A5033783347- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Neurotransmitter Receptor Influence on Behavior
- Memory and Neural Mechanisms
- Photoreceptor and optogenetics research
- Neuroscience of respiration and sleep
- Stress Responses and Cortisol
- Neural dynamics and brain function
- Ion channel regulation and function
- Neuropeptides and Animal Physiology
- Advanced MRI Techniques and Applications
- Neuroendocrine regulation and behavior
- Mitochondrial Function and Pathology
- Functional Brain Connectivity Studies
- Sulfur Compounds in Biology
- Receptor Mechanisms and Signaling
- Water Quality Monitoring and Analysis
- Infant Health and Development
- Asthma and respiratory diseases
- Inhalation and Respiratory Drug Delivery
- Advanced Neuroimaging Techniques and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurological disorders and treatments
- Molecular Sensors and Ion Detection
University of North Carolina at Chapel Hill
2017-2024
Charité - Universitätsmedizin Berlin
2013-2024
Humboldt-Universität zu Berlin
2019-2024
Freie Universität Berlin
2024
Scripps Research Institute
2015
Bioscience Research
2014
Institut Interdisciplinaire de Neuroscience
2010-2011
Oregon Health & Science University
2007-2011
Georgetown University
2008-2010
Georgetown University Medical Center
2008
Synaptic glutamate transients resulting from vesicular exocytosis are superimposed on a low baseline concentration of in the extracellular space. Reported values concentrations range up to 4 μ m . If were present tonically at micromolar concentrations, many receptors, especially high-affinity NMDA receptors (NMDARs), would be activated or desensitized, altering neuronal excitability. Using NMDARs expressed by CA1 pyramidal cells acute hippocampal slices monitor glutamate, we find that its is...
E-cigarettes vaporize propylene glycol/vegetable glycerin (PG/VG), nicotine, and flavorings. However, the long-term health effects of exposing lungs to vaped e-liquids are unknown.
The vesicular glutamate transporter (VGLUT) plays an essential role in synaptic transmission by filling vesicles with glutamate. At mammalian synapses, VGLUT expression level determines the amount of packaged into vesicles, and specific paralog expressed affects release probability. In this study, we investigate whether there is a link between number VGLUTs on We used combination electrophysiology imaging techniques cultured mouse hippocampal neurons where has been severely altered. found...
Synaptic vesicles undergo sequential steps in preparation for neurotransmitter release. Individual SNARE proteins and the complex itself have been implicated these processes. However, discrete effects of on synaptic function difficult to assess using complete loss-of-function approaches. We therefore used a genetic titration technique cultured mouse hippocampal neurons evaluate contribution neuronal protein Syntaxin1 (Stx1) vesicle docking, priming, release probability. generated graded...
Efficient neurotransmitter release at the presynaptic terminal requires docking of synaptic vesicles to active zone membrane and formation fusion-competent near voltage-gated Ca 2+ channels. Rab3-interacting molecule (RIM) is a critical organizer, as it recruits channels activates vesicle priming via Munc13-1. However, our knowledge about Munc13-independent contributions RIM functions limited. To identify that are solely mediated by RIM, we used genetic manipulations control Munc13-1...
It has been proposed that there is an "apparent monosynaptic" connection between gastric vagal afferent nerve terminals and inhibitory projection neurons in the nucleus tractus solitarius (NTS) two efferent parallel pathways from dorsal motor of vagus (DMV) influence peripheral organs associated with these reflexes (6). The purpose our study was to verify validity views as they relate basal control motility. To test a direct (known release l-glutamate) directly impacting second-order...
EDITORIAL article Front. Integr. Neurosci., 19 March 2015 https://doi.org/10.3389/fnint.2015.00018
The identity of the vesicle neurotransmitter transporter expressed by a neuron largely corresponds with primary that cell releases. However, vesicular glutamate subtype 3 (VGLUT3) is mainly in non-glutamatergic neurons, including cholinergic, serotonergic, or GABAergic neurons. Though functional role for release from these neurons has been demonstrated, interplay between VGLUT3 and neuron's characteristic transporter, particularly case at synaptic level less clear. In this study, we explore...
Background The prefrontal cortex ( PFC ) acts as an integrative hub for the processing of cortical and subcortical input into meaningful efferent signaling, permitting complex associative behaviors. dysfunction is consistently observed with ethanol (EtOH) dependence a core component pathology alcohol use disorders in current models addiction. While intracortical gamma‐aminobutryric acid (GABA)ergic neurotransmission understood to be essential maintaining coordinated network activity within...
