A5032682300- Cancer Research and Treatments
- Extracellular vesicles in disease
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Nanoplatforms for cancer theranostics
- Amino Acid Enzymes and Metabolism
- MicroRNA in disease regulation
- Radiation Therapy and Dosimetry
- Cancer, Hypoxia, and Metabolism
- Diet and metabolism studies
- Virus-based gene therapy research
- Tea Polyphenols and Effects
- Advanced biosensing and bioanalysis techniques
- Advanced Radiotherapy Techniques
- Cancer Cells and Metastasis
- Nanoparticle-Based Drug Delivery
- Antimicrobial Resistance in Staphylococcus
- Analytical chemistry methods development
- PARP inhibition in cancer therapy
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer, Lipids, and Metabolism
- Biochemical and Structural Characterization
- Polymer Surface Interaction Studies
- Gold and Silver Nanoparticles Synthesis and Applications
- Trace Elements in Health
Kobe University
2009-2025
AntiCancer (United States)
2020-2023
University of California, San Diego
2020-2023
Kobe University Hospital
2018-2020
Swedish Medical Center
2020
Johns Hopkins University
2020
American Association of Physicists in Medicine
2020
Ashland (United States)
2020
Philips (Finland)
2020
John Wiley & Sons (United States)
2020
Small extracellular vesicles (sEVs) are reliable biomarkers for early cancer detection; however, conventional detection methods such as immune-based assays and microRNA analyses not very sensitive require sample pretreatments long analysis time. Here, we developed a molecular imprinting-based dynamic molding approach to fabricate antibody-conjugated signaling nanocavities capable of size recognition. This enabled the establishment an easy-to-use, rapid, sensitive, pretreatment-free,...
Background/Aim: Exosomes are produced by normal and cancer cells. found in the serum of patients have been used for diagnosis prognosis. Recently tears from non-cancer to contain exosomes. In present report we describe advanced breast-cancer patients. Materials Methods: We oncogenic miRNAs exosomes isolated tear fluids obtained five with metastatic breast compared them form eight healthy volunteers. Results: Tear had a significantly higher quantity exosome markers than (CD9, CD63). were...
Methionine addiction, found in all types of cancer investigated, is because the overuse methionine by cells for excess transmethylation reactions. In present study, we compared histone H3 lysine-methylation status and degree malignancy between methionine-addicted their isogenic methionine-independent revertants, selected growth low concentration methionine. The revertans can grow on levels or independently exogenous using precursors, as do normal cells. trimethylated lysine marks parental...
Methionine addiction, a fundamental and general hallmark of cancer, known as the Hoffman Effect, is due to altered use methionine for increased aberrant transmethylation reactions. However, linkage addiction malignancy cancer cells incompletely understood. An isogenic pair methionine-addicted parental osteosarcoma their rare methionine-independent revertant enabled us compare them malignancy, epithelial-mesenchymal phenotype, pattern histone-H3 lysine-methylation. Methionine-independent 143B...
Abstract Exosomes are small (30–100 nm) membrane vesicles that serve as regulatory agents for intercellular communication in cancers. Currently, exosomes detected by immuno‐based assays with appropriate pretreatments like ultracentrifugation and time consuming (>12 h). We present a novel pretreatment‐free fluorescence‐based sensing platform intact exosomes, wherein exchangeable antibodies fluorescent reporter molecules were aligned inside exosome‐binding cavities. Such antibody‐containing...
Methionine addiction is a fundamental and general hallmark of cancer, termed the Hoffman effect. due to excessive use dependence on methionine by cancer cells. In present report, we correlated extent degree malignancy with amount stability methylated histone H3 lysine marks.We established low- high-malignancy variants from parental human pancreatic-cancer cell line compared their sensitivity restriction methylation status.A low-malignancy, low-methionine-addiction revertant had less H3K9me3...
The precise mechanism of intercellular communication between cancer cells following radiation exposure is unclear. Exosomes are membrane‑enclosed small vesicles comprising lipid bilayers and mediators that transport a variety intracellular components, including microRNAs (miRNAs or miRs). present study aimed to identify novel roles exosomes released from irradiated neighboring cells. In order confirm the presence in human pancreatic cell line MIAPaCa‑2, ultracentrifugation was performed...
Background/Aim: Methionine addiction, a fundamental and general hallmark of cancer, is due to the excess use methionine for transmethylation, described as Hoffman-effect. Methionine-addicted cancer cells can revert at low frequency independence when selected under methionine-restriction. We report here that highly-malignant methionine-addicted H460 human lung-cancer cells, independence, have greatly-reduced tumorigenic potential. Materials Methods: parental methionine-independent revertant...
Triple-negative breast cancer (TNBC) is a recalcitrant disease. The present study examined the efficacy of methionine restriction and poly ADP-ribose polymerase (PARP)-inhibitor olaparib on BRCA1/2 wild-type BRCA1 mutated TNBC cell lines. human line MDA-MB-231, BRCA1-mutant lines MDA-MB-436 HCC1937 were used to examine sensitivity recombinant methioninase (rMETase) or methionine-free medium combination olaparib. Cell viability was using WST-8 reagent as well by direct counting after Hoechst...
