A novel severe acute respiratory syndrome human coronavirus (SARS HCoV) was identified from illness patients (named SARS-CoV-2 by ICTV) in December 2019 and has recently emerged as a serious threat to world public health.

Microbial ferulic acid decarboxylase (FADase) catalyzes the transformation of to 4-hydroxy-3-methoxystyrene (4-vinylguaiacol) via non-oxidative decarboxylation. Here we report crystal structures Enterobacter sp. Px6-4 FADase and enzyme in complex with substrate analogues. Our analyses revealed that possessed a half-opened bottom β-barrel catalytic pocket located between middle core helical bottom. Its structure shared high degree similarity members phenolic (PAD) superfamily. Structural...

Cuticle-degrading proteases are involved in the breakdown of cuticle/eggshells nematodes or insects, a hard physical barrier against fungal infections. Understanding 3-dimensional structures these proteins can provide crucial information for improving effectiveness fungi biocontrol applications, e.g., by targeted protein engineering. However, remain unknown. Here, we report two cuticledegrading from species nematophagous fungi. The were solved with X-ray crystallography to resolutions 1.65 Å...

Chitinases are a group of enzymes capable hydrolysing the β-(1,4)-glycosidic bonds chitin, an essential component fungal cell wall, shells nematode eggs, and arthropod exoskeletons. from pathogenic fungi have been shown to be putative virulence factors, can play important roles in infecting hosts. However, very limited information is available on structure chitinases nematophagous fungi. Here, we present 1.8 Å resolution first Family 18 chitinase this fungi, that Clonostachys rosea...

Introduction: Luteolin inhibits platelet activation and thrombus formation, but the mechanisms are unclear. This study investigated effects of luteolin on GPVI-mediated in vitro explored effect thrombosis, coagulation, production vivo. Methods: Washed human platelets were used for aggregation, membrane protein expression, ATP, Ca2+, LDH release, adhesion/spreading, clot retraction experiments. to detect collagen convulxin-induced reactive oxygen species endogenous antioxidant effects....

The low-carbon and sustainable operation challenges the wastewater treatment plant (WWTP), notably as influent temperatures vary seasonally. Optimizing operational parameters is a feasible approach, but current methods generally face large computational cost imprecise optimization. In response, this study developed novel seasonal multiobjective optimization method based on deep learning to trade-off effluent quality index (EQI), (OCI), greenhouse gas (GHG) emissions. crucial control...

Background Complex interconnections are evident among gut microbiota, circulating metabolites, inflammatory cytokines, and the pathogenesis of abdominal aortic aneurysms (AAA), with causal dynamics yet to be comprehensively elucidated. The primary objective this study was elucidate potential relationships involving microbiota-mediated plasma AAA.

Abstract Background Aortic dissection(AD) is an acute process of large blood vessels characterized by dangerous pathogenic conditions and high disability mortality. The pathogenesis AD remains debated. Matrix metalloproteinase-12 (MMP-12) participates in many pathological processes such as abdominal aortic aneurysm, atherosclerosis, emphysema cancer. However, this elastase has rarely been assessed the presence AD. aim present study was to investigate expression MMP-12 tissue so offer a...

The physicochemical bases for enzyme cold-adaptation remain elusive.

Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) restricts platelet activation via collagen receptor GPVI/FcRγ-chain. In this study, screening against collagen-induced aggregation was performed to identify functional CEACAM1 extracellular domain fragments. fragments, including Ala-substituted peptides, were synthesized. Platelet assays conducted on healthy donor samples for aggregation, cytotoxicity, adhesion, spreading, and secretion. Mice used tail bleeding FeCl3-induced...

Cuticle-degrading proteases secreted by nematophagous fungi can degrade nematode cuticle during infection. Alkaline from nematode-parasitic show stronger nematicidal activity in vitro than neutral nematode-trapping fungi. Sequence alignment of these revealed that the active-site residues were much conserved. Disulfide bridges alkaline not only contribute to thermal stability enzyme structure but also increase flexibility S1 and S4 pockets located at substrate-binding site. Molecular...

