A5067193002- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Liver Disease Diagnosis and Treatment
- Microbial Natural Products and Biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Cardiovascular Disease and Adiposity
- Cardiovascular Function and Risk Factors
- Microbial Metabolic Engineering and Bioproduction
- Diabetes Management and Research
- Enzyme Production and Characterization
- Ubiquitin and proteasome pathways
- Pharmacogenetics and Drug Metabolism
- Microbial bioremediation and biosurfactants
- 14-3-3 protein interactions
- Diabetes and associated disorders
- Liver Disease and Transplantation
- Intestinal and Peritoneal Adhesions
- Polyamine Metabolism and Applications
- Enzyme Catalysis and Immobilization
- Advanced Proteomics Techniques and Applications
- Fatty Acid Research and Health
- Ferroptosis and cancer prognosis
- S100 Proteins and Annexins
- Marine Sponges and Natural Products
- Intestinal Malrotation and Obstruction Disorders
University of Duisburg-Essen
2018-2024
Deutsches Diabetes-Zentrum e.V.
2022-2024
Heinrich Heine University Düsseldorf
2022-2024
German Center for Diabetes Research
2022-2023
Disturbed hepatic energy metabolism contributes to non-alcoholic fatty liver (NAFLD), but the development of changes over time and obesity- or diabetes-related mechanisms remained unclear.Two-day old male C57BL/6j mice received streptozotocin (STZ) placebo (PLC) then high-fat (HFD) regular chow diet (RCD) from week 4 (W4) either W8 W16, yielding control [CTRL = PLC + RCD], diabetes [DIAB STZ obesity [OBES HFD] steatohepatitis [NASH models. Mitochondrial respiration was measured by...
Statins are among the most commonly prescribed drugs, and around every fourth person above age of 40 is on statin medication. Therefore, it utmost clinical importance to understand effect statins cancer cell plasticity its consequences not only patients with but also who statins. Here, we find that induce a partial epithelial-to-mesenchymal transition (EMT) phenotype in cells solid tumors. Using comprehensive STRING network analysis transcriptome, proteome, phosphoproteome data combined...
Polycyclic aromatic hydrocarbons (PAHs) are widely distributed pollutants. As oxygen is rapidly depleted in water-saturated PAH-contaminated sites, anaerobic microorganisms crucial for their consumption. Here, we report the metabolic pathway degradation of phenanthrene by a sulfate-reducing enrichment culture (TRIP) obtained from natural asphalt lake. The dominant organism this belongs to Desulfobacteraceae family Deltaproteobacteria and genome-resolved metagenomics led reconstruction its...
Abstract Activity-based protein profiling (ABPP) has emerged as a versatile biochemical method for studying enzyme activity under various physiological conditions, with applications so far mainly in biomedicine. Here, we show the potential of ABPP discovery biocatalysts from thermophilic and lignocellulose-degrading white rot fungus Phanerochaete chrysosporium . By employing comparative ABPP-based functional screen, including direct wood substrate-bound enzymes, identify those carbohydrate...
Plant phytohormone pathways are regulated by an intricate network of signaling components and modulators, many which still remain unknown. Here, we report a forward chemical genetics approach for the identification functional SA agonists in Arabidopsis thaliana that revealed Neratinib (Ner), covalent pan-HER kinase inhibitor drug humans, as modulator signaling. Instead protein kinase, chemoproteomics unveiled Ner covalently modifies surface-exposed cysteine residue epoxide hydrolase isoform...
Intramyocellular lipid content (IMCL) is elevated in insulin-resistant humans, but it changes over time, and relationships with comorbidities remain unclear. We examined IMCL during the initial course of diabetes its associations complications. Participants German Diabetes Study (GDS) recent-onset type 1 (n = 132) or 2 139) glucose-tolerant control subjects 128) underwent 1H-MRS to measure muscle volume, whole-body insulin sensitivity (hyperinsulinemic-euglycemic clamps; M-value), cycling...
