To dissect the antibiotic role of nanostructures from chemical moieties belligerent to both bacterial and mammalian cells, here we show antimicrobial activity cytotoxicity nanoparticle-pinched polymer brushes (NPPBs) consisting chemically inert silica nanospheres systematically varied diameters covalently grafted with hydrophilic that are non-toxic non-bactericidal. Assembly polymers into nanostructured NPPBs doesn't alter their amicability but it incurs a transformation potential against...

Electrostatics in aqueous media is commonly understood terms of screened Coulomb interactions, where like-charged objects, such as polyelectrolytes, always repel. These intuitive expectations are based on mean field theories, the Poisson–Boltzmann formalism, which routinely used colloid science and computational biology [Israelachvili, J. (1992) Intermolecular Surface Forces (Academic, London), 2nd ed.]. Like-charge attractions, however, have been observed a variety systems [Gelbart, W. M.,...

High-surface-area mesoporous bioactive glass materials have been developed, which demonstrate accelerated deposition rates of hydroxyapatite when immersed in simulated body fluids. Also, a novel blood-clotting application for materials, composed microspheres (see TEM image), has designed. Both bone growth and blood clotting are surface dependent hence the effect changing shape particles on vitro rate hemostatic efficacy investigated.

MicroRNA155 plays an important role in many solid malignancies. Expression and function of miR-155 laryngeal carcinoma have not been fully understood. This study aims to investigate the expression squamous cell (LSCC), relationship between its downstream target suppressor cytokine signaling 1 (SOCS1)-STAT3 pathway, related clinicopathological factors. Sixty-three samples twenty-one control mucosa obtained from total laryngectomy cases were analyzed using Western blot analysis real-time PCR....

HA-coated CNTs are very stable in aqueous solution and able to transport through porous media a significant extent, indicating high environmental mobility. Use of magnetic metaloxide nanoparticles as affinity probes provides permanent efficient removal strategy for from (see image).

We report here a green chemistry method to conjugate hydrophobic payloads (Lilial as prototype) highly efficiently (35.8 wt %) with (1→4)-2-amino-2-deoxy-β-d-glucan (i.e., chitosan) via Schiff base bond formation in an ionic liquid, which renders chitosan easily dissolvable common organic solvents and amenable further functional modifications. As example, thermoresponsive poly(N-isopropylacrylamide) was grafted the chitosan−Lilial conjugate. The graft copolymer self-assembled water at...

Abstract Styrene-maleic acid copolymers allow for solubilization and reconstitution of membrane proteins into nanodiscs. These polymer-encased nanodiscs are promising platforms studies in a near-physiologic environment without the use detergents. However, current styrene-maleic display severe limitations terms buffer compatibility ensued flexibility various applications. Here, we present new family that do not aggregate at low pH or presence polyvalent cations, can be used to solubilize...

This study evaluated the effects of air humidity on spontaneous combustion characteristics coal. Four levels relative (dry air, 30%, 60%, and 100%) were used as experimental flow. The crossing point temperature (CPT) oxidation products measured using a self-designed system. We found CPT values 153°C, 151°C, 150°C, 148°C for four humidity, respectively. concentration production increased with an increase in humidity. Then, thermal coal studied thermogravimetric differential scanning...

Self-assembled DNA delivery systems based on anionic lipids (ALs) complexed with mediated by divalent cations have been recently introduced as an alternative to cationic lipid–DNA complexes because of their low cytotoxicity. We investigate AL–DNA induced different using synchrotron small angle x-ray scattering and confocal microscopy show how ion-mediated interactions are expressed in the self-assembled structures phase behavior complexes. The governing complex: ions can mediate strong...

<i>Objective:</i> To investigate the expression of special AT-rich sequence-binding protein 1 (SATB1), and relationship between SATB1 clinicopathological factors laryngeal squamous cell carcinoma (LSCC). <i>Methods:</i> Real-time PCR, Western blot immunohistochemistry were used to analyze 80 samples LSCC 25 control mucosa. The parameters was evaluated. <i>Results:</i> mRNA levels in 2.5- 7.5-fold higher than those mucosa tissues (p < 0.001). detected...

