A5045796751- Reproductive Physiology in Livestock
- Reproductive System and Pregnancy
- Veterinary Equine Medical Research
- Endometriosis Research and Treatment
- Diverticular Disease and Complications
- Sperm and Testicular Function
- Animal health and immunology
- Infant Nutrition and Health
- Pregnancy and preeclampsia studies
- Urologic and reproductive health conditions
- Preterm Birth and Chorioamnionitis
- Galectins and Cancer Biology
- Circular RNAs in diseases
- Reproductive Biology and Fertility
- Kruppel-like factors research
- melanin and skin pigmentation
- Reproductive tract infections research
- Medicinal Plant Pharmacodynamics Research
- Actinomycetales infections and treatment
- T-cell and Retrovirus Studies
- Viral Infections and Vectors
- Click Chemistry and Applications
- Metal complexes synthesis and properties
- Pregnancy-related medical research
- Urinary Tract Infections Management
Colorado State University
2024-2025
University of Kentucky
2013-2024
Abstract Improved understanding of the molecular mechanisms underlying ascending equine placentitis holds potential for development new diagnostic tools and therapies to forestall placentitis-induced preterm labor. The current study characterized placental transcriptome (chorioallantois [CA] endometrium [EN]) during (placentitis group, n = 6) in comparison gestationally-matched controls (control 6). Transcriptome analysis identified 2953 805 differentially expressed genes CA EN placentitis,...
Abstract Problem Ascending placentitis is one of the leading causes abortion in horse. Minimal work has focused on its effect fetal fluids or antenatal immune response fetus. Methodology Placentitis was induced via transcervical inoculation Streptococcus equi ssp Zooepidemicus , and fluids/serum/tissues were collected 4‐6 days later following euthanasia. Cytokine concentrations detected using a multiplex immunoassay within (amniotic allantoic) serum (maternal fetal) inoculated control mares....
Abstract Problem Ascending placentitis is the leading cause of abortion in horse. Interleukin (IL)‐6 considered predictive placental infection other species, but little understood regarding its role pathophysiology ascending placentitis. Method study Sub‐acute was induced via trans‐cervical inoculation S zooepidemicus , and various fluids/serum/tissues collected 8 days later. Concentrations IL‐6 were detected within fetal fluids serum inoculated (n = 6) control mares. RNASeq performed on...
Abstract Background Equine embryonic loss following the development of endometrial cups delays return to cyclicity due production equine chorionic gonadotropin (eCG). Natural degradation coincides with an influx immune cells at 100–120 days gestation, but therapeutic stimulation reduced eCG has been relatively unsuccessful. Recently, we observed increase in pro‐inflammatory cytokine use immunostimulant mycobacterium cell wall fraction (MCWF). Objectives To evaluate efficacy...
Nocardioform placentitis (NP) continues to result in episodic outbreaks of abortion and preterm birth mares remains a poorly understood disease. The objective this study was characterize the transcriptome chorioallantois (CA) with NP. CA were collected from confirmed NP based upon histopathology, microbiological culture PCR for Amycolatopsis spp. Samples margin lesion (NPL, n = 4) grossly normal region (NPN, 4). Additionally, samples postpartum (Control; CRL, Transcriptome analysis...
ABSTRACT Introduction The interleukin (IL)‐6 family of cytokines is grouped by a common receptor subunit (gp130), but functions in distinct overlapping physiological activities, including regulation acute phase reaction and the balance between effector regulatory T cell populations—both which play role successful pregnancy maturation. Methods Here, we aim to assess expression profiles members IL‐6 cytokine throughout equine gestation. To do so, RNA Sequencing was performed on chorioallantois...
Abstract Problem Progestins are immunomodulatory in a variety of species. In the horse, most commonly administered synthetic progestin is altrenogest (ALT), but its effect on immune system non‐pregnant mare unknown. Methods Peripheral blood mononuclear cells (PBMCs) from diestrous mares were incubated with varying concentrations progesterone (P4) or ALT to assess intracellular production IFNγ and expression select cytokines. Additionally, ten received either VEH daily utilizing switchback...
Sperm-neutrophil binding is an important facet of breeding and significantly impacts fertility. While a specific seminal plasma protein has been found to reduce this improve fertility (CRISP-3), additional molecule(s) appear promote between defective sperm neutrophils. Recent work suggested one these proteins lactoferrin (LF), 80 kDa iron-binding throughout the body, but purity was not confirmed. It unknown if LF binds selectively based on viability, receptors for are located equine sperm....