A5087118776- Cell death mechanisms and regulation
- NF-κB Signaling Pathways
- interferon and immune responses
- Influenza Virus Research Studies
- Immune Response and Inflammation
- Ubiquitin and proteasome pathways
- PARP inhibition in cancer therapy
- Respiratory viral infections research
- Toxin Mechanisms and Immunotoxins
- Viral gastroenteritis research and epidemiology
- Enzyme Structure and Function
- Insect and Pesticide Research
- Microbial Metabolism and Applications
- Protein Kinase Regulation and GTPase Signaling
- Mitochondrial Function and Pathology
- Bacillus and Francisella bacterial research
- Carbohydrate Chemistry and Synthesis
- Advanced optical system design
- Systemic Lupus Erythematosus Research
- Animal Disease Management and Epidemiology
- Glycosylation and Glycoproteins Research
- Advanced Optical Imaging Technologies
- Cell Adhesion Molecules Research
- vaccines and immunoinformatics approaches
- Fibroblast Growth Factor Research
Genomics Research Center, Academia Sinica
2014-2024
National Center for Advancing Translational Sciences
2024
City of Hope
2024
Academia Sinica
2000-2023
National Cheng Kung University
2023
National Taiwan University Hospital
1996-2019
National Taiwan University
1994-2019
Applied Materials (United States)
2018
National Tsing Hua University
2010-2014
Czech Academy of Sciences, Institute of Biotechnology
2013
Abstract Fas-associated protein with death domain (FADD), procaspase-8, and cellular FLICE-inhibitory proteins (cFLIP) assemble through death-effector domains (DEDs), directing receptor signaling towards cell survival or apoptosis. Understanding their three-dimensional regulatory mechanism has been limited by the absence of atomic coordinates for ternary DED complex. By employing X-ray crystallography cryogenic electron microscopy (cryo-EM), we present human FADD-procaspase-8-cFLIP...
Transforming growth factor beta-activated kinase 1 (TAK1), a member of the MAPKKK family, was initially described to play an essential role in transforming beta-signaling pathway, but recent evidence has emerged implicating TAK1 interleukin (IL)-1 and tumor necrosis (TNF) pathways. Notably, two homologous proteins, TAB2 TAB3, have been identified as adaptors linking upstream TRAFs. However, it remains unclear whether interaction between TAB2/TAB3 is necessary for its activation subsequent...
Background The highly pathogenic avian influenza (HPAI) H5N1 virus continues to cause disease in poultry and humans. hemagglutinin (HA) envelope protein is the primary target for subunit vaccine development. Methodology/Principal Findings We used baculovirus-insect cell expression obtain trimeric recombinant HA (rHA) proteins from two HPAI viruses. investigated rHA immunogenicity mice via immunizations, found that highest levels of neutralizing antibodies resulted coupling with a PELC/CpG...
Highly pathogenic avian influenza (HPAI) H5N1 viruses and their transmission capability from birds to humans have raised global concerns about a potential human pandemic. The inherent nature of antigenic changes in has not been sufficiently taken into account immunogen designs for broadly protective HPAI vaccines.We designed hyperglycosylated HA vaccine using N-linked glycan masking on highly variable sequences the HA1 globular head. Immunization these DNA vaccines followed by...
The highly pathogenic avian influenza (HPAI) H5N1 virus, a known trigger of diseases in poultry and humans, is perceived as serious threat to public health. There clear need for broadly protective vaccine or vaccines inducing neutralizing antibodies against multiple clades/subclades. We constructed single, double, triple mutants glycan-masked hemagglutiinin (HA) antigens at residues 83, 127 138 (i.e., g83, g127, g138, g83+g127, g127+g138, g83+g138 g83+g127+g138), then obtained their...
The presence of preferred orientations in single particle analysis (SPA) by cryo-Electron Microscopy (cryoEM) is currently one the hurdles preventing many structural analyses from yielding high-resolution structures. Although existence mostly related to grid preparation, this technical note, we show that some image processing algorithms used for angular assignment and three-dimensional (3D) reconstruction are more robust than others these detrimental conditions. We exemplify argument with...
Highly pathogenic avian influenza H5N1 viruses can result in poultry and occasionally human mortality. A safe effective vaccine is urgently needed to reduce the pandemic potential. Hemagglutinin (HA), a major envelope protein accounting for approximately 80% of spikes virus, often used as antigen subunit development. In this study, we conducted systematic study immune response against virus infection following immunization with recombinant HA proteins expressed insect (Sf9) cells, cells that...
Polyubiquitin chains are regulatory signals for a wide array of biological processes. Recent structural studies reveal novel modes polyubiquitin chain recognition and implicate the diverse repertoire interactions in providing specificity recognition.
ABSTRACT Since dendritic cells may play a key role in defense against influenza virus infection, we examined the effects of recombinant hemagglutinin (HA) proteins derived from mouse-adapted H1N1 (A/WSN/1933), swine-origin 2009 pandemic (A/Texas/05/2009), and highly pathogenic avian H5N1 (A/Thailand/KAN-1/2004) viruses on mouse myeloid (mDCs). The results reveal that tumor necrosis factor alpha (TNF-α), interleukin-12 (IL-12) p70, major histocompatibility complex class II (MHC-II) expression...