Abstract Membrane proteins can be reconstituted in polymer-encased nanodiscs for studies under near-physiological conditions and the absence of detergents, but traditional styrene-maleic acid copolymers used this purpose suffer severely from buffer incompatibilities. We have recently introduced zwitterionic amide (zSMAs) to overcome limitation. Here, we compared extraction reconstitution membrane into lipid by a series zSMAs with different styrene:maleic molar ratios, chain sizes, molecular...

A wide range of biomineralization and templating methods exist for organizing inorganic materials at a length-scales. Here, we show that crystallographic control the nanostructures is possible using synthetic biomolecular templates comprised anionic DNA cationic membranes, which self-assemble into multilamellar structure where periodic one-dimensional (1D) lattice parallel chains confined between stacked two-dimensional (2D) lipid sheets. We have organized Cd2+ ions within interhelical pores...

A 'bricks-and-mortar' assembly approach can be used to create well- defined fibers on the microscale with alternating organic-inorganic arrangement through a simple ice-templating strategy. The are constructed from closely packed monodisperse inorganic nanoparticles@PNIPAm core/shell microgels. series of functional 'bricks', such as and hollow nanoparticles prepared.

Proteorhodopsin is the membrane protein used by marine bacterioplankton as a light-driven proton pump. Here, we describe rapid cooperative assembly process directed universal electrostatic interactions that spontaneously organizes proteorhodopsin molecules into ordered arrays with well defined orientation and packing density. We demonstrate charge density-matching mechanism selectively controls process. The among different components in system are tuned varying their densities to yield...

A "brick-and-mortar" assembly approach for creating porous carbon and carbon/metal oxide fibers on the micron scale with well-defined pore structure interface is presented. series of monodisperse silica@polyacrylonitrile (PAN) silica@metal oxide@PAN core/shell particles were synthesized by emulsion polymerization assembled into organic–inorganic composite through a simple ice-templating strategy assistance polyvinyl alcohol. Porous well-controlled pores interfaces created oxidative...

Cellular membranes are natural nanoengineering devices, where matter transport, information processing, and energy conversion across the nanoscale boundaries mediated by membrane proteins (MPs). Despite great potential of MPs for nanotechnologies, their broad utility in engineered systems is limited fluidic often labile nature MP-supporting membranes. Little known on how to direct spontaneous reconstitution into robust synthetic nanomembranes or tune MP functions through rational design...

Manipulating recognition and transport at the nanoscale holds great promise for technological breakthroughs in energy conversion, catalysis, information processing. Living systems evolve specialized membrane proteins (MPs) embedded lipid bilayers to exquisitely control communications across insulating boundaries. Harnessing MP functions directly synthetic opens up enormous opportunities nanotechnology, but there exist fundamental challenges of how address labile nature that renders them...

The prevalent wisdom on developing membrane active antimicrobials (MAAs) is to seek a delicate, yet unquantified, cationic–hydrophobic balance. Inspired by phages that use nanostructured protein devices invade bacteria efficiently and selectively, we study here the antibiotic role of nanostructures designing spherical rod-like polymer molecular brushes (PMBs) mimic two basic structural motifs bacteriophages. Three model PMBs with different well-defined geometries consisting multiple,...

Amphipathic styrene-maleic acid (SMA) copolymers directly solubilize biomembranes into SMA-lipid particles, or SMALPs, that are often regarded as nanodiscs and hailed a native membrane platform. The promising outlook of SMALPs inspires the discovery many SMA-like also putative nanodiscs, but fundamental question remains on how much SMALP analogues truly resemble bilayer structure nanodiscs. This unfortunate ambiguity undermines utility SMA in biology because function proteins depend...