A5052030516- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Renal and related cancers
- Hormonal and reproductive studies
- Sexual function and dysfunction studies
- Ovarian function and disorders
- Reproductive Health and Technologies
- Cancer-related molecular mechanisms research
- Cancer-related Molecular Pathways
- Hippo pathway signaling and YAP/TAZ
- Assisted Reproductive Technology and Twin Pregnancy
- Cystic Fibrosis Research Advances
- Molecular Biology Techniques and Applications
- Cancer Cells and Metastasis
- Prenatal Screening and Diagnostics
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- Urinary Bladder and Prostate Research
- PI3K/AKT/mTOR signaling in cancer
Tongji Hospital
2022-2023
Huazhong University of Science and Technology
2022-2023
This study aimed to identify genetic causes responsible for multiple morphological abnormalities of the sperm flagella (MMAF) in Han Chinese population. Three primary infertile males with completely immobile and MMAF were enrolled. Whole-exome sequencing Sanger performed disease-causing genes. Subsequently, ultrastructural analyses investigated. The probable impact variants on protein function was analyzed by online bioinformatic tools immunofluorescence assay. patients dynein axonemal heavy...
Background Psychological stress and its two response systems, the hypothalamic-pituitary-adrenal (HPA) axis autonomic nervous system (ANS), are closely related to psychogenic erectile dysfunction (pED). However, analyses of perceived systems in pED patients need be more in-depth, especially interactions between them. Methods Our study included 75 with pEDs healthy men. The International Index Erectile Function-5 (IIEF-5) 10-item Perceived Stress Scale (PSS-10) were used for assessing...
Multiple elements in human sperm have been demonstrated to play significant roles the reproductive process, but their simultaneous detection single cells remains challenging. We propose a novel analytical procedure using single-cell inductively coupled plasma-time of flight-mass spectrometry (scICP-TOF-MS) simultaneously quantify multiple individual cells. A promising label-free cell identification strategy based on endogenous element was developed obtain valid data. The contents exhibited...
Mental stress and imbalance of its two neural systems, the autonomic nervous system (ANS) hypothalamic-pituitary-adrenal (HPA) axis, are associated with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) erectile dysfunction (ED). However, comprehensive analyses psychological systems under-investigated, particularly in CP/CPPS patients complicated by lower urinary tract symptoms (LUTS) ED. Participants were 95 CP/CPPS+ED group, 290 124 ED group 52 healthy men control group. The...
Metformin, the first-line oral antidiabetic medicine, has shown great antineoplastic potential in various cancer types, despite an unclear mechanism. This study aimed to elucidate possible mechanism of metformin as a chemotherapy agent with less reproductive and genetic toxicity human endometrial cancer. The type I carcinoma cell lines Ishikawa RL95-2 were treated metformin. Cell functions, such proliferation, migration, invasion, analyzed. Flow cytometry was performed for cycle apoptosis...
Background: Cervical cancer is the fourth leading cause of mortality among gynecological malignancies. However, changes in multicellular ecosystems between cervical premalignant and malignant lesions remain unclear.Methods: We performed single-cell mRNA sequencing on ~122,400 cells from 20 biopsies, including 5 healthy controls, 4 high-grade intraepithelial neoplasias, microinvasive carcinomas cervix, 6 invasive squamous carcinomas.Findings: identified stem by high enhancer zeste 2 polycomb...
Abstract Background: To compare the efficacy and safety of follitropin delta in its individualized fixed-dose regimen with alfa a conventional adjustable dosing Chinese women. Methods: This was subgroup analysis randomized, multi-center, assessor-blind, non-inferiority trial (GRAPE) including 759 women (aged 20-40 years) recruited 16 reproductive medicine clinics China. Women were randomized 1:1 ratio to be treated either dosed based on anti-Müllerian hormone (AMH) body weight or following...