A5038013237- RNA modifications and cancer
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Psoriasis: Treatment and Pathogenesis
- Cancer-related molecular mechanisms research
- Genetic Mapping and Diversity in Plants and Animals
- Single-cell and spatial transcriptomics
- Adipokines, Inflammation, and Metabolic Diseases
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- Antibiotic Resistance in Bacteria
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Regulation of Appetite and Obesity
- Genetics and Neurodevelopmental Disorders
- Pharmaceutical studies and practices
- Sexual Differentiation and Disorders
- Urinary Tract Infections Management
- Cancer-related gene regulation
- Metabolism, Diabetes, and Cancer
- Animal Genetics and Reproduction
- Listeria monocytogenes in Food Safety
- interferon and immune responses
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Cerebral Palsy and Movement Disorders
University of California, Riverside
2024-2025
Kumamoto University
2024
Kyushu University
2018-2022
Yamaguchi University
2022
The University of Tokyo
2018
Systems Biology Institute
2018
Kobe University
2015-2018
Adipocyte differentiation is regulated by various mechanisms, of which mitotic clonal expansion (MCE) a key step. Although this process known to be cell cycle modulators, the precise mechanism remains unclear. N6-Methyladenosine (m6A) posttranscriptional RNA modification, whose methylation and demethylation are performed respective enzyme molecules, has recently been suggested involved in regulation adipogenesis. Here, we show that an N6-adenosine methyltransferase complex consisting Wilms'...
Abstract Alternative pre-mRNA splicing (AS) is a fundamental regulatory process that generates transcript diversity and cell type variation. We developed Shiba, comprehensive method integrates assembly, event identification, read counting, differential analysis across RNA-seq platforms. Shiba excels in capturing annotated unannotated AS events with superior accuracy, sensitivity, reproducibility. It addresses the often-overlooked issue of junction imbalance, significantly reducing false...
Stable reference genes are important for gene expression analyses such as quantitative PCR. The stability of 15 candidate that can be used to developing mouse gonads was thoroughly verified using combinations multiple algorithms. these fluctuated greatly depending on the analysis period and/or gender. Peptidylprolyl isomerase A (Ppia) and polymerase (RNA) II (DNA directed) polypeptide (Polr2a) were stably a wide in gonads. Furthermore, stable corresponding gender ranked. These results useful...
Lipodystrophy and obesity are associated with insulin resistance metabolic syndrome accompanied by fat tissue dysregulation. Here, we show that serine protease inhibitor A1 (SerpinA1) expression in the liver is increased during recovery from lipodystrophy caused adipocyte-specific loss of signaling mice. SerpinA1 induces proliferation white brown preadipocytes increases uncoupling protein 1 (UCP1) to promote mitochondrial activation mature adipocytes. Liver-specific transgenic mice exhibit...
Mammalian sexual fate is determined by the presence or absence of sex determining region Y chromosome (Sry) in "bipotential" gonads. Recent studies have demonstrated that both male and female development are induced distinct active genetic pathways. Breeding from Mus m. domesticus poschiavinus (POS) strains into C57BL/6J (B6J) mice (B6J-XYPOS) has been shown to induce reversal (75%: bilateral ovary, 25%: true hermaphrodites). However, our B6N-XYPOS mice, which were generated backcrossing...
Psoriasis is a chronic inflammatory skin disease, for which genome-wide association studies (GWAS) have identified many genetic variants as risk markers. However, the details of underlying molecular mechanisms, especially are functional, poorly understood. We utilized computational approach to survey psoriasis-associated functional that might affect protein functions or gene expression levels. developed pipeline by integrating publicly available datasets provided GWAS Catalog, FANTOM5, GTEx,...
Genomic variations are associated with gene expression levels, which called quantitative trait loci (eQTL). Most eQTL may affect the total levels by regulating transcriptional activities of a specific promoter. However, direct exploration genomic promoter using RNA-seq data has been challenging because analyses treat estimated summing those all isoforms transcribed from distinct promoters. Here we propose new method for identifying activities, usage (puQTL), conventional data. By leveraging...
Abstract Alternative pre-mRNA splicing (AS) is a fundamental regulatory process that generates transcript diversity and cell type variation. We developed Shiba, robust method integrating assembly, event identification, read counting, statistical analysis, to efficiently quantify exon levels across various types of RNA-seq datasets. Compared existing pipelines, Shiba excels in capturing both annotated unannotated or cryptic differential events with superior accuracy, sensitivity,...
Abstract Genome-wide association studies (GWAS) have been performed to identify thousands of variants in the human genome as disease risk markers, but functional that actually affect gene regulation and their genomic features remain largely unknown. Here we a comprehensive survey regulatory elements genome. We integrated hematopoietic transcription factor (TF) footprints datasets generated by ENCODE project with multiple quantitative trait locus (QTL) (eQTL, caQTL, bQTL, hQTL) investigated...
Abstract Genomic variations are associated with gene expression levels, which called quantitative trait loci (eQTL). Most eQTL may affect the total levels by regulating transcriptional activities of a specific promoter. However, direct exploration genomic promoter using RNA-seq data has been challenging because analyses treat estimated summing those all isoforms transcribed from distinct promoters. Here we propose computational framework for identifying activities, usage (puQTL),...