A5003678403- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Protein Hydrolysis and Bioactive Peptides
- Probiotics and Fermented Foods
- Andrographolide Research and Applications
- Neuroscience and Neuropharmacology Research
- Phytochemistry and biological activities of Ficus species
- Antibiotic Resistance in Bacteria
- Protein Structure and Dynamics
- Cancer therapeutics and mechanisms
- Hemoglobinopathies and Related Disorders
- Lipid Membrane Structure and Behavior
- Antibiotics Pharmacokinetics and Efficacy
- Obstructive Sleep Apnea Research
- Proteins in Food Systems
- Peroxisome Proliferator-Activated Receptors
- RNA modifications and cancer
- Hepatitis C virus research
- Airway Management and Intubation Techniques
- Chemotherapy-induced cardiotoxicity and mitigation
- Neuroscience of respiration and sleep
- Lipid metabolism and biosynthesis
- Liver Disease Diagnosis and Treatment
- PI3K/AKT/mTOR signaling in cancer
- Animal Diversity and Health Studies
Kamdhenu University
2024-2025
Chhattisgarh Swami Vivekanand Technical University
2020-2024
Emory University
2023
Charotar University of Science and Technology
2023
University of Pittsburgh Medical Center
2022
Piramal (India)
2020-2021
Royal National Throat Nose and Ear Hospital
2006-2020
Keck Hospital of USC
2020
Rice University
2016-2017
Rensselaer Polytechnic Institute
2006
Activation of the visual pigment rhodopsin is caused by 11-cis to -trans isomerization its retinal chromophore. High-resolution solid-state NMR measurements on both and metarhodopsin II intermediate show how disrupts helix interactions that lock receptor off in dark. We made 2D dipolar-assisted rotational resonance between 13 C-labels chromophore specific tyrosine, glycine, serine, threonine binding site rhodopsin. The essential aspects trajectory are a large rotation C20 methyl group toward...
This study investigated the synthesis of bioactive peptides from sheep milk through fermentation with Limosilactobacillus fermentum KGL4 MTCC 25515 strain and assessed lipase inhibition, ACE α-glucosidase α-amylase inhibition activities during process. The observed highest activities, reaching 74.82%, 70.02%, 72.19%, 67.08% (lipase inhibition) after 48 h at 37°C, respectively. Growth optimization experiments revealed that a 2.5% inoculation rate time resulted in proteolytic activity 9.88...
Abstract Background This study aims to evaluate the antidiabetic and anti‐inflammatory activities of Lacticaseibacillus rhamnosus (M9) MTCC 25516 during fermentation whey soy protein isolates. It also seeks characterize profiles, identify multifunctional peptides, assess structural changes using sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE), two‐dimensional (2D) electrophoresis, Fourier‐transform infrared (FTIR) spectroscopy, confocal laser scanning microscopy (CLSM)....
Introduction: This study aims to valorise cheese whey waste by converting it into bioactive peptides that have several health benefits, potentially leading the development of nutraceuticals and functional foods also used in pharmaceutical industry. Methods: The evaluates antidiabetic, antioxidative, anti-inflammatory properties fermented with Limosilactobacillus fermentum (M4), along production antioxidative antidiabetic peptides. SDS PAGE 2D were performed identify proteins molecular weight...
Abstract The aim of the study is to explore biofunctional properties (antioxidative, anti‐diabetic, and anti‐inflammatory) with release bioactive peptides from fermented camel milk Gir cow through yeast‐lactic fermentation. Fermented exhibited higher antioxidative, antidiabetic, anti‐inflammatory activities compared their unfermented counterparts. At 30°C, most significant production had been discovered at 48 h incubation 2.5% rate inoculation culture in milks cow. Additionally, both...
This study explores the antidiabetic and anti-inflammatory activities of polypeptides from fermented whey protein isolate (WPI) soy (SPI) using Limosilactobacillus fermentum (KGL4) MTCC 25515. Both WPI SPI demonstrated significant α-amylase α-glucosidase inhibition, with showing higher inhibition rates. Proteolytic activity peaked at 48 h a 2.5% inoculation rate, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (PAGE) two-dimensional (2D)-PAGE revealing changes in profiles peptide...
Abstract Key steps of essential metabolic pathways are housed in plant peroxisomes. We conducted a microscopy-based screen for anomalous distribution peroxisomally targeted fluorescence Arabidopsis thaliana. This uncovered 34 novel alleles 15 genes affecting oil body mobilization, fatty acid β-oxidation, the glyoxylate cycle, peroxisome fission, and pexophagy. Partial loss-of-function lipid-mobilization enzymes conferred peroxisomes clustered around retained bodies without other notable...