Resting-state functional magnetic resonance imaging (fMRI) has drastically expanded the scope of brain research by advancing our knowledge about topologies, dynamics, and interspecies translatability networks. Several databases have been developed shared in accordance with recent key initiatives rodent fMRI community to enhance transparency, reproducibility, interpretability data acquired at various sites. Despite these pioneering efforts, one notable challenge preventing efficient...
Striatal output pathways are known to play a crucial role in the control of movement. One possible component for shaping synaptic striatal neuron is glutamatergic input that originates from cortex and thalamus. Although reports focusing on quantifying glutamatergic-induced morphological changes striatum exist, regulating function remains poorly understood. Using primary neurons newborn mice either sex reduced two-neuron microcircuit culture system, we examined whether modulates neurons. We...
Neural circuits are composed of mainly glutamatergic and GABAergic neurons, which communicate through synaptic connections. Many factors instruct the formation function these synapses; however, it is difficult to dissect contribution intrinsic cell programs from that extrinsic environmental effects in an intact network. Here, we perform paired recordings two-neuron microculture preparations mouse hippocampal neurons investigate how input output two principal cells develop. In our reduced...
Reported values of extracellular glutamate concentrations in the resting state depend on method measurement and vary ∼1000-fold. As levels micromolar range can cause receptor desensitization excitotoxicity, thus affect neuronal excitability, an accurate determination ambient is important. Part variability previous measurements may have resulted from sampling different compartments, e.g., synaptic versus extrasynaptic volumes. A steep concentration gradient between these two compartments...
We examined the effects of altering mu-opioid receptor (MOR) activity in medial subnucleus tractus solitarius (mNTS) on several gastric end points including intragastric pressure (IGP), fundus tone, and receptive relaxation reflex (RRR). Microinjection MOR agonist [d-Ala(2),MePhe(4),Gly(ol)(5)]enkephalin (DAMGO; 1-10 fmol) into mNTS produced dose-dependent decreases IGP. endogenous agonists endomorphin-1 endomorphin-2 (20 mimicked 10 fmol DAMGO. 1 100 pmol DAMGO a triphasic response...
At presynaptic terminals, neurotransmitters are released by synaptic vesicle exocytosis at the active zone. In order to maintain efficient neurotransmission and proper structure, sites of fusion must be cleared rapidly endocytosis. Therefore, coupling exo- endocytosis is crucial. Despite many years research, exact molecular biophysical requirements for remain unclear. We investigate whether can triggered in a calcium-independent fashion evoking using hypertonic sucrose solution. demonstrate...
Our laboratory previously reported that gastric activity is controlled by a robust GABA(A) receptor-mediated inhibition in the medial nucleus of tractus solitarius (mNTS) (Herman et al. 2009), and μ-opioid receptor activation inhibits tone suppression this GABA signaling 2010). These data raised two questions: 1) whether any was due to tonic conductance mNTS; 2) suppressed both phasic signaling. In whole cell recordings from rat mNTS neurons, application three antagonists (gabazine,...
Quantitative analysis of innervation to dorsal motor nucleus the vagus (DMV) fundus-projecting neurons indicates that approximately 17% input are noradrenergic. To determine whether this small percentage innervating DMV output stomach is physiologically relevant, we evaluated role norepinephrine at in mediating a vagovagal reflex controlling fundus. A strain gauge was sutured onto fundus isoflurane-anesthetized rats monitor changes tone evoked by esophageal distension (ED). ED produced...
The release of neurotransmitters at central synapses is dependent on a cascade protein interactions, specific to the presynaptic compartment. Amongst those dedicated molecules cytosolic complexins play an incompletely defined role as synaptic transmission regulators. Complexins are multidomain SNARE complex binding proteins which confer both inhibitory and stimulatory functions. Using systematic mutagenesis combining reconstituted in vitro membrane fusion assays with electrophysiology...
The central nucleus of the amygdala (CeA) is implicated in alcohol use disorder (AUD) and AUD-associated plasticity. CeA a primarily GABAergic that subdivided into lateral medial compartments with genetically diverse subpopulations. GABA A receptors are heteromeric pentamers subunits conferring distinct physiological characteristics. receptor signaling has been ethanol-associated plasticity; however, population-specific changes inhibitory subunit expression remain unclear. Here, we combined...
Synaptic transmission requires the presynaptic release of neurotransmitter from synaptic vesicles (SVs) onto postsynaptic neuron. Vesicular transporter proteins, which use a V-ATPase-generated proton gradient, play crucial role in packaging into SVs. Recent work has revealed different dynamics SVs expressing vesicular glutamate (VGLUT) or GABA (VGAT) proteins. At whole synapse level, this results steady-state pH and reacidification during SV recycling (Egashira et al., 2016). In isolated...