A5020772854- Mosquito-borne diseases and control
- HIV Research and Treatment
- Trypanosoma species research and implications
- Autophagy in Disease and Therapy
- SARS-CoV-2 and COVID-19 Research
- Traditional and Medicinal Uses of Annonaceae
- Studies on Chitinases and Chitosanases
- SARS-CoV-2 detection and testing
- Piperaceae Chemical and Biological Studies
- Neurobiology and Insect Physiology Research
- Insect Resistance and Genetics
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Insect and Pesticide Research
- Poxvirus research and outbreaks
- Endoplasmic Reticulum Stress and Disease
- Physiological and biochemical adaptations
- Reproductive Biology and Fertility
- Alkaloids: synthesis and pharmacology
- Transgenic Plants and Applications
- Biosensors and Analytical Detection
- Insect symbiosis and bacterial influences
- Medicinal Plants and Neuroprotection
- Virus-based gene therapy research
- Insect and Arachnid Ecology and Behavior
Universidade Federal do Rio de Janeiro
2017-2024
Fundação Oswaldo Cruz
2018-2023
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive stranded RNA virus which has caused the recent deadly pandemic called COVID-19. The SARS-CoV-2 virion coated with a heavily glycosylated Spike glycoprotein responsible for attachment and entry into target cells. One, as yet unexploited strategy preventing infections, targeting of glycans on Spike. Lectins are carbohydrate-binding proteins produced by plants, algae, cyanobacteria. Some lectins can neutralize...
Community testing is a crucial tool for the early identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and transmission control. The emergence highly mutated Omicron variant (B.1.1.529) raised concerns about its primary site replication, impacting sample collection detectability by rapid antigen tests. We tested performance Panbio diagnostic test (Ag-RDT) using nasal oral specimens COVID-19 diagnosis in 192 symptomatic individuals, with quantitative...
In oviparous animals, the egg yolk is synthesized by mother in a major metabolic challenge, where different components are secreted to hemolymph and delivered oocytes mostly endocytosis. The macromolecules then stored wide range of endocytic-originated vesicles which collectively referred as organelles occupy most mature cytoplasm. After fertilization, contents these degraded regulated manner supply embryo cells with fundamental molecules for de novo synthesis. Yolk accumulation its...
Rhodnius prolixus is a hematophagous insect, vector of Chagas disease. After feeding, as blood slowly digested, amino acids are used substrates to fuel lipid synthesis, and adult females accumulate lipids in the fat body produce eggs. In order evaluate importance de novo fatty acid synthesis for this insect metabolism, we generated acetyl-CoA carboxylase (ACC) deficient insects. The knockdown (AccKD) had delayed digestion shorter lifespan. Their bodies showed reduced lipogenesis activity,...
Among the most malignant cancers, oral squamous cell carcinoma (OSCC) stands out as common head and neck tumor. Despite advances in field of treatment, prognosis patients with OSCC remains poor. Aiming to overcome limitations currently existing therapies against OSCC, present work aims investigate potential photodynamic therapy (PDT) phenothiazine derivatives used alone or combination. The incorporation methylene blue (MB) toluidine (TB) was evaluated lines (HSC-3 SCC-9) a nontumor line...
Follicular atresia is the mechanism by which oocyte contents are degraded during oogenesis in response to stress conditions, allowing energetic resources stored developing oocytes be reallocated optimize female fitness. Autophagy a conserved intracellular degradation pathway where double-membrane vesicles formed around target organelles leading their after lysosome fusion. The autophagy-related protein 8 (ATG8) conjugated autophagic membrane and has key role elongation closure of...
Most vectors of arthropod-borne diseases produce large eggs with hard and opaque eggshells. In several species, it is still not possible to induce molecular perturbations the embryo by delivery molecules using microinjections or eggshell permeabilization without losing viability, which impairs basic studies regarding development population control. Here we tested properties permeability R. prolixus, a Chagas disease vector, aim deliver pharmacological inhibitors egg cytoplasm allow...
