A5090772403- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Enzyme Structure and Function
- Plant biochemistry and biosynthesis
- Plant tissue culture and regeneration
- Microbial Metabolic Engineering and Bioproduction
- Protein Structure and Dynamics
- Plant Gene Expression Analysis
- Legume Nitrogen Fixing Symbiosis
- Plant Molecular Biology Research
- Phytase and its Applications
- Genomics and Phylogenetic Studies
- Protein purification and stability
- Transgenic Plants and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- ATP Synthase and ATPases Research
- Bacterial Genetics and Biotechnology
- Protist diversity and phylogeny
- Weed Control and Herbicide Applications
- Animal Genetics and Reproduction
- Nitrogen and Sulfur Effects on Brassica
- Connective tissue disorders research
- Ubiquitin and proteasome pathways
- Phytoestrogen effects and research
- Retinoids in leukemia and cellular processes
Yunnan Normal University
2011-2024
Kunming Medical University
2022
East China Normal University
2007-2008
Ludwig-Maximilians-Universität München
1999-2003
Summary 5′ and 3′ untranslated regions (UTRs) of plastid RNAs act as regulatory elements for post‐transcriptional control gene expression. Polyethylene glycol‐mediated transformation with UTR–GUS reporter fusions was used to study the function psb A, rbc L rpl 32 UTRs in vivo. All were expressed from same promoter, i.e. promoter 16S‐rRNA gene, such that variations RNA protein levels would be due involved UTR alone. Transgenic tobacco lines containing different combinations showed fivefold...
BackgroundJatropha curcas L., also called the Physic nut, is an oil-rich shrub with multiple uses, including biodiesel production, and currently exploited as a renewable energy resource in many countries. Nevertheless, because of its origin from tropical MidAmerican zone, J. confers inherent but undesirable characteristic (low cold resistance) that may seriously restrict large-scale popularization. This adaptive flaw can be genetically improved by elucidating mechanisms underlying plant...
Summary Sequences described as chloroplast DNA replication origins were analysed in vivo by creating deletion and insertion mutants via plastid transformation tobacco. Deletion of the oriA sequence, which is located within intron trnI gene, resulted heteroplastomic transformants, when selection marker was inserted intron. Removal complete sequence together with however, yielded homoplastomic transformants normal phenotype, wild‐type signals no longer detectable through Southern analysis,...
Methamphetamine (MA) is spread worldwide and a highly addictive psychostimulant that can induce neurodegeneration cognitive disorder, which lacks effective treatments. We other researchers have found the crucial member of Hsp70 chaperone machinery, DnaJ, liable to be co-aggregated with aberrant proteins, has been confirmed risk factor promote neurodegeneration. In current study, we demonstrated tailing hyper-acidic fusion partner, tua2, human DnaJB1 could resist formation toxic mutant Tau...
Phenylalanine ammonia-lyase (PAL) is the key entry enzyme of plant phenylpropanoid pathway. It plays an important role in biosynthesis podophyllotoxin, anti-tumor lignan that currently produced from its main natural source Sinopodophyllum hexandrum (Royle) Ying. In this study, we cloned gene ShPAL encoding phenylalanine by RT-PCR root S. ecotype inhabited Aba' district, Sichuan, based on public SRA transcriptome data-package. Bioinformatics analyses showed ShPAL-encoded protein composed 711...
C-reactive protein (CRP) is widely used as a biomarker of inflammation. It plays important roles in innate immunity response member pattern recognition receptors, by binding oxidation-specific epitopes including some intermediates lipid oxidative chain reaction. The inferred antioxidative ability CRP was ever demonstrated only few vitro evidences, and needs to be clarified especially vivo. Herein, we expressed human three representative non-animal organisms (Escherichia coli, Saccharomyces...
Nowadays, available phosphorus (P) deficiency in soil and weed resistance to herbicides have emerged as two severe limiting factors for sustainable agriculture. Therefore, it is of urgent needs improve plant absorption/utilization ability the P, seek phosphate (Pi)-alternative P fertilizers, develop new forms control systems. Phosphite (Phi), a resource relatively high amount only less than Pi Earth, can be converted utilizable uniquely some bacterial species by oxidization via its specific...
Catalase (CAT) plays a crucial role in plant responses to environmental stresses and maintaining redox homeostasis. However, its putative heat lability might compromise activity function, thus restricting thermotolerance. Herein, we verified Arabidopsis CAT3 was of poor thermostability that then engineered by fusion expression Escherichia coli. We found our selected partners, three hyperacidic mini-peptides the short rubredoxin from hyperthermophile Pyrococcus furiosus, were commonly...
Membrane-bound pyrophosphatases (PPases) are involved in the adaption of organisms to stress conditions, which was substantiated by numerous plant transgenic studies with H+-PPase yet devoid any correlated evidences for other two subfamilies, Na+-PPase and Na+,H+-PPase. Herein, we demonstrate gene cloning functional evaluation membrane-bound PPase (CmPP) human gut microbe Clostridium methylpentosum. The CmPP encodes a single polypeptide 699 amino acids that predicted as multi-spanning...