treatments strategies to enhance their clinical effects.Tumor resistance problems...
We aimed to describe RAS mutations in gynecologic cancers as they relate clinicopathologic and genomic features, survival, therapeutic implications.
Abstract: Gene rearrangements or fusions have emerged as critical oncogenic drivers in various cutaneous adnexal neoplasms. This review offers a comprehensive overview of both established and recently identified molecular alterations, with specific focus on gene fusions. Key alterations discussed include YAP1 rearrangements, CRTC1::MAML2 fusions, BRD3 MYB::NFIB ETV6::NTRK3 PLAG1 alongside rarer fusion transcripts, such MEF2C::SS18 , FOXK1::GRHL1/2 GPS2::GRHL RARA::NPEPPS . The article...
Lynch syndrome (LS), caused by inherited mutations in DNA mismatch repair genes including MSH2, carries a 60% lifetime risk of developing endometrial cancer (EC). Beyond hypermutability, mechanisms driving LS-associated EC remain unclear. We investigated MSH2 loss pathogenesis using mouse model (PR-Cre Msh2LoxP/LoxP, abbreviated Msh2KO), primary cell lines, human tissues, and cells with isogenic knockdown. By eight months, 58% Msh2KO mice developed atypical hyperplasia (AH), precancerous...
Merkel cell carcinomas (MCCs) exhibit diverse molecular profiles, often categorized by their association with polyoma virus (MCPyV). MCPyV-associated MCCs typically display a low tumor mutational burden (TMB), lacking both somatic mutations and ultraviolet signature. By contrast, MCPyV-negative commonly arise in sun-exposed skin frequently high TMB, along TERT promoter mutation (TPM) mutations, particularly TP53 RB1. Gene fusions are exceedingly rare MCCs, specific frequency fusion...
B-Raf Proto-Oncogene (BRAF) fusions are rare in melanomas. We present a case of cutaneous melanoma with Ring Finger Protein 11 (RNF11)::BRAF fusion 63-year-old man history stage IB on the right upper back (pT2apN0cM0; nonulcerated Breslow thickness 1.2 mm). Despite initial treatment, progressed to multiple metastases. Histopathologically, tumor cells exhibited epithelioid and rhabdoid morphologies, occasional giant pleomorphic multinucleation, were positive for antimelanocytic cocktail...
PURPOSE A 70-gene liquid biopsy (LB) panel (LBP-70) was implemented at our institution to identify genetic alterations in the plasma of patients with solid tumors. We report clinical utility LBP-70 a retrospective study 1,243 consecutively tested patients. MATERIALS AND METHODS Electronic medical records were reviewed for relevant clinicopathologic and radiologic information preanalytical variables, including circulating cell-free DNA yield. The assay evaluated on basis its ability major...
ALK-fused Spitz melanocytic neoplasms are a distinct subgroup of lesions exhibiting unique histopathologic characteristics. These often manifest as exophytic or polypoid tumors, characterized by fusiform-to-epithelioid melanocytes arranged in nested, fascicular, plexiform growth pattern. Several fusion partners the ALK gene have been identified spitzoid neoplasms, with TPM3 and DCTN1 being most prevalent. Less common include NPM1, TPR, CLIP1, GTF3C2, EEF2, MYO5A, KANK1, EHBP1. The MLPH gene,...
Gene fusions have emerged as crucial molecular drivers of oncogenesis in a subset cutaneous adnexal neoplasms, including poroid neoplasms and hidradenomas. We present unique case primary apocrine carcinoma harboring RARA::NPEPPS fusion, broadening the spectrum fusion-associated neoplasms. A 77-year-old African American male presented with an ulcerated thigh nodule. Histopathologically, predominantly dermal-based adenocarcinoma exhibited papillary, micropapillary, cribriform, solid growth...
Colorectal cancer (CRC) is one of the most common worldwide. However, a large number genetic risk factors involved in CRC have not been understood. Copy variations (CNVs) might partly contribute to 'missing heritability' CRC. An increased overall burden CNV has identified several complex diseases, whereas association between and largely unknown. We performed genome-wide investigation CNVs on genomic DNA from 384 familial cases 1285 healthy controls by Affymetrix 6.0 array. increase was...