A5026260197- Cardiac Valve Diseases and Treatments
- Aortic aneurysm repair treatments
- Aortic Disease and Treatment Approaches
- Nitric Oxide and Endothelin Effects
- Cardiac Structural Anomalies and Repair
- Autophagy in Disease and Therapy
- Sirtuins and Resveratrol in Medicine
- Peroxisome Proliferator-Activated Receptors
- Cardiovascular Issues in Pregnancy
- Infective Endocarditis Diagnosis and Management
- Infectious Aortic and Vascular Conditions
- Cardiac electrophysiology and arrhythmias
- Cardiac and Coronary Surgery Techniques
- Hippo pathway signaling and YAP/TAZ
- Adipose Tissue and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Cardiac Arrhythmias and Treatments
- Cardiovascular Function and Risk Factors
- Cholesterol and Lipid Metabolism
- Mitochondrial Function and Pathology
- Cardiac Imaging and Diagnostics
- Cardiac, Anesthesia and Surgical Outcomes
- Atrial Fibrillation Management and Outcomes
- Mechanical Circulatory Support Devices
- Vascular anomalies and interventions
National Yang Ming Chiao Tung University
2015-2025
Ministry of Health and Welfare
2003-2025
Taipei Veterans General Hospital
2015-2025
Tao Yuan General Hospital
2023
National Taipei University
2010-2021
National Yang Ming University Hospital
2007-2020
Cardiovascular Research Center
2018
Ministry of Health and Welfare
2018
Rutgers New Jersey Medical School
2008-2016
Istituto Neurologico Mediterraneo
2016
Silent information regulator 1 (Sirt1), a class III histone deacetylase, retards aging and protects the heart from oxidative stress. We here examined whether Sirt1 is protective against myocardial ischemia/reperfusion (I/R).Protein mRNA expression of significantly reduced by I/R. Cardiac-specific Sirt1(-/-) mice exhibited significant increase (44±5% versus 15±5%; P=0.01) in size infarction/area at risk. In transgenic with cardiac-specific overexpression Sirt1, both risk (15±4% 36±8%;...
Background— Mitochondrial autophagy is an important mediator of mitochondrial quality control in cardiomyocytes. The occurrence and its significance during cardiac hypertrophy are not well understood. Methods Results— Mice were subjected to transverse aortic constriction (TAC) observed at multiple time points up 30 days. Cardiac developed after 5 days, the ejection fraction was reduced 14 heart failure days TAC. General upregulated between 1 12 hours TAC but downregulated below physiological...
NAD+ acts not only as a cofactor for cellular respiration but also substrate NAD(+)-dependent enzymes, such Sirt1. The synthesis is regulated by both the de novo and salvage pathways. Nicotinamide phosphoribosyltransferase (Nampt) rate-limiting enzyme in pathway.Here we investigated role of Nampt mediating cardiac myocytes function heart vivo.Expression was significantly decreased ischemia, ischemia/reperfusion pressure overload. Upregulation increased ATP concentrations, whereas...
Energy stress, such as ischemia, induces mitochondrial damage and death in the heart. Degradation of damaged mitochondria by mitophagy is essential for maintenance healthy survival. Here, we show that during myocardial ischemia was mediated predominantly through autophagy characterized Rab9-associated autophagosomes, rather than well-characterized form dependent on autophagy-related 7 (Atg) conjugation system LC3. This played an role protecting heart against a protein complex consisting...
Rationale: NF2 (neurofibromin 2) is an established tumor suppressor that promotes apoptosis and inhibits growth in a variety of cell types, yet its function cardiomyocytes remains largely unknown. Objective: We sought to determine the role cardiomyocyte ischemia/reperfusion (I/R) injury heart. Methods Results: investigated isolated mouse myocardium at baseline response oxidative stress. was activated subjected H 2 O murine hearts I/R. Increased expression promoted activation Mst1 (mammalian...
Rationale: The Hippo pathway plays an important role in determining organ size through regulation of cell proliferation and apoptosis. inactivation consequent activation YAP (Yes-associated protein), a transcription cofactor, have been proposed as strategy to promote myocardial regeneration after infarction. However, the long-term effects deficiency on cardiac function under stress remain unknown. Objective: We investigated effect presence pressure overload (PO). Methods Results: used mice...
In congestive heart failure the balance between cell death and survival in cardiomyocytes is compromised. Sirtuin 1 (Sirt1) activates machinery has been shown to be protective against ischemia/reperfusion injury murine heart. The role of Sirt1 failure, especially human hearts not clear.The expression other (associated) downstream molecules from patients with advanced was examined. down-regulated (54.92% ± 7.80% samples compared healthy control cardiomyocytes). modulation involved...
Obesity induces cardiomyopathy characterized by hypertrophy and diastolic dysfunction. Whereas mitophagy mediated through an Atg7 (autophagy related 7)-dependent mechanism serves as essential to maintain mitochondrial quality during the initial development of obesity cardiomyopathy, Rab9 (Ras-related protein Rab-9A)-dependent alternative takes over role chronic phase. Although it has been postulated that DRP1 (dynamin-related 1)-mediated fission consequent separation damaged portions...
NADPH oxidases (Noxes) produce ROS that regulate cell growth and death. NOX4 expression in cardiomyocytes (CMs) plays an important role cardiac remodeling injury, but the posttranslational mechanisms modulate this enzyme are poorly understood. Here, we determined FYN, a Src family tyrosine kinase, interacts with C-terminal domain of NOX4. FYN colocalized perinuclear mitochondria, ER, nuclear fractions CMs, negatively regulated NOX4-induced O2– production apoptosis CMs. Mechanistically, found...
Background and objectives Elevated plasma level of asymmetric dimethylarginine (ADMA) have been reported to be associated with endothelial dysfunction atherosclerosis risk factors, may predict cardiovascular events in patients ESRD. In this study, we aimed assess the association between ADMA long-term outcome a cohort stage 3 4 chronic kidney disease (CKD). Design, setting, participants, & measurements From July 2006 June 2009, 298 consecutive CKD scheduled undergo coronary angiography were...
Background— Heart failure is accompanied by changes in cardiac metabolism characterized reduced fatty acid (FA) utilization. However, the underlying mechanism and its causative involvement progression of heart are poorly understood. The peroxisome proliferator activated receptor-α (PPARα)/retinoid X receptor (RXR) heterodimer promotes transcription genes involved FA through binding to PPAR response element, called direct repeat 1 (DR1). Silent information regulator (Sirt1) a histone...
Background Uncomplicated type B aortic dissections was regarded benign and treated with optimal medical therapy (OMT). However, studies showed highly unpredictable of disease progression, which suggested the need earlier intervention. To search for features associated worse outcomes OMT is important due to risk We investigated mortality remodeling between aggressive (OMT pre-emptive endovascular intervention) conservative necessary operations). Methods Retrospective analysis performed in...