A5033313702- Exercise and Physiological Responses
- Adipose Tissue and Metabolism
- Heat shock proteins research
- Muscle metabolism and nutrition
- Cardiovascular and exercise physiology
- Cancer survivorship and care
- Muscle Physiology and Disorders
- Systemic Sclerosis and Related Diseases
- Epigenetics and DNA Methylation
- Thermoregulation and physiological responses
- Telomeres, Telomerase, and Senescence
- Moringa oleifera research and applications
- Hormonal and reproductive studies
- Biochemical effects in animals
- Extracellular vesicles in disease
- Redox biology and oxidative stress
- Circadian rhythm and melatonin
- Dermatologic Treatments and Research
- Genetics and Physical Performance
- Mast cells and histamine
- Nutrition and Health in Aging
- Vitamin C and Antioxidants Research
- Childhood Cancer Survivors' Quality of Life
- Genetics, Aging, and Longevity in Model Organisms
- Circular RNAs in diseases
Foro Italico University of Rome
2016-2025
Centre Hospitalier Universitaire de Nice
2019
Sapienza University of Rome
2012-2015
Roma Tre University
2005
We describe a method for subcellular fractionation of mouse skeletal muscle, myoblast and myotubes to obtain relatively pure fractions nuclear, cytosolic mitochondrial compartments. Fractionation allows the analysis protein interest (or other cellular component) based on its compartmental distribution can also generate molecular information about state cell and/or tissue how may differ between different compartments, tissues or types, in response treatments ageing.The described was...
Platelet-rich plasma (PRP) has received increasing interest in applied medicine, being widely used clinical practice with the aim of stimulating tissue healing. Despite reported success, there is still a lack knowledge when considering biological mechanisms at base activity PRP during process muscle The present study was to verify whether local delivery modulates specific molecular events involved early stages regeneration process. right flexor sublimis anesthetized Wistar rats mechanically...
Although several studies have shown that immune cells stimulated by in vitro stress are capable to produce neurotrophins, there is still no evidence whether physiological stress, such as exercise, can modulate the vivo levels of brain-derived neurotrophic factor (BDNF) peripheral blood mononuclear (PBMCs).This work investigated acute exercise modulates expression BDNF, pro-BDNF, and p75(NTR) PBMCs 10 healthy young men who performed a cycling incremental test exhaustion (MAX) or exercised at...
Regular physical activity is effective as prevention and treatment for different chronic conditions related to the ageing processes. In fact, a sedentary lifestyle has been linked worsening of cellular biomarkers such telomere length (TL) and/or specific epigenetic changes (e.g. DNA methylation), with increase propensity aging-related diseases premature death. Extending our previous findings, we aimed test hypothesis that 12 weeks low frequency, moderate intensity, explosive-type resistance...
Oxidative stress represents a significant contributing factor to male infertility and sperm dysfunction. In this condition, an increase in ROS production exceeds the body's antioxidant defences, resulting decline spermatozoa quality fertilising capacity. Furthermore, excessive has been linked promotion of genomic damage, lipid peroxidation, inflammation, altered enzyme activity, ultimately, irreversible alterations, cell death, seminal parameters associated with infertility. It is...
We recently demonstrated that low frequency, moderate intensity, explosive-type resistance training (EMRT) is highly beneficial in elderly subjects towards muscle strength and power, with a systemic adaptive response of anti-oxidant stress-induced markers. In the present study, we aimed to evaluate impact EMRT on oxidative stress biomarkers induced old people (70–75 years) by single bout acute, intense exercise. Sixteen randomly assigned either control, not exercising group (n=8) or trained...
Objective. Hyperglycemia leads to increased production of reactive oxygen species (ROS) in type 2 diabetes, which reduces cellular antioxidant defenses and induces DNA lesions. The aim this study was investigate the effects on redox homeostasis oxidative damage exercise training patients with diabetes compared nondiabetic individuals. Methods Results. 12 sedentary diabetic males (62.1 ± 4.3 yrs) healthy (61.7 3.9 were exposed 4-month moderate training, 3 times per week, evaluate effect...
