A5073657213- CAR-T cell therapy research
- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Acute Myeloid Leukemia Research
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Chronic Lymphocytic Leukemia Research
- Acute Lymphoblastic Leukemia research
- CNS Lymphoma Diagnosis and Treatment
- Mesenchymal stem cell research
- Cancer Immunotherapy and Biomarkers
- Cytomegalovirus and herpesvirus research
- Neutropenia and Cancer Infections
- Lymphoma Diagnosis and Treatment
- Viral Infectious Diseases and Gene Expression in Insects
- Chronic Myeloid Leukemia Treatments
- SARS-CoV-2 and COVID-19 Research
- Polyomavirus and related diseases
- Biosimilars and Bioanalytical Methods
- Clinical Nutrition and Gastroenterology
- Pneumocystis jirovecii pneumonia detection and treatment
- Immunodeficiency and Autoimmune Disorders
- Multiple Myeloma Research and Treatments
- Tuberculosis Research and Epidemiology
Anjo Kosei Hospital
2016-2025
Japanese Red Cross Nagoya Daiichi Hospital
2024
Nagoya University
1988-2021
Tosei General Hospital
2017-2019
The University of Tokyo
2012
T cells genetically modified with a CD19 chimeric antigen receptor (CD19CAR) are remarkably effective against B-cell malignancies in clinical trials. However, major concerns remain regarding toxicities, such as hypogammaglobulinemia, due to aplasia or severe cytokine release syndrome after overactivation of CAR cells. To resolve these adverse events, we aimed develop an inducible system by using tetracycline regulation that would be activated only the presence doxycycline (Dox). In this...
][7][8][9][10][11][12] In particular, patients with B-cell malignancies who have been treated anti-CD20 antibody therapeutics or Bruton's tyrosine kinase inhibitors present much lower anti-SARS-CoV-2 levels after vaccination, and the rate of acquisition correlates inversely interval between discontinuation drugs vaccination. 8,11,12However, there is still limited information about conditions under which such may acquire antibodies vaccination.In this study, we conducted a prospective...
Adoptively transferred CD19 chimeric antigen receptor (CAR) T cells have led to impressive clinical outcomes in B cell malignancies. Beyond induction of remission, the persistence CAR-T is required prevent relapse and provide long-term disease control. To improve function persistence, we developed a composite co-stimulatory domain signaling moiety, CD79A/CD40, induce nuclear translocating signal, NF-κB, synergize with other signals function. CD79A/CD40 incorporating CD19CAR-T (CD19.79a.40z)...
The outcomes of 7/8 allele-matched unrelated bone marrow transplantation (7/8 UBMT) and umbilical cord blood (UCBT) have been improving. We retrospectively analyzed adults with acute leukemia who underwent their first UBMT or UCBT in Japan. Between January 2008 December 2017, a total 4150 patients were recorded, including 488 3662 UCBT. Only 32 had graft-versus-host-disease (GVHD) high-risk HLA mismatched pairs. Overall survival at 3 years was 54% for the group 46% group, nonsignificant...
Electron-radiation-enhanced glide of 30°-Si(g) partial dislocations bringing about an expansion/shrinkage Shockley-type stacking faults in 4H-SiC was observed in-situ by transmission electron microscopy. Geometrical kinks on partials did not migrate the dark, indicating that kink migration is enhanced irradiation. The direction reversible depending irradiation intensity, which can be interpreted terms a sign reversal driving force originating effective fault energy variable with intensity.
Owing to the difficulty in isolating T cells from human biopsy samples, characteristics of that are infiltratinghuman acute graft-versus-host disease (GVHD) tissues remain largely uninvestigated. In present study, TCR-β deep sequencing various GVHD tissue samples and concurrent peripheral blood obtained transplant recipients was performed combination with functional assays tissue-infiltrating cell clones. The repertoire more skewed than blood. frequent clonotypes differed same patient,...
The clinical efficacy of T-cell therapies based on T cells transduced with genes encoding tumor-specific receptors (TCR-T) is related to the in vivo persistence cells. To improve without modifying TCR affinity, we instead modified intracellular signaling, using artificial cell-activating adapter molecules (ATAM), generated by inserting domain (ICD) activating signaling moieties into CD3ζ. ATAMs ICD either CD28 or 4-1BB were generated, assembled complex as a part CD3ζ, and enhanced downstream...
Objective Conditioning regimens for hematopoietic stem cell transplantation (HSCT) are well known to cause severe gastrointestinal toxicities that often disturb the oral intake of patients followed by poor nutrition and life-threatening infection. An elemental diet (ED) is an easily consumed assimilated form liquid nutrients mainly composed amino acids. It alleviates digestive loading from intestine used enteral nutritional support in with Crohn's disease. We herein report, first time,...
Programmed death-ligand 1 (PD-L1) binds to programmed death-1 (PD-1) on activated T cells and contributes T-cell exhaustion. PD-L1 expressed antigen-presenting (APCs) could be thought inhibit the induction of Ag-specific cytotoxic lymphocytes (CTLs) by transducing negative signal into cells; however, roles APCs have not yet been well examined. Therefore, we evaluated in CTLs. CD3 + isolated from cytomegalovirus (CMV)-seropositive healthy donors were stimulated with mature dendritic pulsed...
All-trans retinoic acid (ATRA) and arsenic trioxide (ATO) are essential for acute promyelocytic leukemia (APL) treatment. It has been reported that mutations in PML-RARA confer resistance to ATRA ATO, associated with poor prognosis. Although most were point mutations, we identified a novel seven amino deletion mutation (p.K227_T233del) the RARA region of refractory APL patient. Here, analyzed evolution mutated clone demonstrated (RA). Mutation analysis was performed using samples from...
An artificial T cell adaptor molecule (ATAM) was generated to improve persistence of receptor (TCR) gene-transduced (TCR-T) cells compared such in a preceding study. ATAMs are gene-modified CD3ζ with the intracellular domain 4-1BB inserted middle CD3ζ. NY-ESO-1 TCR-T transduced an ATAM two separated virus vectors demonstrated superior proliferation upon antigen stimulation. To further develop clinically applicable ATAM-transduced cells, we attempted make single vector transduce TCR and...
Almost comparable transplantation outcomes have been reported with HLA-matched unrelated donor (UDT) and cord blood (CBT). We conducted a prospective phase 2 study to assess the efficacy safety of single-unit myeloablative CBT in adult leukemia myelodysplastic syndrome. Because day 180 survival UDT was approximately 80%, we determined alternative hypothesis expected successful engraftment rate 80% set null threshold at 65%. Sixty-two patients (median age, 37 years) were registered, including...