A5072687747- Acute Myeloid Leukemia Research
- CAR-T cell therapy research
- Chronic Myeloid Leukemia Treatments
- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Immune Cell Function and Interaction
- Multiple Myeloma Research and Treatments
- Viral-associated cancers and disorders
- Genetic factors in colorectal cancer
- Ubiquitin and proteasome pathways
- Cutaneous lymphoproliferative disorders research
- Advanced biosensing and bioanalysis techniques
- Protein Degradation and Inhibitors
- Cancer Immunotherapy and Biomarkers
- Acute Lymphoblastic Leukemia research
- Eosinophilic Disorders and Syndromes
- Immunodeficiency and Autoimmune Disorders
- Histiocytic Disorders and Treatments
- Cancer-related Molecular Pathways
- Lung Cancer Treatments and Mutations
- MicroRNA in disease regulation
The University of Texas MD Anderson Cancer Center
2019-2025
Scripps MD Anderson Cancer Center
2024
Hofstra University
2015-2017
Northwell Health
2014
Abstract Interferon gamma (IFNγ) is a critical cytokine known for its diverse roles in immune regulation, inflammation, and tumor surveillance. However, while IFNγ levels were elevated sera of most newly diagnosed acute myeloid leukemia (AML) patients, complex interplay AML remains insufficiently understood. We aim to characterize these interactions through comprehensive bulk single-cell approaches bone marrow patients. identify monocytic as having unique microenvironment characterized by...
In the context of kidney transplantation, genomic incompatibilities between donor and recipient may lead to allosensitization against new antigens. We hypothesized that recessive inheritance gene-disrupting variants represent a risk factor for allograft rejection.We performed two-stage genetic association study rejection. first stage, we screen 50 common gene-intersecting deletion polymorphisms in cohort transplant recipients. second replicated our findings three independent cohorts...
Previously considered rare, inherited hematologic malignancies are increasingly identified. Germline mutations in the RNA helicase DDX41 predispose to increased lifetime risks of myeloid neoplasms with disease often occurring later life which presents challenges for germline recognition. To improve identification DDX41, individuals presenting ≥1 alteration on an institutional MDS/AML next-generation sequencing based panel at least one >40% variant allele frequency were flagged review and...
Prompt diagnosis of acute promyelocytic leukemia (APL) is critical for patient care. In this study, we aimed to characterize the immunophenotype APL and explore immunophenotypic difference between its mimics using flow cytometric analysis.Eighty-five cases were collected, including 47 APL, 26 NPM1-mutated myeloid (AML) 12 KMT2A-rearranged AML with an APL-like immunophenotype. Immunophenotypes analyzed analysis.APL showed four distinct patterns (designated a-d) based on CD45/SSC plots. Blasts...
Comprehensive investigation of CD8+ T cells in acute myeloid leukemia (AML) is essential for developing immunotherapeutic strategies beyond immune checkpoint blockade. Herein, we performed single-cell RNA profiling from 3 healthy bone marrow donors and 23 newly diagnosed (NewlyDx) 8 relapsed/refractory (RelRef) AML patients. Cells co-expressing canonical exhaustion markers formed a cluster constituting <1% all cells. We identified two effector cell subsets characterized by distinct cytokine...
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In patients with multiple myeloma, plasmablastic transformation in the bone marrow is rare and associated poor outcomes. The significance of discordant extramedullary small, mature clonal plasma cells has not been well studied. Here, we report clinicopathologic, cytogenetic, molecular features 10 such (male/female: 6/4, median age: 65 y, range: 48 to 76 y) an established diagnosis myeloma composed parallel a concurrent or subsequent transformation. Eight available survival data showed...
PURPOSE A 70-gene liquid biopsy (LB) panel (LBP-70) was implemented at our institution to identify genetic alterations in the plasma of patients with solid tumors. We report clinical utility LBP-70 a retrospective study 1,243 consecutively tested patients. MATERIALS AND METHODS Electronic medical records were reviewed for relevant clinicopathologic and radiologic information preanalytical variables, including circulating cell-free DNA yield. The assay evaluated on basis its ability major...
Acute myeloid leukemia (AML) with RUNX1::RUNX1T1 fusion is well known to often demonstrate aberrant upregulation of CD19 expression. We studied the clinicopathologic and genetic features 16 cases AML various RUNX1 lesions, including mutations, copy number gains, translocations other than fusions RUNX1T1. Most these were classified as AML-myelodysplasia-related or AML-post-cytotoxic therapy based on cytogenetic molecular work-up. These neoplasms showed partial expression one more B-cell...
