A5007003023- SARS-CoV-2 and COVID-19 Research
- HIV/AIDS drug development and treatment
- T-cell and Retrovirus Studies
- HIV Research and Treatment
- SARS-CoV-2 detection and testing
- Animal Disease Management and Epidemiology
- Mosquito-borne diseases and control
- Viral Infections and Outbreaks Research
- HIV/AIDS Research and Interventions
- Biosensors and Analytical Detection
- Circadian rhythm and melatonin
- Free Radicals and Antioxidants
- Metabolism, Diabetes, and Cancer
- Synthesis of Indole Derivatives
- Cervical Cancer and HPV Research
- Liver Disease Diagnosis and Treatment
- Biochemical and Molecular Research
- Pancreatic function and diabetes
- GABA and Rice Research
- Chromosomal and Genetic Variations
- Viral-associated cancers and disorders
- Nanotechnology research and applications
- Cancer Genomics and Diagnostics
- Hepatitis C virus research
- Animal Virus Infections Studies
Kumamoto University
2022-2025
Matsumoto University
2021-2022
BRAC University
2017-2019
Virus-related cancers are malignancies caused by specific viruses, such as human papillomavirus (HPV), hepatitis B virus, and T-cell leukemia contributing significantly to the global cancer burden through persistent infection oncogenic transformation. The current study aimed develop a robust HPV-16 detection method for formalin-fixed specimens. To prevent false negatives resulting from DNA fragmentation, quality check step was added. Additionally, this used multiplex polymerase chain...
Determinants of HIV-1 latency establishment are yet to be elucidated. HIV reservoir comprises a rare fraction infected cells that can survive host and virus-mediated killing. In vitro reporter models so far offered feasible means inspect this population, but with limited capabilities dissect provirus silencing dynamics. Here, we describe new model, HIV-Timer cell kinetics activity (HIV-Tocky) dual fluorescence spontaneous shifting reveal reactivation This unique feature allows, for the first...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has acquired multiple mutations since its emergence. Analyses of the SARS-CoV-2 genomes from infected patients exhibit a bias toward C-to-U mutations, which are suggested to be caused by apolipoprotein B mRNA editing enzyme polypeptide-like 3 (APOBEC3, A3) cytosine deaminase proteins. However, role A3 enzymes in replication remains unclear. To address this question, we investigated effect family proteins on THP-1 cells lacking A3A...
Cancer has several pathways by which it is developed in our body. Among them folic acid biosynthetic pathway one where dihydrofolate reductase (DHFR) enzyme converts into tetrahydrofolate leads to unwanted and uncontrollable growth of tissues. Our aim this study design DHFR antagonistic potential small molecules that inhibits Folic Acid Biosynthetic Pathways. In study, Human obtained from Protein Data Bank (PDB) docked with established anticancer drugs including Afatinib, Doxorubicin,...
In the search of safer and more effective drugs while reducing costs increasing productivity novel drug discovery, scientists are changing their focus to an approach known as repurposing. This involves finding a new therapeutic effect already existing drug. It is method that can effectively be addressed in discovery development challenges targeting different disorders. Many which have failed clinical trials for not being intended indication also been repurposed has great benefit...
Although BLV has been eradicated in some European countries, is still endemic other including Japan and the United States. EBL causes huge economic damage to cattle industry.
Abstract Retroviruses integrate viral genome DNA into the host cellular genome. The integrated proviral serves as a template for production of progeny viruses. There are many points similarity between two human retroviruses, T-cell leukemia virus type 1 (HTLV-1) and immunodeficiency (HIV-1), such size, structure targeting cells; however, their natural course in infected individuals is remarkably different. HTLV-1 induces latent infection even absence anti-retroviral drugs but enhances...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has acquired multiple mutations since its emergence. Analyses of the SARS-CoV-2 genomes from infected patients exhibit a bias toward C-to-U mutations, which are suggested to be caused by apolipoprotein B mRNA editing enzyme polypeptide-like 3 (APOBEC3, A3) cytosine deaminase proteins. However, role A3 enzymes in replication remains unclear. To address this question, we investigated effect family proteins on myeloid leukemia cell...