Abstract Natural products (NPs) are an important inspirational source for developing drugs and chemical probes. In 1999, the group of Ōmura reported constitutional elucidation zelkovamycin. Although largely unrecognized so far, this NP displays structural similarities as well differences to argyrin family, a class peptidic NPs with promising anticancer activities diverse mode‐of‐action at molecular level. By combination structure experiments, first total synthesis zelkovamycin bioassays,...
Abstract Bioactive natural products are important starting points for developing chemical tools biological research. For elucidating their bioactivity profile, systems with concise complexity such as cell culture frequently used, whereas unbiased investigations in more complex multicellular only rarely explored. Here, we demonstrate the product Rotihibin A and plant research model system Arabidopsis thaliana that transcriptional profiling enables a rapid, label‐free, compound economic...
Background Water T 1 of the liver has been shown to be promising in discriminating progressive forms fatty diseases, inflammation, and fibrosis, yet proper correction for iron lipid is required. Purpose To examine feasibility an empirical approach when measuring imaging‐based validate this by spectroscopy on vivo data. Study Type Retrospective. Population Next mixed lipid‐iron phantoms, individuals with different hepatic content were investigated, including people type diabetes ( N = 15,...
Fragestellung: Insulinresistenz ist ein zentrales pathophysiologisches Merkmal des Typ-2-Diabetes (T2D) und zeigt sich in unterschiedlichem Ausmaß auch bei Typ-1-Diabetes (T1D) Prädiabetes. Ziel dieser Studie war es, Muster der gewebespezifischen zu erkennen, um ihren Beitrag zur metabolischen Heterogenität verstehen.
Estimates of glucose concentrations vary among types blood samples, which impact on the assessment diabetes prevalence. Guidelines recommend a conversion factor to calculate plasma from measurements in whole blood. The American Diabetes Association recommends use drawing tubes containing sodium fluoride (NaF) and citrate, have not yet been evaluated regarding possible differences concentration factors. Thus, we compared NaF-citrate venous estimated prediabetes
This work focuses on an empirical approach to determine water T1 from multiparametric MR images, including T1, PDFF and T2* maps. To this end, a multiple linear regression model was fit describe the deviation in MOLLI based values, which were measured phantoms built at increasing lipids iron content. method validated cohort of healthy volunteers diabetes subjects (n=45). Further investigations conducted elucidate relationship between before after correction for hepatic lipid content, liver...
Continuous Glucose Monitoring Following Consumption of Everyday Foods and their Effect on Postprandial Insulin Levels
<p>Intramyocellular lipid content (IMCL) is elevated in insulin-resistant humans, but its changes over time and relationships with comorbidities remain unclear. We examined IMCL during the initial course of diabetes associations complications. Participants German Diabetes Study (GDS) recent-onset type 1 (n=132) or 2 (n=139) glucose-tolerant controls (n=128) underwent 1H-magnetic resonance spectroscopy to measure muscle volume, whole-body insulin sensitivity (hyperinsulinemic-euglycemic...
<p>Intramyocellular lipid content (IMCL) is elevated in insulin-resistant humans, but its changes over time and relationships with comorbidities remain unclear. We examined IMCL during the initial course of diabetes associations complications. Participants German Diabetes Study (GDS) recent-onset type 1 (n=132) or 2 (n=139) glucose-tolerant controls (n=128) underwent 1H-magnetic resonance spectroscopy to measure muscle volume, whole-body insulin sensitivity (hyperinsulinemic-euglycemic...
Abstract Proteolysis is a key catalytic event in protein and thus cellular homeostasis. Despite the importance wide implications of proteolytic processing degradation, methods describing degradation folded proteins at high temporal spatial resolution are not well established. However, this information required to obtain deep mechanistic understanding events their consequences. Here, we describe an integrated method comprising time-resolved mass spectrometry, circular dichroism spectroscopy...