A5069692819- Influenza Virus Research Studies
- Respiratory viral infections research
- RNA and protein synthesis mechanisms
- Immune Response and Inflammation
- Animal Disease Management and Epidemiology
- Neonatal Respiratory Health Research
- HIV Research and Treatment
- Plant Virus Research Studies
- Viral Infections and Immunology Research
- vaccines and immunoinformatics approaches
- interferon and immune responses
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- RNA Research and Splicing
- Animal Virus Infections Studies
- RNA regulation and disease
- RNA modifications and cancer
- Congenital Diaphragmatic Hernia Studies
- Neuroscience of respiration and sleep
- Escherichia coli research studies
- Virus-based gene therapy research
- Extracellular vesicles in disease
- Monoclonal and Polyclonal Antibodies Research
- Infection Control and Ventilation
- Viral Infections and Outbreaks Research
Chulabhorn Hospital
2017-2024
Mahidol University
2005-2017
Siriraj Hospital
2005-2017
Harvard University
2005
Avian influenza viruses preferentially recognize sialosugar chains terminating in sialic acid-alpha2,3-galactose (SAalpha2,3Gal), whereas human SAalpha2,6Gal. A conversion to SAalpha2,6Gal specificity is believed be one of the changes required for introduction new hemagglutinin (HA) subtypes population, which can lead pandemics. H5N1 virus a major threat emergence pandemic virus. As 12 June 2007, has been reported 45 countries, and 312 cases with 190 deaths have confirmed. We describe here...
Highly pathogenic H5N1 avian influenza virus has spread through at least 45 countries in three continents. Despite the ability to infect and cause severe disease humans, cannot transmit efficiently from human human. The lack of efficient transmission indicates incompletion adaptation new host species. required mutations for complete emergence a potential pandemic are likely originate be selected within infected tissues. Differential receptor preference plays an important role species-tropism...
The codon usage pattern is a specific characteristic of each species; however, the all genes in genome not uniform. Intriguingly, most viruses have patterns that are vastly different from optimal their hosts. How viral with efficiently expressed during infection unclear. An analysis similarity between and individual host has never been performed. In this study, we demonstrated human RNA similar to some genes, especially those involved cell cycle. This finding was substantiated by its...
Abstract Microarray analysis of gene expression profile lungs from two fatal H5N1 influenza cases identified 3,435 genes with higher than twofold changes in mRNA levels as compared to those normal lung. One thousand nineteen and 2,416 were up‐regulated down‐regulated commonly, respectively. Gene ontology several terms significant association these genes, most which are related cellular metabolism regulation process including apoptosis chemotaxis. Pulmonary surfactant protein D (SP‐D) was...
Niclosamide has been known to inhibit a number of pH-dependent viruses via the neutralization endosomal acidic pH. It also shown disrupt mTORC1 signaling pathway. The replication many requires activation. Here, we investigated inhibitory activity niclosamide against HIV-1, and determined whether inhibition was involved. cytotoxicity anti-HIV-1 were tested in TZM-bl SupT1 cells. showed dose- time-dependent HIV-1 replication, but did not involve reverse transcription steps. mechanism explored...
Schlafen (SLFN) proteins are a subset of interferon-stimulated early response genes with antiviral properties. An mechanism SLFN11 was previously demonstrated in human immunodeficiency virus type 1 (HIV-1)-infected cells, and it shown that inhibited HIV-1 production codon usage-specific manner. The usage patterns many viruses vastly different from those their hosts. inhibition expression by suggests may be able to inhibit other suboptimal pattern. However, the effect on replication influenza...
Abstract The highly pathogenic avian influenza A (H5N1) virus is a virulent that causes an acute febrile respiratory disease with high mortality in humans. To gain better insight of H5N1 viral distributions infected human tissues, the levels RNA were determined autopsy tissues from two patients who by using real‐time reverse transcription‐polymerase chain reaction. In one patient died on day 6 illness, load lung was extremely high, whereas other organs more than log lower. 17 similar and...
ABSTRACT The effectiveness of neutralizing monoclonal antibodies against emerging variants with spike protein mutations has been limited. Early high-titer convalescent plasma therapy (CPT) tends to be effective and a potential treatment option for COVID-19 outpatient treatment. An open-label, 1:1, randomized controlled trial compared the efficacy high titer CPT favipiravir (CPT-FPV) versus sotrovimab. study aimed evaluate non-inferiority CPT-FPV sotrovimab in preventing hospitalization...
Piwpankaew Y, Monteerarat Suptawiwat O, Puthavathana P, Uipresertkul M, Auewarakul P. Distribution of viral RNA, sialic acid receptor, and pathology in H5N1 avian influenza patients. APMIS 2010; 118: 895–902. Avian virus infects causes severe disease humans. Despite the increasing number fatal cases 15 countries threat pandemic, detailed pathologic virologic findings humans are limited. In this study, we describe histopathologic findings, distributions RNA Neu5Acα2‐3Galβ1‐4 receptor human...
Background Influenza viruses bind and infect respiratory epithelial cells through sialic acid on cell surface. Differential preference to types contributes host- tissue-tropism of avian seasonal influenza viruses. Although the highly pathogenic virus H5N1 can cause severe diseases in humans, it is not efficient infecting human upper tract. This because scarcity its receptor, α2,3-linked acid, airway. Expression be influenced by various factors including inflammatory process. Allergic...
Respiratory secretions, such as saliva and bronchoalveolar fluid, contain anti-influenza activity. Multiple soluble factors have been described that exert activity are believed to be responsible for the in respiratory secretions. It was previously shown a bronchial epithelial cell culture could produce exosome-like particles with Whether extracellular vesicles secretions is unknown. Therefore, we characterized lavage fluid found microparticles, which mostly stained positive markers both...
In this study, the effect of innate serum inhibitors on influenza virus infection was addressed. Seasonal A(H1N1) and A(H3N2), 2009 pandemic (H1N1pdm) highly pathogenic avian (HPAI) A(H5N1) viruses were tested with guinea pig sera negative for antibodies against all these as evaluated by hemagglutination-inhibition microneutralization assays. presence inhibitors, each inhibited differently measured amount viral nucleoprotein produced in Madin-Darby canine kidney cells. The seasonal A(H3N2)...
Microparticles (MPs) are vesicles that released by budding from plasma membrane of living cells. Recently, the role MPs in antiviral activity has been proposed. We investigated quantity and anti-influenza human alveolar epithelial cells A549, bronchial BEAS-2B, colon adenocarcinoma HT-29, lung fibroblast MRC-5. were found all four cell lines. However, against an H1N1 influenza virus was only A549 BEAS-2B. BEAS-2B differentiation did not increase MP release. Methyl-β-cyclodextrin (MβCD)...
Oral cavity can be an entry site of influenza virus and saliva is known to contain innate soluble anti‐influenza factors. Influenza strains were shown vary in their susceptibility those antiviral Whether the factors plays any role host species specificity viruses not known. In this study, activity human chicken against H5N1 avian investigated by hemagglutination inhibition (HI) neutralization (NT) assays. comparison viruses, isolates showed reduced as measured HI NT Interestingly, isolate...