A5015362467- Connexins and lens biology
- Heat shock proteins research
- Nicotinic Acetylcholine Receptors Study
- Natural product bioactivities and synthesis
- Heme Oxygenase-1 and Carbon Monoxide
- Autophagy in Disease and Therapy
- Effects of Radiation Exposure
- Epigenetics and DNA Methylation
- Ferroptosis and cancer prognosis
- Cancer-related molecular mechanisms research
- Anesthesia and Sedative Agents
- Cancer, Stress, Anesthesia, and Immune Response
- Healthcare and Venom Research
- Photodynamic Therapy Research Studies
- Cancer Immunotherapy and Biomarkers
- Lung Cancer Treatments and Mutations
- Molecular Biology Techniques and Applications
- Ultrasound and Hyperthermia Applications
- MicroRNA in disease regulation
- Flavonoids in Medical Research
- Cancer-related Molecular Pathways
- Berberine and alkaloids research
- Cancer, Lipids, and Metabolism
- Cancer-related gene regulation
- Ion channel regulation and function
Fudan University Shanghai Cancer Center
2025
Shanghai Medical College of Fudan University
2025
Bengbu Medical College
2014-2023
Chengdu University of Traditional Chinese Medicine
2020
GTx (United States)
2019
Rutgers, The State University of New Jersey
2013-2015
Sun Yat-sen University
2009-2013
Rutgers New Jersey Medical School
2009-2013
Kaiping Central Hospital
1999
Harbin Medical University
1999
Procyanidins (PCs), which are organic antioxidants, suppress oxidative stress, exhibit anti-apoptotic properties, and chelate metal ions. The potential defense mechanism of PCs against cerebral ischemia/reperfusion injury (CIRI) was investigated in this study. Pre-administration for 7 days a PC enhanced nerve function decreased cerebellar infarct volume mouse middle artery embolization paradigm. In addition, mitochondrial ferroptosis enhanced, exhibited by shrinkage roundness, increased...
Cancer patients are often concurrently treated with analgesics and antineoplastic drugs, yet the influence of analgesic agents on therapeutic activity drugs is largely unexplored. This study investigates effects three commonly used analgesics, which produce analgesia by different mechanisms, cytotoxicity induced cisplatin, a widely antitumor agent, relation between those modulation gap junction function analgesics.The role junctions in cisplatin toxicity explored manipulation connexin...
Previous studies have shown that irinotecan (CPT‑11) impairs chemotherapy‑induced apoptosis by activating nuclear factor‑κB (NF‑κB) and a number of strategies been employed to augment chemosensitivity through the suppression NF‑κB activation. Berberine, botanical alkaloid, was reported enhance 5‑fluorouracil doxorubicin suppressing In present study, effect berberine on CPT‑11‑induced investigated inhibition NF‑κB. Inhibition activation p65 small interfering RNA potentiate induced CPT‑11....
Cisplatin [cis-diamminedichloroplatinum(II)]/oxaliplatin [1,2-diamminocyclohexane(trans-1)oxolatoplatinum(II)] toxicity is enhanced by functional gap junctions between treated cells, implying that inhibition of may decrease cytotoxic activity these platinum-based agents. This study investigates the effect junction modulation cisplatin/oxaliplatin on cytotoxicity in a transformed cell line. The effects were explored using junctional channels expressed transfected HeLa cells and purified...
Increasing gap junction activity in tumor cells provides a target by which to enhance antineoplastic therapies. Previously, several naturally occurring agents, including all-trans retinoic acid (ATRA) have been demonstrated increase junctional intercellular communication (GJIC) number of types cancer cells. In the present study, we investigated vitro whether ATRA modulates response human hepatocellular carcinoma (HCC) sorafenib, only proven oral drug for advanced HCC, and underlying...
Pannexin (Panx) plays a crucial role in several cellular processes such as immune cell death, proliferation, invasion, and migration, apoptosis, autophagy. However, the of Panx regulating migration invasion testicular cancer remains to be elucidated. In present study, we determined correlation between Panx-1 channel function I-10 cells. Transwell wound healing assays showed that inhibition by carbenoxolone (CBX) probenecid (PBN) attenuated cells vitro. Moreover, knockdown with short hairpin...
