A5034024641- CRISPR and Genetic Engineering
- Genetically Modified Organisms Research
- Insect Resistance and Genetics
- Advanced biosensing and bioanalysis techniques
- Animal Genetics and Reproduction
- Molecular Biology Techniques and Applications
- Identification and Quantification in Food
- Biosensors and Analytical Detection
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Lipid metabolism and biosynthesis
- Intellectual Property and Patents
- Plant Reproductive Biology
- Lung Cancer Treatments and Mutations
- ATP Synthase and ATPases Research
- Metabolism and Genetic Disorders
- Plant Genetic and Mutation Studies
- Nitrogen and Sulfur Effects on Brassica
- Cancer Genomics and Diagnostics
- Mitochondrial Function and Pathology
- DNA Repair Mechanisms
- Plant biochemistry and biosynthesis
- Chromosomal and Genetic Variations
- CCD and CMOS Imaging Sensors
- Advanced Nanomaterials in Catalysis
Oil Crops Research Institute
2015-2024
Chinese Academy of Agricultural Sciences
2015-2024
Ministry of Agriculture and Rural Affairs
2020-2024
Beijing Chest Hospital
2021-2023
Capital Medical University
2021-2023
Farmland Irrigation Research Institute
2022
Yuan Ze University
2018
Lanzhou Institute of Husbandry and Pharmaceutical Sciences
2014
Gansu Agricultural University
2014
AU Optronics (Taiwan)
2010
Plant non-specific lipid transfer proteins (nsLTPs) constitute large multigene families that possess complex physiological functions, many of which remain unclear. This study isolated and characterized the function a protein gene, BraLTP1 from Brassica rapa, in important oilseed crops napus. encodes predicted secretory protein, little known VI Class nsLTP families. Overexpression BnaLTP1 B. napus caused abnormal green coloration reduced wax deposition on leaves detailed analysis revealed...
Five previously reported Brassica napus endogenous reference genes, including acetyl-CoA carboxylase gene (BnACCg8), phosphoenolpyruvate (PEP), oleoyl hydrolase (FatA), high-mobility-group protein I/Y (HMG-I/Y) and cruciferin A (CruA), were analyzed for their PCR specificity between B. other species the quantification stability among different cultivars. sequencing results indicated that none of these systems was species-specific as required by genetically modified organism labeling policy....
Abstract The Cauliflower mosaic virus (CaMV) 35S promoter (P35S) is a commonly used target for detection of genetically modified organisms (GMOs). There are currently 24 reported methods, targeting different regions the P35S promoter. Initial assessment revealed that due to absence primer binding sites in sequence, 19 methods failed detect MON88913 cotton and other two could only be applied certain GMOs. rest three were not suitable measurement some testing events, because SNPs primer/probe...
Organic-inorganic hybrid perovskites (OIHPs) have aroused widespread interest because of their exceptional performance in thermal, optical, electric, and information storage applications. However, it is relatively rare to respond both...
Abstract Background The upgrading of rapeseed cultivars has resulted in a substantial improvement yield and quality China over the past 30 years. With selective pressure against fatty acid composition oil content, high erucic acid- low oil-content have been replaced by cultivars. cultivar Zhongyou 821 its descendent, Zhongshuang 9, are representatives two generations most outstanding Chinese ( B. napus ) developed 2 decades. This paper compares transcriptional profiles 9 for 32 genes that...
Abstract The rapid increase in the number of genetically modified (GM) varieties has led to a demand for high-throughput methods detect organisms (GMOs). We describe new dynamic array-based high throughput method simultaneously 48 targets samples on Fludigm system. test included species-specific genes, common screening elements, most Chinese-approved GM events and several unapproved events. TaqMan assays successfully amplified products from both single-event complex with GMO DNA amount 0.05...
Genome-edited plants created by genome editing technology have been approved for commercialization. Due to molecular characteristics that differ from classic genetically modified organisms (GMOs), establishing regulation-compliant analytical methods identification and quantification of genome-edited has always regarded as a challenging task. An editing-site-specific PCR method was developed based on the unique edited sequence in CAO1-edited rice plants. Test results seven primer/probe sets...
The genetically modified (GM) maize DBN9936 with a biosafety certificate will soon undergo commercial application. To monitor the safety of maize, three genomic DNA (gDNA) reference materials (RMs) (DBN9936a, DBN9936b, and DBN9936c) were prepared nominal copy number ratios 100%, 3%, 1% for event, respectively. DBN9936a was from leaf tissue gDNA homozygotes, while DBN9936b DBN9936c by quantitative mixing tissues homozygotes non-GM counterparts. Validated DBN9936/zSSIIb duplex droplet digital...
Except for the events RT73, MS8, RF3, and T45, event-specific detection methods most commercialized genetically modified (GM) rapeseed varieties have not been established, as a result, enforcement of organism labeling policies has hindered. The rapeseeds, MS1×RF1 MS1×RF2, are 2 11 approved GM-rapeseed commercialization. In this study, right border junction fragments between gene construct genome RF1, RF2, MS1 were isolated using commercially available GenomeWalker technology. Homology...
Transgenic rice TT51–1 (BT63) is an insect resistant strain that was granted for safety certificate in China 2009. This study characterizes the transgenic event using a GenomeWalker strategy. The organization of transgenes indicated on two plasmids, pFHBT1 and pGL2RC7, had been integrated at same locus. sequence spanned 8725 bp, including truncated Cry1Ab/Ac cassette, intact Amp gene segments, Hph segment. 5′ 3′ plant flanking sequences were isolated used to locate chromosome 10 TT51–1....
The third-generation epidermal growth factor receptor (EGFR) -tyrosine kinase inhibitor (TKIs), such as osimertinib, designed for targeting the acquired drug-resistant mutation of EGFR T790M, was approved first-line therapy advanced EGFR-mutated non-small cell lung cancer (NSCLC). Thus, detection T790M NSCLC is crucial. However, tissue samples are often difficult to obtain, especially in patients at stages. This study assessed performances droplet digital polymerase chain reaction (ddPCR)...
The practice of blending cheap palm oil with more expensive oils is currently rampant owing to the increased global price and gap between types oils. This adulteration poses a serious threat trade edible negatively affects consumers. aim this study was identify presence as an additive in using PCR-based technique. A taxon-specific gene, MT3-B, found by searching GenBank database. MT3-B showed high (Elaeis guineensis Jacq.) specificity, low intraspecies variability, copy number. On basis...