Circulating microRNAs (miRNAs) have emerged as potential biomarkers for cardiovascular diseases. However, few studies focused on the role of exosomal miRNAs in acute coronary syndrome (ACS). The purpose this study was to explore weather serum microRNA-146a (exo-miR-146a) could be used a novel diagnostic biomarker ACS and investigate its relationship with inflammatory response.A total 63 patients 25 normal arteries (Control) were enrolled respectively. exosomes isolated then identified by...

Pyrroline-5-carboxylate reductase (P5CR) encoded by PYCR1 gene is a housekeeping enzyme that catalyzes the reduction of P5C to proline using NAD(P)H as cofactor. In this study, we used in silico approaches examine role nonsynonymous single-nucleotide polymorphisms and their putative functions pathogenesis Cutis Laxa. Among 348 identified SNPs, 15 were predicted be potentially damaging both SIFT PolyPhen tools; them two SNP‐derived mutations, R119G G206W, have been previously reported...

Abstract: Atherosclerosis (AS) is the most common and serious complication in type 2 diabetes mellitus (T2DM). Recent studies have emphasized that inflammation main cause of atherosclerosis. Studies shown carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1) regulates expression matrix metallopeptidase 9 (MMP-9) after ischemic stroke to reduce inflammation. The aim this study was elucidate potential molecular mechanism CEACAM1 on inflammatory response serum levels CEACAM1,...

Pyrroline-5-carboxylate reductase (P5CR1) is a universal housekeeping enzyme that catalyzes the reduction of Δ1-pyrroline-5-carboxylate (P5C) to proline with concomitant oxidation NAD(P)H NAD(P) + . The enzymatic cycle between P5C and important for function in amino acid metabolism, apoptosis, intracellular redox potential balance mitochondria. Autosomal recessive cutis laxa (ARCL) results from mutation P5CR1 encoded by PYCR1. Specifically, R119G reported be linked ARCL although it has not...

Thermoactinospora rubra YIM 77501T is an aerobic, Gram-positive, spore-forming and cellulose degrading thermophilic actinomycete isolated from a sandy soil sample of volcano. Its growth temperature range 28-60°C. The genomic sequence this strain revealed that there are 27 cellulase genes belonging to six glycoside hydrolase families. To understand the strategy uses utilize carbon sources such as at different temperatures, comparative transcriptomics analysis T. was performed by growing it...

Matrix metalloproteinases-12 (MMP12) can lead to degradation of elastin resulting in plaque destabilization and rupture. MMP12 also facilitates platelet aggregation, adhesion, granule secretion. However, evidence the literature related function ST-segment elevation myocardial infarction (STEMI) is little. This study investigated expression human coronary thrombus examined relationship between plasma STEMI. Arterial was obtained from 46 STEMI patients 52 stable angina pectoris (SAP) 30...

The esterase Est8 from the thermophilic bacterium Bacillus sp. K91 belongs to GDSL family and is active on a variety of acetylated compounds, including 7-aminocephalosporanic acid. In contrast other esterases family, catalytic residues Asp182 His185 were more pivotal for activity than Ser11 residue. To better understand biochemical enzymatic properties Est8, recombinant protein was purified crystallized. Crystals obtained by hanging-drop vapour-diffusion method using 2.0 M ammonium sulfate,...

CrChi1 is a chitinase from the nematophagous fungus Clonostachys rosea that plays role in infection of nematodes. In order to resolve crystal structure and gain better understanding its biological functions, recombinant was crystallized at 291 K using PEG 3350 ammonium dihydrogen phosphate as precipitant 1.8 A resolution X-ray data set collected single flash-cooled (100 K). The crystals belonged space group P2(1), with unit-cell parameters = 44.1, b 71.7, c 59.1 A, alpha gamma 90, beta 91.3...