Background: The current study aimed to investigate the impact of asymptomatic or mild severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection on female fertility and laboratory clinical outcomes in assisted reproductive technology (ART) treatments.Methods: Patients undergoing ART treatments Reproductive Medicine Center, Tongji Hospital, Wuhan, from May 2020 February 2021 were enrolled. Seventy them positive for serum SARS-CoV-2 antibodies (IgG and/or IgM), 3973 patients had...

This study aimed to evaluate the influences of SARS-CoV-2 infection on semen parameters and investigate impact in vitro fertilization (IVF) outcomes.This retrospective enrolled couples undergoing IVF cycles between May 2020 February 2021 at Tongji Hospital, Wuhan. Baseline characteristics were matched using propensity score matching. Participants categorized into an unexposed group (SARS-COV-2 negative) exposed positive) based a history infection, populations 148 50 after matching,...

ABSTRACT Background Ovarian tumor metastasis to the stomach is uncommon. Clinical manifestations of are variable and lack features. Gastric resembling a subepithelial unusual, making diagnosis challenging. Case Presentation Here, we reported case gastric from ovarian adenocarcinoma presenting as diagnosed by endoscopic ultrasound‐guided tissue acquisition. Conclusion This provides significant reference value serves cautionary reminder for management tumors.

This study aimed to identify genetic causes responsible for multiple morphological abnormalities of the sperm flagella (MMAF) in Han Chinese population. Three primary infertile males with completely immobile and MMAF were enrolled. Whole-exome sequencing Sanger performed disease-causing genes. Subsequently, ultrastructural analyses investigated. The probable impact variants on protein function was analyzed by online bioinformatic tools immunofluorescence assay. patients dynein axonemal heavy...

To minimize the rate of in vitro fertilization (IVF)- associated multiple-embryo gestation, significant efforts have been made. Previous studies related to machine learning IVF mainly focused on selecting top-quality embryos improve outcomes, however, patients with sub-optimal prognosis or medium- inferior-quality embryos, selection between SET and DET could be perplexing.This was an application study including 9211 10,076 treated during 2016 2018, Tongji Hospital, Wuhan, China. A...

Abstract Background Increasing evidence has shown that the mammalian target of rapamycin (mTOR) pathway plays a critical role in oocyte meiosis and embryonic development, however, previous studies reporting effects on IVM showed different or even opposite results, specific mechanisms were not clear. Methods The immature oocytes from female mice underwent with at concentrations to select an optimal dose. maturation rate, activation subsequent cleavage blastocyst formation rates, spindle...

To improve the developmental competence of in vitro cultured oocytes, extensive literature focused on maturation rate improvement with different additives culture medium, while studies investigating dynamics oocytes during (IVM) and influencing factors oocyte viability are scarce.The study involved a retrospective observation by time-lapse monitoring IVM process 157 donated GV from 59 infertile couples receiving ICSI 2019, Tongji Hospital, Wuhan, China. The derived controlled ovarian...

This study aimed to evaluate the influences of SARS-CoV-2 vaccination (CoronaVac) on male fertility and investigate impact a history CoronaVac in males gamete embryo development vitro fertilization (IVF) outcomes.A prospective cohort enrolled couples undergoing IVF cycles between June August 2021 at Reproductive Medicine Center, Tongji Hospital, Medical College, Huazhong University Science Technology China. According males, participants were divided into group non-vaccination group. A...

We studied the influence of various parameters high-frequency nanosecond pulse bursts on strength rabbit muscle contractions. Ten unipolar with field intensities E (1 kV/cm, 4 and 8 kV/cm), intraburst frequencies f (10 kHz, 100 1 MHz), numbers N (1, 10, 100) were applied using a pair plate electrodes to surface skin rabbits’ biceps femoris, acceleration signal contraction near electrode was measured 3-axis sensor. A time- frequency-domain analysis signals showed that peak value increases...

Women over age 35 suffer from the inadequate number and poor quality of oocytes during assisted reproductive treatment, making full use by in vitro maturation (IVM) is crucial. Rapamycin could improve developmental competences post-maturation aging, yet its effects on IVM process an aged population were not clear. In this study, immature mice or older women underwent with without 10 nM rapamycin, followed parthenogenetic activation insemination embryo culture. The competence both groups...

The appearance of smooth endoplasmic reticulum aggregation (SERa) is one the most common dysmorphic phenotypes oocytes, however, impact SERa occurrence on in vitro fertilization (IVF) outcomes controversial. This study aimed to investigate oocytes aneuploidy subsequent embryos IVF.In this retrospective cohort study, a total 114 intracytoplasmic sperm injection (ICSI) cycles with undergoing preimplantation genetic testing for (PGT-A) were enrolled, and among them there 323 SERa(+) 1253...

Can rapamycin improve the developmental competence of human oocytes during IVM process?Rapamycin at 10 nM could markedly undergoing IVM.Embryos derived from that mature in vitro have lower than sibling embryos matured vivo. Rapamycin was shown to effectively outcomes mammalian oocytes; however, its effects on not been investigated.In 2021, donated immature (n = 202) 80 infertile couples receiving ICSI were included a control group, and 156 72 group. The underwent with or without (control)...

Abstract Using 70 U/ml or 35 as CA125 routine abnormal threshold may result in omissions the relapse detection of Ovarian cancer (OvCa). This study aimed to clarify association between a biochemical (only elevation CA125) and an image-identified predict relapsed lesions better. 162 patients who achieved complete clinical response were enrolled from women diagnosed with stage I-IV serous ovarian, tubal, peritoneal cancers January 2013 June 2019 at our center. The level 2 × nadir was defined...

Fallopian tube cancer that is characterized only by inguinal lymph node metastasis without intra-abdominal widespread rare. Here we report a patient with primary bilateral metastases as the first symptom.

To estimate the effects of early cervical lesions (ECL) on female reproductive function and IVF/ICSI cycle outcomes, a retrospective cohort study involving 111 infertile women from 2014 to 2019 was performed. Thirty-seven with history ECL seventy-four controls, undergoing cycles, were included in group comparison respectively. Demographic characteristics, ovarian reserve, outcomes both groups collected. Basal serum FSH level, AMH AFC, number oocytes retrieved matured, normal fertilization...

Rationale: Sepsis is a common stressor that may decrease microcirculation in the gastrointestinal tract patients and increase bleeding risk of stress-related mucosal disease. However, CRUSADE (Can Rapid Risk Stratification Unstable Angina Patients Suppress Adverse Outcomes with Early Implementation ACC/AHA Guidelines) score, recommended by authoritative guidelines for acute coronary syndrome (ACS), does not include sepsis as factor. Patient concerns: The 2 cases were about ACS hemorrhagic...

Multi-walled carbon nanotubes with excellent electrical properties and high aspect ratios can reduce the field strength required to kill cancer cells in vitro nanosecond pulsed electric fields. For first time, this article systematically comprehensively evaluates effects of various parameters fields combined multi-walled on cell viability. The strength, E (2-10 kV/cm); pulse width, τ (100-500 ns); number, N (5-260) viability A375 human skin presence are studied using Cell Counting Kit 8...