A5015136567- Adipose Tissue and Metabolism
- Lipid metabolism and biosynthesis
- Muscle metabolism and nutrition
- Research in Cotton Cultivation
- Cardiac, Anesthesia and Surgical Outcomes
- Inflammatory Bowel Disease
- Immune Response and Inflammation
- Microbial Metabolic Engineering and Bioproduction
- Hemoglobinopathies and Related Disorders
- Receptor Mechanisms and Signaling
- Neonatal Respiratory Health Research
- Pancreatic function and diabetes
- Hormonal and reproductive studies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Mitochondrial Function and Pathology
- Venous Thromboembolism Diagnosis and Management
- IL-33, ST2, and ILC Pathways
- Cell Adhesion Molecules Research
- Anesthesia and Neurotoxicity Research
- Immune Cell Function and Interaction
- Pharmaceutical studies and practices
- Antibiotics Pharmacokinetics and Efficacy
- Cardiac Ischemia and Reperfusion
- Exercise and Physiological Responses
- Iron Metabolism and Disorders
Leiden University Medical Center
2022-2025
Geneeskundige en Gezondheidsdienst
2023
St. Antonius Ziekenhuis
2018-2022
Eurofins (Netherlands)
2022
Karolinska Institutet
2014-2021
Maastricht University
2012-2020
Maastricht University Medical Centre
2011-2016
University Medical Center
2013-2015
Columbia University
2014
Wageningen University & Research
2014
Perilipin 5 (PLIN5/OXPAT) is a lipid droplet (LD) coat protein mainly present in tissues with high fat-oxidative capacity, suggesting role for PLIN5 facilitating fatty acid oxidation. Here, we investigated the of fat oxidation skeletal muscle. In human muscle, observed that (but not PLIN2) content correlated tightly OXPHOS and rat muscle mitochondrial respiration rates on lipid-derived substrate. This prompted us to examine expression mitochondria by means immunogold electron microscopy...
Type 2 diabetes is characterized by excessive lipid storage in skeletal muscle. Excessive intramyocellular (IMCL) exceeds intracellular needs and induces lipotoxic events, ultimately contributing to the development of insulin resistance. Lipid droplet (LD)-coating proteins may control proper Perilipin (PLIN2/adipose differentiation-related protein [ADRP]) one most abundantly expressed LD-coating Here we examined role PLIN2 myocellular handling sensitivity investigating effects vitro...
IBD (inflammatory bowel disease), where CD (Crohn's disease) and UC (ulcerative colitis) represent the two main forms, are chronic inflammatory conditions of intestine. Macrophages play a central role in pathogenesis regulated by major differentiation factors such as CSF-1 (colony-stimulating factor 1) homoeostasis inflammation. IL (interleukin)-34 has recently been discovered second ligand for CSF-1R (CSF-1 receptor). However, expression involvement IL-34 remain unknown. In present paper,...
FNDC4 is a secreted factor sharing high homology with the exercise-associated myokine irisin (FNDC5). Here we report that Fndc4 robustly upregulated in several mouse models of inflammation as well human inflammatory conditions. Specifically, levels are increased locally at inflamed sites intestine bowel disease patients. Interestingly, administration recombinant model induced colitis markedly reduces severity compared mice injected control protein. Conversely, lacking develop more severe...
A low fat oxidative capacity has been linked to muscle diacylglycerol (DAG) accumulation and insulin resistance. Alternatively, a oxidation rate may stimulate glucose oxidation, thereby enhancing disposal. Here, we investigated whether an ethyl-2-[6-(4-chlorophenoxy)hexyl]-oxirane-2-carboxylate (etomoxir)-induced inhibition of leads storage An intervention in healthy male subjects was combined with studies human primary myotubes. Furthermore, DAG triacylglycerol (TAG), mitochondrial...
Abstract The proper functional interaction between different tissues represents a key component in systemic metabolic control. Indeed, disruption of endocrine inter-tissue communication is hallmark severe dysfunction obesity and diabetes. Here, we show that the FNDC4-GPR116, liver-white adipose tissue axis controls glucose homeostasis. We found liver primarily controlled circulating levels soluble FNDC4 (sFNDC4) lowering hepatokine led to prediabetes mice. Further, identified orphan adhesion...