Highly transmissible viruses including SARS-CoV-2 frequently accumulate novel mutations that are detected via high-throughput sequencing. However, there is a need to develop an alternative rapid and non-expensive approach. Here we developed multiplex DNA detection method Intelli-OVI for analysing existing of SARS-CoV-2.
Background: The incidence of non-B non-C hepatocellular carcinoma (NBNC-HCC), which is negative for hepatitis B surface antigen and C virus antibodies, on the rise. Relatively high numbers NBNC-HCC patients are core antibody (HBcAb) positive, suggesting that previous HBV infection may play a role in development, though exact mechanisms unclear. This study aimed to investigate whether genomes integrated into host genome HBcAb-positive cases how these integrations contribute cancer development...
Psychiatric diseases relentlessly distress the ability of work also productivity a person's life. Considering phenotypic unpredictability amongst patients, it is very hard to understand pathogenesis. The study aims identify pathways responsible for psychiatric disease, especially depression. Initially 62 depression associated proteins were listed from UniProt and then hub genes those identified. For functional annotation analysis, IDs submitted Database Annotation, Visualization Integrated...
Eradication of HIV-1 latently infected cells is an important issue in HIV treatment. However, there are limited models available to assess therapeutic efficacy vitro. Here, we present a protocol for establishing variety HIV-infected Jurkat cells, including productive and latent status, evaluating the antiviral agents, followed by PCR/sequencing-based detection replication competent provirus. This useful optimization treatment provides insights into mechanisms clonal selection heterogeneous...
Abstract The stably integrated pool of HIV-1 proviruses in the host genome stands against curative strategies. This reservoir is extremely heterogeneous with respect to cell type, anatomical location, integration site, and replication fitness. During initial phase infection, only a few infected cells can resist immune clearance or cytopathic effect establish this resistant pool. mechanisms underlying HIV latency initiation are not fully resolved yet. In current study, we propose validate new...
<title>Abstract</title> High transmissible viruses including SARS-CoV-2 frequently accumulate novel mutations that are detected via high-throughput sequencing. However, there is a need to develop an alternative rapid and non-expensive approach. Here we developed multiplex DNA detection method Intelli-OVI for analysing existing of SARS-CoV-2. uses the micro-disc-based IntelliPlex computational algorithms objective variant identification (OVI). micro-discs printed with unique pictorial pattern...
Abstract Determinants of HIV-1 latency establishment are yet to be elucidated. HIV reservoir comprises a rare fraction infected cells that can survive host and virus-mediated killing. In vitro reporter models so far offered feasible means inspect this population, but with limited capabilities dissect provirus silencing dynamics. Here, we describe new model; HIV-Timer cell kinetics activity (HIV-Tocky) dual fluorescence spontaneous shifting reveal reactivation This unique feature allowed; for...
ABSTRACT Bovine leukemia virus (BLV), a retrovirus, causes Enzootic Leukosis (EBL) in cattle following latent infection period. The BLV results polyclonal expansion of infected B-lymphocytes and ∼5% develop monoclonal leukosis. Since the clonal virus-infected cell is key pathogenesis EBL, assessing clonality malignant cells crucial for both understanding viral pathogenesis, which might be useful EBL diagnosis. For investigation BLV-infected non-EBL cattle, two methods were used to evaluate...
ABSTRACT A recent pandemic of SARS-CoV-2 infection has caused severe health problems and substantially restricted social economic activities. To cope with such an outbreak, the identification infected individuals high accuracy is vital. qRT-PCR plays a key role in diagnosis infection. The N protein-coding region widely analyzed for Japan. We recently encountered two cases SARS-CoV-2-positive specimens showing atypical amplification curves qRT-PCR. performed whole-genome sequencing found that...