The imbalance between reactive oxygen species (ROS) production and antioxidant defense systems leads to macromolecule tissue damage as a result of cellular oxidative stress. This phenomenon is considered key factor in fatigue muscle following chronic or high-intensity physical exercise. In the present study, effect Moringa oleifera leaf extract (MOLE) was evaluated C2C12 myotubes exposed an elevated hydrogen peroxide (H2O2) insult. capacity influence myotube redox status through analysis...
Most anticancer treatments act on oxidative-stress pathways by producing reactive oxygen species (ROS) to kill cancer cells, commonly resulting in consequential drug-induced systemic cytotoxicity. Physical activity (PA) has arisen as an integrative therapy, having positive health effects, including redox-homeostasis. Here, we investigated the impact of online supervised PA program promoter-specific DNA methylation, and corresponding gene expression/activity, 3 antioxidants- (SOD1, SOD2, CAT)...
Abstract Thioredoxin reductase 1 (TrxR1) is a selenocysteine-containing protein involved in cellular redox homeostasis which downregulated skeletal muscle differentiation. Here we show that TrxR1 decrease occurring during myogenesis functionally the coordination of this process. Indeed, depletion reduces myoblasts growth by inducing an early -related gene expression pattern includes myogenin and Myf5 up-regulation Cyclin D1 decrease. On contrary, overexpression differentiation delays...
Physical activity has been demonstrated to be effective in the prevention and treatment of different chronic conditions, including type 2 diabetes (T2D). In particular, several studies highlighted how beneficial effects physical may related stability DNA molecule, such as longer telomeric ends. Here we analyze effect exercise training on telomere length, spontaneous H2O2-induced damage, well apoptosis level leukocytes from untrained or trained T2D patients vs. age-matched control subjects...
Moringa oleifera is a multi-purpose herbal plant with numerous health benefits. In skeletal muscle cells, leaf extract (MOLE) acts by increasing the oxidative metabolism through SIRT1-PPARα pathway. SIRT1, besides being critical energy sensor, involved in activation related to redox homeostasis of transcription factors such as nuclear factor erythroid 2-related (Nrf2). The aim present study was evaluate vitro capacity MOLE influence status C2C12 myotubes modulation total antioxidant (TAC),...
Besides its substantial role in eye lens, αB-crystallin (HSPB5) retains fundamental function striated muscle during physiological or pathological modifications. In this study, we aimed to analyse the cellular and molecular factors driving functional response of HSPB5 protein different muscles from mice subjected an acute bout non-damaging endurance exercise C2C12 myocytes upon exposure pro-oxidant environment, chosen as “in vivo” vitro” models a stressing conditions, respectively. To end,...
The COVID-19 pandemic has posed several challenges for the oncology health care system. need to improve patients’ Quality of Life (QoL) through exercise, which is related survival and healing, increased, especially during lockdowns. Technologies are often used help with patient as well monitor exercise training. This case study, developed period, aims evaluate effectiveness a proposed home-based combined training (CT) regimen, supervised online lessons, in increasing QoL fatigue breast...
Oxidative stress linked to vascular damage plays an important role in the pathogenesis of systemic sclerosis (SSc). Indeed, at nailfold capillaroscopy patients with Raynaud’s Phenomenon (RP) is a major risk factor for development SSc together presence specific autoantiobodies. Here, we investigated effects phosphodiesterase type 5 inhibitor (PDE5i) sildenafil, currently used management RP, modulating proinflammatory response dermal fibroblasts oxidative vitro. Human isolated from and healthy...
Regular physical activity can enhance immune function and effectively prevents the spread of cytokine response, thus reducing systemic low‐grade inflammation improving various markers. Moreover, regular exercise maintains redox homeostasis in skeletal muscle other tissues, including cells, but interconnection between anti‐inflammatory effects with status cells is still poorly understood. With aim to verify overall beneficial effect training on system, we have examined acute short‐term a...