Abstract E6‐associated protein (E6AP) is a cellular ubiquitin ligase that mediates ubiquitylation and degradation of p53 in conjunction with the high‐risk human papillomavirus E6 proteins. However, physiological functions E6AP are poorly understood. To identify novel biological function E6AP, we screened for binding partners using GST pull‐down mass spectrometry. Here identified annexin A1, member superfamily, as an E6AP‐binding protein. Ectopic expression enhanced A1 vivo. RNAi‐mediated...
In our previous study, monogalactosyl diacylglycerol (MGDG) purified from spinach was found to have cytotoxic effects in human cancer cell lines. This study further assessed whether MGDG can enhance the of radiation pancreatic cells vitro and vivo.Glycoglycerolipids including were extracted dried spinach. The cytotoxicity evaluated by MTT assay using four lines (MIAPaCa-2, AsPC-1, BxPC-3 PANC-1) normal dermal fibroblasts (NHDFs). alone or combination MIAPaCa-2 analyzed with colony forming...
Background/Aim: Sarcomas are considered a heterogeneous disease with incomplete understanding of its molecular basis. In the present study, to further understand general changes in sarcoma, patient-derived xenograft (PDX) mouse models three most common soft-tissue sarcomas: myxofibrosarcoma, undifferentiated pleomorphic sarcoma (UPS) and liposarcoma were established methylation status histone H3 lysine marks was studied. Materials Methods: Immunoblotting immunohistochemical staining used...
Background/Aim: Exosomes are extracellular vesicles produced by both normal and cancer cells. Previous research has demonstrated that circulating exosomes derived from cells may create a niche for future metastasis, distant the primary tumor. In present report, were captured quantified based on exosome-surface proteins in pre- post-operative serum of breast patients, focusing exosome markers CD9 CD63, as well HER2, therapeutic target cancer. Materials Methods: Eight patients recruited, their...
Hepatitis C virus (HCV) is the major causative agent of hepatocellular carcinoma. However, precise mechanism underlying carcinogenesis yet to be elucidated. It has recently been reported that Syk, a non-receptor protein tyrosine kinase, functions as potent tumour suppressor in human breast This study first examined possible effect HCV infection on expression Syk vivo . Immunohistochemical analysis revealed endogenous which otherwise was expressed diffusely cytoplasm normal hepatocytes,...
obesity is a world-wide recalcitrant problem leading to many diseases. Dietary methionine restriction (MR) has been shown prevent obesity, but it an onerous regimen. The present study aimed determine the effects of oral recombinant methionase (o-rMETase), on preventing in mice high-fat diet.Male C57BL/6J control group were fed diet (CD) (+6.5% fat) for 25 days, and others (HF) (+34.3% days. Then, divided into three dietary groups: 1) HF + phosphate buffered saline (PBS) group; 2) o-rMETase...
Objectives Methionine addiction is a fundamental and general hallmark of cancer caused by enhanced methyl flux. In the present study, we effected novel methionine-methylation blockade to target patient-derived orthotopic xenograft model pancreatic cancer. Methods The mouse models were randomized into 6 groups 8 mice each treated for 2 weeks: untreated control; azacitidine; oral recombinant methioninase (o-rMETase); o-rMETase plus cycloleucine; cycloleucine azacitidine (triple-methyl...
The high incidence and mortality of cancer make it a global health issue. However, conventional therapies have several disadvantages, especially serious side effects due to low selective toxicity cells. Gold nanoparticles (AuNPs) are an excellent drug carrier, enhance delivery efficiency, hold promise for photothermal radiation therapies. (−)-Epigallocatechin-3-gallate (EGCG) is the major polyphenolic antioxidant constituent green tea, has potent antitumor effect, binds specifically 67 kDa...
Metal nanoparticles (NPs), such as gold NPs (AuNPs), are particularly sensitive to X-rays, and thus specific accumulation of AuNPs in a tumor would allow radiotherapy with low energy X-rays reduced side effects. can be generated using HAuCl4 the natural polyphenol epigallocatechin-3-gallate (EGCG) presence citrate. Here, we EGCG-AuNPs several additives examined these mouse tumors following intravenous administration. 15 nm diameter sodium alginate accumulated more compared 40-nm-diameter...
<h3>Abstract</h3> Background/Aim: Methionine addiction is the elevated requirement for exogenous methionine growth and survival of cancer cells, termed Hoffman effect. Methionine-addicted cells synthesize normal or excess amounts but still need an external source methionine. restriction (MR) by either a methionine-free medium in vivo low-methionine diet methioninase, selectively arrests late S/G<sub>2</sub> cell cycle phase, not cells. The present study focuses on comparison production...
Background/Aim: BRCA1/2 mutations in breast cancer cells impair homologous recombination and promote alternative end joining (Alt-EJ) for DNA-damage repair. DNA polymerase theta, encoded by POLQ, plays a crucial role Alt-EJ, making it potential therapeutic target, particularly BRCA1/2-mutant cancers. Methionine restriction is promising approach to target due their addiction this amino acid. The present study investigated the expression of POLQ wild-type BRCA1-mutant under methionine...
Accumulating lines of evidence indicate that the N-terminal domain prion protein (PrP) is involved in susceptibility mice. In this study, to investigate role octapeptide repeat (OR) region alone for and pathogenesis disease, we intracerebrally inoculated RML scrapie prions into tg(PrPΔOR)/Prnp0/0 mice, which express mouse PrP missing only OR on PrP-null background. Incubation times these mice were not extended. Protease-resistant PrPΔOR, or PrPScΔOR, was easily detectable but lower brains...