A5003219737- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Tryptophan and brain disorders
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Neuroinflammation and Neurodegeneration Mechanisms
- Systemic Lupus Erythematosus Research
- Monoclonal and Polyclonal Antibodies Research
- Biosensors and Analytical Detection
- Cytokine Signaling Pathways and Interactions
- Extracellular vesicles in disease
- T-cell and Retrovirus Studies
- Multiple Sclerosis Research Studies
- CAR-T cell therapy research
- Cancer Cells and Metastasis
- Advanced Biosensing Techniques and Applications
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Rheumatoid Arthritis Research and Therapies
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Inflammatory Biomarkers in Disease Prognosis
- Sleep and related disorders
- Adipokines, Inflammation, and Metabolic Diseases
Stanford University
2016-2020
Stanford Medicine
2017
Nodality (United States)
2013-2014
Buck Institute for Research on Aging
2011
Cellular aging is linked to deficiencies in efficient repair of DNA double strand breaks and authentic genome maintenance at the chromatin level. Aging poses a significant threat adult stem cell function by triggering persistent damage ultimately cellular senescence. Senescence often considered be an irreversible process. Moreover, critical genomic regions engaged accumulation are unknown. Here we report that 65% naturally occurring repairable self-renewing cells occurs within transposable...
Background: Depression and suicide occur as a comorbidity in chronic medical illness. The poor knowledge attitude of staff nurses general practice greatly impacts the identification treatment these patients. Training on this will reduce mortality related to this. current study identified effectiveness structured educational program improving nursing personnel. Method: A true experimental was performed 120 working different units hospital Saudi Arabia. samples were randomly selected allotted...
Each immunoglobulin isotype has unique immune effector functions. The contribution of these functions in the elimination pathogens and tumors can be determined by monitoring quantitative temporal changes levels. Here, we developed a novel technique using magneto-nanosensors based on effect giant magnetoresistance (GMR) for longitudinal total antigen-specific levels with high precision, as little 1 nL serum. Combining vitro serologic measurements vivo imaging techniques, investigated role...
Abstract Background While technological advances have made it possible to profile the immune system at high resolution, translating high-throughput data into knowledge of mechanisms has been challenged by complexity interactions underlying processes. Tools explore network are critical for better understanding multi-layered processes that underlie function and dysfunction, but require a standardized map interactions. To facilitate this we developed ImmunoGlobe, manually curated intercellular...
Single-cell network profiling (SCNP) is a multiparametric flow cytometry-based approach that simultaneously measures evoked signaling in multiple cell subsets. Previously, using the SCNP approach, age-associated immune responses were identified cohort of 60 healthy donors. In current study, high-dimensional analysis intracellular was performed by measuring 24 nodes 7 distinct subsets within PBMCs an independent 174 donors [144 elderly (>65 yrs); 30 young (25–40 yrs)]. Associations between...
Introduction Alcohol and sedative substance use disorders are escalating global public health challenges. Lebanon has grappled with multiple crises, including economic, healthcare, social issues. Objectives This study aimed to assess the correlates of alcohol risk scores sociodemographic clinical factors, sleep disorders, chronotype, anxiety, depression. Methods A cross-sectional was conducted among Lebanese population using several validated scales use, Alcohol, Smoking, Substance...
Abstract Biomarkers of immune function from viable cells would be high value for many clinical applications. The system responds rapidly to a variety stimuli, so identifying measures that are stable, robust, and relevant features/outcomes can challenging. SCNP is multiparametric flow cytometry assay measuring induced changes in intracellular signaling, providing functional measure pathway capacity multiple cell subsets without physical separation. To assess the longitudinal stability...
<h3>Background</h3> Biomarkers predictive of drug efficacy are lacking in rheumatoid arthritis (RA) and would be useful clinical practice trials. Single cell network profiling (SCNP) is a multiparametric flow cytometry-based assay that measures induced changes (phosphorylation) intracellular signaling proteins, providing functional measure pathway activity immune networking multiple subsets without physical separation. <h3>Objectives</h3> Induced was measured specific monocytes, B T cells...
Abstract Background: SCNP is a multiparametric flow cytometry-based assay that quantitatively and simultaneously measures, at the single cell level, both extracellular surface markers activation levels of intracellular signaling proteins in response to modulation. has been successfully applied hematologic malignancies prognostic predictive analyses. Previous work using bladder washes from cancer patients (pts) established feasibility applying functional characterization epithelial modulation...