The indiscriminate use of deltamethrin in agriculture and aquaculture can lead to residues increased many regions, which poses negative impacts on intestinal health aquatic organisms. Although the potential toxicity have recently attracted attention, comprehensive studies injuries after chronic exposure remain poorly understood. Herein, a 28-day test, crucian carp expose different concentrations (0, 0.3, 0.6 μg/L) were used as research object. We found that morphology changes goblet cells...

To investigate whether Mandarin fish developed oxidative stress after being domesticated with artificial feed, we conducted a series of experiments. Oxidative is an important factor leading to diseases and aging in the body. The liver integrates functions such as digestion, metabolism, detoxification, coagulation, immune regulation, while gills are respiratory organs that sensitive changes water environment. Therefore, used research materials. aim this study was effects short-term feed...

More and more evidences proved that deltamethrin (Del) exposure induced adverse effects damaged immune function to the aquatic animals in parasite killing process with increasing insecticide application. However, little is currently known of negative effect on mucosal immunity, especially gills tissue. Therefore, this study was aimed reveal tissue injury immunotoxicity gill gibel carp following acute exposure. The LC50 at 96 h determined be 6.194 μg/L, then juvenile (Carassius auratus...

Niclosamide (NIC) is the only commercially available molluscicide for controlling schistosomiasis, and its negative effects on aquatic animals had been frequently reported in recent years. However, toxicity mechanism of NIC Chinese soft-shelled turtle (Pelodiscus sinensis) have not yet investigated. Therefore, juvenile turtles were exposed to 0 (control group), 10 (low NIC, L), 50 (high H) μg/L 120 h our results demonstrated that exposure induced severe pathological changes liver P....

Abstract Stress is an important contributing factor in the outbreak of infectious fish diseases. To comprehensively understand impact catecholamine stress hormone norepinephrine (NE) on pathogenicity Aeromonas hydrophila , we assessed variations bacterial growth, virulence‐related genes expression and virulence factors activity after NE addition serum‐SAPI medium. Further, effects A. vivo by challenging with pathogenic strain AH196 following or without injection. The NE‐associated...

Aeromonas hydrophila can be a substantial concern, as it causes various diseases in aquaculture. An effective and green method for inhibiting A. is urgently required. Emodin, naturally occurring anthraquinone compound, was exploited photo-antimicrobial agent against hydrophila. At the minimum inhibitory concentration of emodin (256 mg/L) to inactivate hydrophilia 30 min, an 11.32% survival rate observed under 45 W white compact fluorescent light irradiation. In addition, antibacterial...

Abstract Background Classification of the Acanthocephala, a clade obligate endoparasites, remains unresolved because insufficiently strong resolution morphological characters and scarcity molecular data with sufficient resolution. Mitochondrial genomes may be suitable candidate, but they are available for small number species their suitability task has not been tested thoroughly. Methods Herein, we sequenced first mitogenome large family Rhadinorhynchidae: Micracanthorhynchina dakusuiensis ....

Abstract Background Acanthocephala is a clade of obligate endoparasites whose mitochondrial genomes (mitogenomes) and evolution remain relatively poorly understood. Previous studies reported that atp8 lacking from acanthocephalan mitogenomes, tRNA genes often have nonstandard structures. Heterosentis pseudobagri (Arhythmacanthidae) an fish endoparasite for which no molecular data are currently available, biological information unavailable in the English language. Furthermore, there...

Current treatment strategies for parasitic infectious diseases in crucian carp primarily rely on chemotherapy. As a commonly used antiparasitic agent, deltamethrin (DEL) may have the potential adverse effects external mucosa of fish such as gills. In this study, 180 healthy juvenile (Carassius auratus) (average weight: 8.8 ± 1.0 g) were randomly divided into three groups 28 days, which immersed 0 μg/L, 0.3 and 0.6 μg/L DEL, respectively. The results histological analysis revealed that severe...

Netting, loading and transporting can induce stress in fish, often resulting death subsequent economic losses to the aquaculture industry. Coilia nasus is strongly responsive stressors, thus this species presents a suitable model for research of response. In study, we investigated whether hypothalamus–pituitary–interrenal axis genes were involved responses transport C. nasus. mRNA expression profiles corticotropin-releasing hormone (CRH), urotensin I (UI) pro-opiomelanocortin (POMC) brain...