In contrast to insulin-resistant individuals, insulin-sensitive athletes possess high intramyocellular lipid content (IMCL), good mitochondrial function and perilipin 5 (PLIN5) levels, suggesting a role for PLIN5 in benign IMCL storage. We hypothesised modulating fasting-mediated insulin resistance. Twelve men were fasted 60 h, before after which muscle biopsies taken stained droplets (LDs), laminin. Confocal microscopy images analysed LD size, number, association subcellular distribution....
Patients with COVID-19-associated pulmonary embolism have been reported to require higher doses of unfractionated heparin (UFH) achieve therapeutic activated partial thromboplastin time (APTT) levels. This study aimed compare the UFH dose in ICU patients COVID-19 and control patients, exploring possible explanatory factors. In this retrospective cohort at Leiden University Medical Center, 162 (admitted between 15 March 2020 1 January 2022) 1006 from 2014 2020) were included. All had an...
Abstract Laboratory medicine is faced with rapid developments in data exchange, secondary use of and artificial intelligence. Safe exchange laboratory requires a suitable terminology standard. NPU, LOINC SNOMED CT are increasingly used for this purpose, but none these standards can currently accommodate safe across the full spectrum conventional data. Furthermore, technological advances in, amongst others, ‘omics’ area will enforce shift towards precision diagnostics. These emerging...
Background . Fibronectin type III domain-containing ( FNDC ) proteins fulfill manifold functions in tissue development and regulation of cellular metabolism. FNDC4 was described as anti-inflammatory factor, upregulated inflammatory bowel disease (IBD). signaling includes direct cell-cell interaction well release bioactive peptides, like shown for or FNDC5 The G-protein-coupled receptor 116 GPR116 found a putative receptor. We here aim to comprehensively analyze the mRNA expression FNDC1 ,...
Quantitative, label-free coherent Raman microscopy was used to show lipid droplet compositional differences in muscle upon plin5 overexpression<italic>in vivo</italic>and<italic>in vitro</italic>.
Dissipating energy via mitochondrial uncoupling has been suggested to contribute enhanced insulin sensitivity. We hypothesised that skeletal muscle mitochondria of endurance-trained athletes have increased sensitivity for fatty acid (FA)-induced uncoupling, which is driven by the protein adenine nucleotide translocase 1 (ANT1). Capacity FA-induced was measured in male (T) and sedentary young men (UT) an observational study also isolated from Zucker diabetic (ZDF) rats C2C12 myotubes...
Abstract Aims/hypothesis Physical inactivity, low mitochondrial function, increased intramyocellular lipid (IMCL) deposition and reduced insulin sensitivity are common denominators of chronic metabolic disorders, like obesity type 2 diabetes. Yet, whether function predisposes to resistance in humans is still unknown. Methods Here we investigated, an intervention study, muscle with oxidative capacity, induced by one-legged physical would feature stronger signs lipid-induced resistance. To...
Altered skeletal muscle lipid metabolism is a hallmark feature of type 2 diabetes (T2D). We investigated turnover in T2D versus BMI-matched control subjects (controls) and examined whether putative vivo differences would be preserved the myotubes. Male obese individuals (n = 6) controls underwent hyperinsulinemic-euglycemic clamp, VO2max test, dual-energy X-ray absorptiometry scan, underwater weighing, biopsy vastus lateralis. (14)C-palmitate (14)C-oleate oxidation rates incorporation into...
BACKGROUND: Acute kidney injury (AKI) is associated with mortality after cardiac surgery. Novel risk factors may improve identification of patients at for renal injury. The authors evaluated the association between preoperative biomarkers that reflect cardiac, inflammatory, renal, and metabolic disorders surgery–associated AKI (CSA-AKI) in elderly patients. METHODS: This was a secondary analysis 2-center prospective cohort study “Anesthesia Geriatric Evaluation.” Twelve were determined...
Differential intestinal expression of the macrophage growth factors colony stimulating factor-1 (CSF-1), interleukin (IL)-34, and their shared CSF-1 receptor (CSF-1R) in inflammatory bowel disease (IBD) has been shown. Diverse between IL-34, suggest that IL-34 may signal via an alternate receptor. Receptor-type protein-tyrosine phosphatase ζ (PTPRZ1, RPTP-ζ), additional receptor, was recently identified. Here, we aimed to assess PTPRZ1 IBD non-IBD biopsies. Further, investigate cellular...