A5012250595- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Bio-sensing Technologies
- 3D Printing in Biomedical Research
- Electrowetting and Microfluidic Technologies
- Cellular Mechanics and Interactions
- Bone Tissue Engineering Materials
- Soft Robotics and Applications
- Advanced Biosensing Techniques and Applications
- Micro and Nano Robotics
- Additive Manufacturing and 3D Printing Technologies
- Biosensors and Analytical Detection
- Tissue Engineering and Regenerative Medicine
- Microbial Fuel Cells and Bioremediation
- Electrospun Nanofibers in Biomedical Applications
- Nonlinear Optical Materials Studies
- Supercapacitor Materials and Fabrication
- Electrochemical sensors and biosensors
- Mechanical and Optical Resonators
- Manufacturing Process and Optimization
- Surface Modification and Superhydrophobicity
- Cardiovascular and Diving-Related Complications
- Neuroscience and Neural Engineering
- Advanced Materials and Mechanics
- Pickering emulsions and particle stabilization
University of Maryland, College Park
2015-2024
University of California, Berkeley
2009-2015
Berkeley Geochronology Center
2014-2015
Berkeley College
2014
The miniaturization of integrated fluidic processors affords extensive benefits for chemical and biological fields, yet traditional, monolithic methods microfabrication present numerous obstacles the scaling operators. Recently, researchers have investigated use additive manufacturing or "three-dimensional (3D) printing" technologies - predominantly stereolithography as a promising alternative construction submillimeter-scale components. One challenge, however, is that current lack ability...
Point-of-care (POC) and disposable biomedical applications demand low-power microfluidic systems with pumping components that provide controlled pressure sources. Unfortunately, external pumps have hindered the implementation of such due to limitations associated portability power requirements. Here, we propose demonstrate a 'finger-powered' integrated system as modular element head for variety advanced applications, including finger-powered on-chip microdroplet generation. By utilizing...
Microinjection protocols are ubiquitous throughout biomedical fields, with hollow microneedle arrays (MNAs) offering distinctive benefits in both research and clinical settings. Unfortunately, manufacturing-associated barriers remain a critical impediment to emerging applications that demand high-density of hollow, high-aspect-ratio microneedles. To address such challenges, here, hybrid additive manufacturing approach combines digital light processing (DLP) 3D printing "ex situ direct laser writing (
Diverse cellular processes are influenced by the mechanical properties of substrate. Here we introduce methodology constructing micropost array gradients to investigate effects unidirectional substrate stiffness cues on living cells. Experimental results revealed preferential cell migration in direction increasing stiffness.
"Multi-stage" fluidic reactions are integral to diverse biochemical assays; however, such processes typically require laborious and time-intensive mixing procedures in which distinct reagents and/or washes must be loaded sequentially separately (i.e., one-at-a-time). Microfluidic processors that enable multi-stage with suspended microparticles (e.g., microbeads cells) performed autonomously could greatly extend the efficacy of lab-on-a-chip technologies. Here we present a single-layer...
Direct laser writing (DLW) is a three-dimensional (3D) manufacturing technology that offers significant geometric versatility at submicron length scales. Although these characteristics hold promise for fields including organ modeling and microfluidic processing, difficulties associated with facilitating the macro-to-micro interfaces required fluid delivery have limited utility of DLW such applications. To overcome this issue, here we report an in-situ (isDLW) strategy creating 3D...
A microfluidic system has been designed and constructed by means of micromachining processes to integrate both mixing mobile microbeads hydrodynamic microbead arraying capabilities on a single chip simultaneously detect multiple bio-molecules. The prototype four parallel reaction chambers, which include microchannels 18 × 50 µm2 cross-sectional area section 22 cm length. Parallel detection DNA oligonucleotide sequences was achieved via molecular beacon probes immobilized polystyrene 16 µm...
Precision hydrodynamic controls of microparticles (e.g., microbeads and cells) are critical to diverse lab-on-a-chip applications. Microfluidic particulate-based arraying techniques widely used; however, achieving full microarray resettability without sacrificing trapping performance has remained a significant challenge. Here we present single-layer methodology for releasing high numbers after microfluidic process. Experiments with suspended streptavidin-coated polystyrene (15 μm in...
A microfluidic bead-based diode that uses a targeted circular-shaped microchannel for docking suspended microbead is presented rectifying fluid flow under low Reynolds number conditions.
We present a continuous flow microfluidic “rail-trap-and-rail” reactor for passively: (i) executing multi-stage particulate-based mixing processes, and (ii) arraying select particles at each reaction stage.
A wide range of emerging biomedical applications and clinical interventions rely on the ability to deliver living cells via hollow, high-aspect-ratio microneedles. Recently, microneedle arrays (MNA) have gained increasing interest due inherent benefits for drug delivery; however, studies exploring potential harness such advantages cell delivery been impeded difficulties in manufacturing MNAs suitable delivering mammalian cells. To bypass these challenges, here we leverage extend our...
