A5052071276- COVID-19 Clinical Research Studies
- Sepsis Diagnosis and Treatment
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Electrochemical Analysis and Applications
- Electrochemical sensors and biosensors
- Metabolomics and Mass Spectrometry Studies
- Computational Drug Discovery Methods
- Advanced Proteomics Techniques and Applications
- Kruppel-like factors research
- Caveolin-1 and cellular processes
- Reproductive System and Pregnancy
- Angiogenesis and VEGF in Cancer
- Peroxisome Proliferator-Activated Receptors
- Pregnancy and preeclampsia studies
- Respiratory viral infections research
- Sphingolipid Metabolism and Signaling
- Glutathione Transferases and Polymorphisms
- Metabolism and Genetic Disorders
- Lipid metabolism and disorders
- Advanced biosensing and bioanalysis techniques
- Genomics, phytochemicals, and oxidative stress
- Eicosanoids and Hypertension Pharmacology
- Wnt/β-catenin signaling in development and cancer
- Fungal and yeast genetics research
Cardiff University
2018-2024
Universidade Nova de Lisboa
2013-2021
Rede de Química e Tecnologia
2013-2021
Institute of Infection and Immunity
2021
Centre for Immunity, Infection and Evolution
2020
INCLIVA Health Research Institute
2012-2020
Mechanistic studies have established a biological role of sterol metabolism in infection and immunity with clinical data linking deranged cholesterol during sepsis poorer outcomes. In this systematic review we assess the relationship between biomarkers homeostasis mortality critical illness.
Abstract Oxylipins are potent biological mediators requiring strict control, but how they removed en masse during infection and inflammation is unknown. Here we show that lipopolysaccharide (LPS) dynamically enhances oxylipin removal via mitochondrial β-oxidation. Specifically, genetic or pharmacological targeting of carnitine palmitoyl transferase 1 (CPT1), a importer fatty acids, reveal many oxylipins by this protein in vitro vivo. Using stable isotope-tracing lipidomics, find...
The lipid envelope of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an essential component the virus; however, its molecular composition undetermined. Addressing this knowledge gap could support design antiviral agents as well further our understanding viral-host protein interactions, infectivity, pathogenicity, and innate immune system clearance. Lipidomics revealed that virus comprised mainly phospholipids (PLs), with some cholesterol sphingolipids,...
Abstract Summary We present LipidFinder online, hosted on the LIPID MAPS website, as a liquid chromatography/mass spectrometry (LC/MS) workflow comprising peak filtering, MS searching and statistical analysis components, highly customized for interrogating lipidomic data. The online interface of includes several innovations such comprehensive parameter tuning, search engine employing in-house customized, curated computationally generated databases multiple reporting/display options. A set...
Alterations in the redox balance are involved origin, promotion and progression of cancer. Inter-individual differences oxidative stress regulation can explain a part variability cancer susceptibility. The aim this study was to evaluate if polymorphisms genes codifying for different systems levels have role susceptibility breast We analyzed 76 single base located 27 by SNPlex technology. First, we tested all selected SNPs 493 patients 683 controls replicated significant results second...
Abdominal aortic aneurysm (AAA) is an inflammatory vascular disease with high mortality and limited treatment options. How blood lipids regulate AAA development unknown. Here lipidomics genetic models demonstrate a central role for procoagulant enzymatically oxidized phospholipids (eoxPL) in regulating AAA. Specifically, through activating coagulation, eoxPL either promoted or inhibited depending on tissue localization. Ang II administration to ApoE-/- mice increased intravascular...
Abstract The current pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) poses a global health crisis and will likely continue to impact public for years. As the effectiveness innate immune response is crucial patient outcome, huge efforts have been made understand how dysregulated responses may contribute progression. Here we reviewed knowledge cellular SARS-CoV-2 infection, highlighting areas further investigation suggesting potential...
Background: Common chromosome 9p21 single nucleotide polymorphisms (SNPs) increase coronary heart disease risk, independent of traditional lipid risk factors. However, lipids comprise large numbers structurally related molecules not measured in measurements, and many have inflammatory bioactivities. Here, we applied lipidomic genomic approaches to 3 model systems characterize metabolic changes common Chr9p21 SNPs, which confer ≈30% elevated associated with altered expression ANRIL, a long...
Abstract The coronavirus infectious disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome 2 (SARS-CoV-2) remains a world health concern and can cause high mortality in susceptible groups. While vaccines offer chance to treat disease, prophylactic anti-viral treatments are still of vital importance, especially context the mutative ability this group viruses. Therefore, it is essential elucidate molecular mechanisms viral entry, innate sensing immune evasion SARS-CoV-2,...
We present LipidFinder 2.0, incorporating four new modules that apply artefact filters, remove lipid and contaminant stacks, in-source fragments salt clusters, a isotope deletion method which is significantly more sensitive than available open-access alternatives. also incorporate novel false discovery rate method, utilizing target-decoy strategy, allows users to assess data quality. A renewed profiling introduced searches three different databases from LIPID MAPS returns bulk structures...
Diverse polymorphisms have been associated with the predisposition to develop cancer. On fewer occasions, they related evolution of disease and different responses treatment. Previous studies our group on genes oxidative stress (rs3736729 GCLC rs207454 XDH) DNA damage repair (rs1052133 OGG1) a breast In present work, we evaluated hypothesis that these also play role in patient’s survival. A population-based cohort study 470 women diagnosed primary cancer median follow up 52.44 months was...
Introduction Diagnosing neonatal sepsis is heavily dependent on clinical phenotyping as culture-positive body fluid has poor sensitivity, and existing blood biomarkers have specificity. A combination of machine learning, statistical deep pathway biology analyses led to the identification a tripartite panel biologically connected immune metabolic markers that showed greater than 99% accuracy for detecting bacterial infection with 100% sensitivity. The cohort study described here designed...
Angiogenesis process contributes to the pathogenesis of B-cell chronic lymphocytic leukemia (B-CLL) being levels VEGFA and bFGF higher in patients than healthy controls. Our aim was evaluate implication angiogenesis factors genetic variants predisposition B-CLL their association with clinical survival. We performed a population-based case-control study 224 Spanish 476 randomly selected controls susceptibility developing B-CLL. Six polymorphisms were evaluated: rs1109324, rs1547651, rs3025039...