Colorectal cancer (CRC) is a highly malignant tumor associated with poor prognosis, yet the molecular mechanisms are not fully understood. In this study, we showed that LYAR, nucleolar protein, expressed at higher level in CRC tissue than adjacent normal and LYAR expression closely distant metastasis. only significantly promotes migration invasion of cells vitro, but knockdown (KD) also inhibits xenograft metastasis vivo. Microarray analysis KD combined chromatin immunoprecipitation (ChIP)...
Cysteine-scanning mutagenesis combined with thiol reagent modification is a powerful method which to define the pore-lining elements of channels and changes in structure that accompany channel gating. Using Xenopus laevis oocyte expression system two-electrode voltage clamp, we performed cysteine-scanning several residues connexin 26 (Cx26) hemichannels, followed by chemical using methanethiosulfonate (MTS) reagent, help identify position gate. Unexpectedly, observed effect MTS on currents...
CDGSH iron sulfur domain 2 can inhibit ferroptosis, which has been associated with cerebral ischemia/reperfusion, in individuals head and neck cancer. Therefore, may be implicated ischemia/reperfusion injury. To validate this hypothesis the present study, we established mouse models of occlusion middle artery HT22 cell oxygen-glucose deprivation reoxygenation to mimic injury vivo vitro, respectively. We found remarkably decreased expression brain tissue cells. When used adeno-associated...
Panax Notoginseng Saponins (PNS) have been well known to anti-tumor activity and enhance cytotoxicity of some cancer chemotherapy agents, but the mechanisms underlying these effects are still unknown. This study investigates effect PNS on cisplatin relationship between this modulation gap junctions (GJ) function by in a transfected cell line. The (0.25–1 µg/mL) was increased presence GJ. Inhibition junction either GJ blocker or interception Connexin (Cx) expression decreased cisplatin....
Oxaliplatin is widely used in the treatment of variety cancers, including cancer testis and colorectum. Gap junctions (GJs) can amplify cytotoxicity antinoeoplastic drugs through bystander effect different cells. In this study, we demonstrate that total flavonoids litsea coreana (TFLC), one extract from dried leaves leve, increase oxaliplatin mouse testicular I-10 We found cell survival was substantially decreased only when functional GJs formed TFLC increased cytotoxity (inducing death...
Gap junctions (GJs) serve the principal role in antineoplastic (cytotoxicity and induced apoptosis) effect of chemical drugs. The aim present study was to determine GJ intercellular communication (GJIC) composed connexin 43 (Cx43) on adriamycin cytotoxicity breast cancer cells. Four cell lines (Hs578T, MCF-7, MDA-MB-231 SK-BR-3) with different degree malignancy were used study. results western blotting immunofluorescence revealed that, Hs578T MCF-7 cells, which have a low malignancy,...
Pannexin1 (Panx-1) is a gap junction channel protein that mediates the release of intracellular ATP during autophagy, and thus plays an important role in tumor cell apoptosis chemo-resistance. However, Panx-1 cisplatin-resistance testicular cancer cells remains unclear. We found cisplatin-resistant I-10 lines (I-10/CDDP) autophagy-associated proteins (p62, p-mTOR, mTOR LC3) exhibited high levels autophagy their expression, while LC3-II expression was more significantly presence lysosomal...
In this study, we hypothesized that total flavonoid of Litsea coreana leve (TFLC) protects against focal cerebral ischemia/reperfusion injury. TFLC (25, 50, 100 mg/kg) was administered orally to a rat model injury, while the free radical scavenging agent, edaravone, used as positive control drug. Results neurological deficit scoring, 2,3,5-triphenyl tetrazolium chloride staining, hematoxylin-eosin staining and biochemical tests showed at different doses significantly alleviated...
Previously, Panax notoginseng saponin (PNS)-induced enhancement of gap junction (GJ) formation or function was observed to be responsible for the increased cytotoxic action cisplatin. PNS has three constituents, ginsenoside Rg1 and Rb1, notoginsenoside R1. The active compounds in enhancing cytotoxicity cisplatin remain unknown. Thus, effects main components on were investigated, as well correlation with modulation GJ transfected HeLa cells. (0.25-1 µg/ml) presence GJs. By contrast, decreased...