A wide range of endovascular interventions rely on surgical tools such as guidewire-catheter systems for navigating through blood vessels to, example, deliver embolic materials, stents, and/or therapeutic agents to target sites well biopsy (e.g., forceps and punch needles) medical diagnostics. In response the difficulties in maneuvering instruments safely effectively access intended body, researchers have developed an array soft robotic that harness fluidic pneumatic or hydraulic) actuation...
Microfabrication processes enable the biophysical control of cellular environments at micro- and nanoscale. The mechanical properties arrayed microposts have been demonstrated to influence diverse functions including cell motility, yet response changes in micropost spacing remains unclear. In this work, a microfabricated post array with variable stiffness was constructed investigate effects these factors on motility. Over length 675 µm, between decreased from 6 2 µm single direction,...
Patent Ductus Arteriosus (PDA) is a heart condition in which the ductus arteriosus-a blood vessel connecting pulmonary artery to aorta fetus-fails undergo closure after birth. A PDA can be an important factor neonates born with severe congenital disease (CHD) or prematurely. With advent of new intravascular stent technologies, treatments based on arteriosus stenting now completed many cases; however, difficulties remain accessing small babies successfully using current guidewire-catheter...
Abstract Among the wide range of additive manufacturing — or “three-dimensional (3D) printing” technologies, “material jetting” approaches are distinctively suited for multi-material fabrication. Because material jetting strategies, such as “PolyJet 3D printing”, harness inkjets that allow multiple photopolymer droplets (and sacrificial support materials) to be dispensed in parallel build objects, distinct materials with unique properties can readily unified a single print akin combining...
In this paper, we present a versatile `human-powered' microfluidic system that encapsulates microbeads within droplets to perform medical diagnostics assay. Point-of-care (POC) devices hold great promise for medicinal applications throughout the world. particular, portable, low-cost systems can be operated by non-medical personnel without electrical supplies are desired. Here PDMS device (2.8 cm × 1.9 0.8 cm) achieve four distinct accomplishments: (i) pressing force from single human finger...
Here we present and demonstrate the concept of a versatile `human-powered' fluid pumps as modular element to provide pressure head for variety microfluidic systems. Several distinctive accomplishments have been achieved: (1) human finger pumping actuation force; (2) without using any electricity, (3) integrated pump with passive safety valve two one-way valves, (4) successful demonstration in delivering fluids/particles into chip. For first time, demonstrated that measured by was between...
Microenvironmental biophysical stimuli influence diverse cellular functions, such as directional motility and stem cell differentiation. Previously, researchers have tuned the linear stiffness of microposts to investigate mechanobiological processes direct behavior; however, suffer from an inherent, yet critical drawback - regulation micropost is fundamentally limited "biaxial" control. To overcome this issue, here we utilize three-dimensional (3D) direct-write laser lithography fabricate...
Autonomous fluidic components are critical to the advancement of integrated micro/nanofluidic circuitry for lab-on-a-chip applications, such as point-of-care (POC) molecular diagnostics and on-site chemical detection. Previously, a wide range self-regulating microfluidic components, diodes, have been developed; however, achieving effective functionality at ultra-low Reynolds number (e.g., Re <; 0.05) has remained significant challenge. To overcome this issue, here we introduce single-layer...
The ability to achieve multi-layer synthesis on the surface of microbeads is critical for diverse chemical and biological assays. Although microfluidic techniques layer-by-layer (LbL) have been demonstrated droplets, accomplishing continuous flow has remained a significant challenge. Here we present micropost array railing (μPAR) system LbL functionalization microbead substrates in device. Microposts arrayed at 1° angle served as directing into adjacent streams bio-molecules washes. An...
Microfluidic components that are capable of autonomous "on-chip" operations at ultra-low Reynolds number (e.g., Re <; 0.2) critical to the advancement integrated fluidic circuitry for chemical and biological applications, including point-of-care (POC) molecular diagnostics on-site detection. Previously, researchers have utilized dynamic resistive elements, such as suspended microbeads rotational microstructures, rectify 0.2 flow; however, systems require hydrodynamic forces return elements...
Passive fluidic components that are capable of rectifying fluid flow at ultra-low Reynolds Number critical to the advancement micro/nanofluidic circuitry for diverse chemical and biological applications, such as sample preparation on chip, molecular diagnostics, point-of-care (POC) testing, quantitative cell biology platforms. Previously, a wide range diodic have been developed rectify in systems; however, engineering microfluidic diodes function flows (i.e., Re <; 0.25) emerging platforms...
This paper reports the first demonstration of single nucleotide polymorphism (SNP) genotyping via molecular beacon probes immobilized on polystyrene microbead substrates within a dynamic microfluidic system. Additionally, we present an optimized bead immobilization technique, micropost array trapping (muPAT), for high-density and high-throughput arraying beads. Quantitative detection was achieved at room temperature three label-free DNA oligonucleotide sequences based genome Hepatitis C...