Niclosamide (NIC) is a potent salicylanilide molluscicide/helminthicide commonly utilized for parasite and mollusc control in aquatic environments. Due to its persistent presence water bodies, there growing concern regarding impact on organisms, yet this remains inadequately elucidated. Consequently, study aims assess the hepatotoxic effects detoxification capacity of black carp (

Leptin, a secretory protein encoded by obese genes, plays an important role in regulating feeding and energy metabolism fish. To study the structure function of Leptin gene yellow cheek carp (Elopichthys bambusa), full-length cDNA sequence leptin was cloned, named EbLep. The Eblep 1140 bp, length open reading frame (ORF), which can encode 174 amino acids, 525 bp. signal peptide predicted to contain 33 acids. Sequence alignment showed that acid conserved cyprinid Despite large differences...

Azygia hwangtsiyui (Trematoda, Azygiidae), a neglected parasite of predatory fishes, is little-known in terms its molecular epidemiology, population ecology and phylogenetic study. In the present study, complete mitochondrial genome A. was sequenced characterized: it 13,973 bp circular DNA molecule encodes 36 genes (12 protein-coding genes, 22 transfer RNA two ribosomal genes) as well non-coding regions. The A+T content mitogenome 59.6% displays remarkable bias nucleotide composition with...

Abstract Deltamethrin (Del), a widely administered pyrethroid insecticide, has been established as common contaminant of the freshwater environment and detected in many ecosystems. In this study, we investigated changes brain transcriptome metabolome crucian carp after exposure to 0.6 μg/L Del for 28 days. Elevated MDA levels inhibition SOD activity indicate damage antioxidant system. Moreover, total 70 differential metabolites (DMs) were identified using liquid chromatography‐mass...

Niclosamide (NIC, 2′,5-dichloro-4′-nitrosalicylanilide) is a salicylanilide molluscicide, and the extensive utilization environmental pollution associated with NIC engender potential hazard to both human health wellbeing of aquatic organisms. However, mechanism chronic toxicity at environmentally relevant concentrations in terms oxidative stress, metabolic disorder, barrier functions black carp (Mylopharyngodon piceus) unknown. Therefore, healthy juvenile (M. (average weight: 38.2 ± 2.5 g)...

Abstract Vibrio vulnificus is a pathogenic bacterium that causes disease in marine fish, affecting fish farming and human health worldwide. In May 2021, the Bohai Bay region, broke out commercially farmed pearl gentian grouper (♀ Epinephelus fuscoguttatus × ♂ lanceolatus ), causing huge economic losses. The diseased had skin lesions, water accumulation their abdomens, showed tissue organ damage. V. biotype 2 has been reported eels other but it less grouper. this study, strain isolated from...

Abstract Background Targeted next-generation sequencing (tNGS) has emerged as an efficient diagnostic method for pathogens identification. herein, we aimed to evaluate its performance in pathogen detection bronchoalveolar lavage fluid (BALF). Methods BALF samples were obtained from 262 adult patients with pulmonary infection and detected by tNGS, microbial culture, Xpert® MTB/RIF assay, Aspergillus galactomannan (GM) test. Results In total, 47 potential identified the including 21 bacteria,...

Calmodulin (Calm), a crucial Ca2+ sensor, plays an important role in calcium-dependent signal transduction cascades. However, the expression and relevance of Calm stress immune response have not been characterized Megalobrama amblycephala. In this study, we identified full-length cDNA (termed MaCalm) blunt snout bream M. amblycephala, analyzed MaCalm patterns to cadmium Aeromonas hydrophila challenges. was 1603 bp long, including 5′-terminal untranslated region (UTR) 97 bp, 3′-terminal UTR...

Niclosamide (NIC) is a commonly used insecticide and molluscicide in the prevention treatment of parasitic diseases fish. The utilization NIC has potential to disrupt microbial community present on mucosal tissue fish, leading localized inflammatory responses. objective this study was evaluate impact immune system bacterial populations within gill gut Mylopharyngodon piceus. Fish were subjected varying concentrations NIC, including control group (0 μg/L), low (15% 96 h LC50, LNG, 9.8 high...

Parasitic infestations present significant threats to the physiological health and ecological stability of aquatic species, frequently compromising immune defenses elevating mortality rates. This study was conducted elucidate non-specific responses induced by Pallisentis (Neosentis) celatus infection in Monopterus albus, with a focus on intestinal histopathology transcriptome gene expression. A histopathological examination revealed minor alterations villi under low-level infection....