In insects, synthesis and deposition of the chorion (eggshell) are performed by professional secretory follicle cells (FCs) that surround oocytes in course oogenesis. Here, we found ULK1/ATG1, an autophagy-related protein, is highly expressed FCs Chagas-Disease vector Rhodnius prolixus, parental RNAi silencing ULK1/ATG1 results with abnormal ultrastructure presenting expanded rough ER membranes as well increased expression chaperone BiP3, both indicatives stress. Silencing LC3/ATG8, another...
In nearly every species of insect, embryonic development takes place outside the mother's body and is entirely dependent on elements that mother had previously stored within eggs. It well known follicle cells (FCs) synthesize eggshell (chorion) components during process choriogenesis, final step oogenesis before fertilization. These have developed a specialization in massive production chorion proteins, which are essential for protection survival embryo. Here, we investigate function Sec16,...
The phytochemical investigation of the alkaloid-rich fraction obtained from leaves Guatteria pogonopus Mart.(Annonaceae) allowed isolation and identification for first time in this species of: (+)-nornuciferine (1), a mixture 1 (+)-anonaine (2), (+)-isocorydine (3), (+)-nuciferine (4), (+)-roemerine ( 5), (-)-tetrahydropseudocolumbamine 6), 6, liriodenine (9) lysicamine (10), 1,2,9-trimethoxy-10-hydroxyaporphine (7) bulbocapnine (8), 9, 10, (+)-N-methyllindicarpine (11).Compounds 7, 8, 11...
The phytochemical investigation of the hexane and methanol extracts from bark Duguetia pycnastera Sandwith (Annonaceae) afforded seven known compounds, two asarone-derived phenylpropanoids five isoquinoline-derived alkaloids.The asarones, γ-asarone (1-allyl-2,4,5-trimethoxybenzene) 2,4,5-trimethoxy-styrene were isolated extract while aporphine alkaloids, O-methylmoschatoline, lysicamine, nornuciferidine, guatterine N-oxide, benzyltetrahydroisoquinoline alkaloid, (S)-reticuline alkaloid...
BACKGROUND In 2022, an outbreak of mpox that started in European countries spread worldwide through human-to-human transmission. Cases have been mostly mild, but severe clinical presentations reported. these cases, tecovirimat has the drug choice to treat patients with aggravated disease. OBJECTIVES Here we investigated susceptibility 18 isolates monkeypox virus (MPXV) obtained from different regions Brazil. METHODS Different concentrations were added cell monolayers infected each MPXV...
Abstract Background Despite antiretroviral treatment efficacy, it does not lead to the complete eradication of HIV infection. Consequently, reactivation virus from latently infected cell reservoirs is a major challenge toward cure efforts. Two strategies targeting viral latency are currently under investigation: “shock and kill” “block lock.” The “Block Lock” methodology aims control HIV-1 reactivation, promoting functional cure. We utilized CRISPR/dCas9-KRAB platform, which was initially...
Autophagy and the ubiquitin-proteasome system (UPS) are important cellular mechanisms that coordinate protein degradation essential for proteostasis. P62/SQSTM1 is a receptor cargo able to deliver ubiquitinated targets proteasome proteolytic complex and/or autophagosome. In insect vector of Chagas disease, Rhodnius prolixus , previous works have shown knockdown different autophagy-related genes (ATGs) ubiquitin-conjugating enzymes resulted in abnormal oogenesis phenotypes embryo lethality....
Abstract Background: Despite antiretroviral treatment efficacy, it does not lead to the complete eradication of HIV infection. In addition, HIV-1 latency reactivation is a major challenge towards cure efforts. Two strategies infection, “shock and kill” based on from patient, “block lock". The “Block Lock” methodology aims control reactivation, promoting functional cure. KRAB fused CRISPR/dCas9 (pdCas9KRAB) system was previously produced cell transcription. Based this construct we developed...
Abstract Community testing is a crucial tool for the early identification of SARS-CoV-2 infection and transmission control. The emergence highly mutated Omicron variant (B.1.1.259) raised concerns about its primary site replication, impacting sample collection, detectability by rapid antigens tests. We tested Antigen Rapid Diagnostic Test (Ag-RDT) performance using nasal, oral, saliva specimens COVID-19 diagnosis in 192 symptomatic individuals, RT-qPCR from nasopharyngeal samples as Variant...