Since their discovery over 5 decades ago, quinolone antibiotics have found enormous success as broad spectrum agents that exert activity through dual inhibition of bacterial DNA gyrase and topoisomerase IV. Increasing rates resistance, driven largely by target-based mutations in the GyrA/ParC resistance determining region, eroded utility threaten future use this vital class antibiotics. Herein we describe optimization a series 4-(aminomethyl)quinolin-2(1H)-ones, exemplified 34, inhibit IV...
Binding of arrestin to light-activated rhodopsin involves recognition the phosphorylated C-terminus and several residues on cytoplasmic surface receptor. These sites are in close proximity dark, unphosphorylated rhodopsin. To address position mobility active inactive receptor, we combined high-resolution solution solid state NMR spectroscopy intact mammalian photoreceptor detergent micelles as a function temperature. The 31P resonance by kinase at C-terminal tail was observable with single...
Herein, we describe the discovery and optimization of a novel series that inhibits bacterial DNA gyrase topoisomerase IV via binding to, stabilization of, cleavage complexes. Optimization this led to identification compound 25, which has potent activity against Gram-positive bacteria, favorable in vitro safety profile, excellent vivo pharmacokinetic properties. Compound 25 was found be efficacious fluoroquinolone-sensitive Staphylococcus aureus infection mouse thigh model at lower doses than...
It has long been known that medicinal plants are vital to human health and have used for therapeutic purposes since the beginning of civilization. The goal this study is evaluate scientific data supporting use herbal remedy Andrographis paniculata in management beta thalassemia. Phytocompounds, one bases traditional use, were assessed both qualitatively quantitatively five different extracts with varying solvent properties (methanol, chloroform, hexane, ethyl acetate, aqueous), providing...
It has long been known that medicinal plants are vital to human health and have used for therapeutic purposes since the beginning of civilization. The goal this study is evaluate scientific data supporting use herbal remedy Andrographis paniculata in management beta thalassemia. Phytocompounds, one bases traditional use, were assessed both qualitatively quantitatively five different extracts with varying solvent properties (methanol, chloroform, hexane, ethyl acetate, aqueous), providing...
A variety of metabolic pathways are sequestered in peroxisomes, conserved organelles that essential for human and plant survival. Peroxin (PEX) proteins generate maintain peroxisomes. The PEX1 ATPase facilitates recycling the peroxisome matrix protein receptor PEX5 is most commonly affected peroxin biogenesis disorders. Here, we describe isolation characterization of, to our knowledge, first Arabidopsis (Arabidopsis thaliana) pex1 missense alleles: pex1-2 pex1-3pex1-2 displayed...
Summary The single tapered dilator kit is the most commonly used percutaneous tracheostomy set in UK. Cook Blue Rhino™ and Portex Ultraperc™ were compared laboratory on mannequin porcine airway models. following data collected: subjective ease of dilating trachea inserting tube; time taken anterior–posterior compression during dilatation tube insertion; incidence extent posterior tracheal wall damage. During dilatation, caused less mean percentage (34.8% vs. 51.5%, p = 0.0014). There was no...
Comparative studies are useful in the prediction of validated 3D structure a query protein (Amylase). Modeler is used for amylase. All structural models verified by results ramachandran plot, Whatif server, Prosa (ll) and Procheck (Checks stereochemical quality structure, producing number postscript plots analyzing its overall residue-by- residue geometry. Predicted (after comparative study) based drug designing, protein-DNA interactions, study protein-protein interactions protein-ligand...
Background: Sickle cell disease (SCD) is a genetic disorder that affects the liver and other organs. Liver dysfunction common complication of SCD, but its prevalence characteristics are not well understood in Bilaspur population Chhattisgarh. Objective: To investigate SCD patients Bilaspur, Methods: This prospective cross-sectional study included 250 from function tests (LFTs) were performed to assess dysfunction. Results: The was high patients, with 60% having elevated enzymes. Aspartate...
Left heart disease is the most common cause of pulmonary hypertension (PH) and frequently accompanied by increases in vascular resistance. However, distinction between phenotypes PH due to left with a normal or elevated resistance-isolated postcapillary (IpcPH) combined pre- (CpcPH), respectively-has been incompletely defined using unbiased methods.