ABSTRACT Acute undifferentiated leukemia (AUL) is a rare hematologic malignancy lacking lineage‐specific markers. Concurrent, clonally related AUL and T‐lymphoblastic lymphoma (T‐LBL) has not been reported previously. Here we describe patient who was diagnosed with in the bone marrow T‐LBL mediastinum after thorough immunophenotyping by flow cytometry immunohistochemistry. Despite their immunophenotypic differences, showed identical genetic alterations: SET::NUP214 fusion, PHF6 , EZH2...
Abstract BACKGROUND Mutations in the RAS‐MAPK pathway, such as KRAS , NRAS and BRAF are known high‐risk factors associated with poor prognosis patients various cancers, but studies myeloma have yielded mixed results. METHODS We describe clinicopathologic, cytogenetic, molecular features, outcomes of 68 RAS/BRAF ‐mutated myeloma, compare 79 without any mutations. RESULTS show that were mutated 16%, 11%, 5% cases, respectively. had lower hemoglobin platelet counts, higher levels serum lactate...
Acute myeloid leukemia (AML) is a heterogeneous malignancy of the blood primarily treated with intensive chemotherapy. The allogeneic T-cell antileukemic activity via donor lymphocyte infusions and stem cell transplantation suggests potential role for checkpoint blockade therapy in AML. While clinical trials employing these treatments have fallen short expected results, deeper exploration into functional states T cells AML could bridge this knowledge gap. In study, we analyzed polyfunctional...
Abstract Acute myeloid leukemia (AML) is a heterogeneous disease characterized by high rate of therapy resistance. Since the cell origin can impact response to therapy, it crucial understand lineage composition AML cells at time Here we leverage single-cell chromatin accessibility profiling 22 bone marrow aspirates from eight patients resistance and following subsequent characterize their landscape. Our findings reveal complex architecture therapy-resistant that are primed for stem...
// Richard K. Yang 1 , Yun Qing 2 Fatima Zahra Jelloul Mark J. Routbort Peng Wang Kenna Shaw 3 Jiexin Zhang 4 Jack Lee L. Jeffrey Medeiros Scott Kopetz 5 Michael T. Tetzlaff 6 and Russell R. Broaddus 7 Department of Hematopathology, University Texas MD Anderson Cancer Center, Houston, TX, USA Biostatistics, Institute for Personalized Treatment (IPCT), Bioinformatics Computational Biology, Gastrointestinal Medical Oncology, Departments Anatomical Pathology Translational Molecular Pathology,...
This report highlights the value of flow cytometry analysis, particularly in setting myeloproliferative neoplasms showing features progression, as neoplastic plasmacytoid dendritic cell (PDC) proliferations may be present, representing either a clonal expansion mature PDCs related to underlying neoplasm or transformation blastic (BPDCN). BPDCN should always considered patients with myeloid progression and/or who develop new cutaneous findings, it prompt change management.
<div>Abstract<p>Comprehensive investigation of CD8+ T cells in acute myeloid leukemia (AML) is essential for developing immunotherapeutic strategies beyond immune checkpoint blockade. Herein, we performed single-cell RNA profiling from 3 healthy bone marrow donors and 23 newly diagnosed (NewlyDx) 8 relapsed/refractory (RelRef) AML patients. Cells co-expressing canonical exhaustion markers formed a cluster constituting <1% all cells. We identified two effector cell subsets...
ETNK1 mutation has been suggested as a useful tool to support the diagnosis of atypical chronic myeloid leukemia. mutations, however, occur in other neoplasms.The authors assessed clinicopathologic and molecular genetic features 80 ETNK1-mutated neoplasms.Thirty-seven neoplasms (46%) were classified myelodysplastic syndrome, 17 (21%) myelodysplastic/myeloproliferative neoplasm, 14 (18%) acute leukemia, 12 (15%) myeloproliferative neoplasm. mutations detected at first test 96% patients,...
Esophageal schwannoma is a rare tumor first described in 1967 by Chaterlin and Fissore. These tumors are most commonly found incidentally or from diagnostic work up of dysphagia dyspnea. This entity cannot be diagnosed on clinical radiographic basis alone. Histology demonstrates palisading spindle cells, few if any mitotic figures, peripheral cuff lymphoid cells. Immunohistochemically, cells stain positive for S100, characteristic marker Schwann Once diagnosed, surgical enucleation the...