A5027706362- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Pomegranate: compositions and health benefits
- Metabolism and Genetic Disorders
- ATP Synthase and ATPases Research
- Adipose Tissue and Metabolism
- Muscle Physiology and Disorders
- Long-Term Effects of COVID-19
- Nutrition and Health in Aging
- Neuroscience and Neuropharmacology Research
- Paraoxonase enzyme and polymorphisms
- Antioxidant Activity and Oxidative Stress
- Electron Spin Resonance Studies
- Calcium signaling and nucleotide metabolism
- Sesame and Sesamin Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Ginkgo biloba and Cashew Applications
- Genetics, Aging, and Longevity in Model Organisms
- Genetic Neurodegenerative Diseases
- Cerebral Palsy and Movement Disorders
- Stroke Rehabilitation and Recovery
- Phytochemicals and Antioxidant Activities
- Intensive Care Unit Cognitive Disorders
- Advanced NMR Techniques and Applications
- Skin Protection and Aging
École Polytechnique Fédérale de Lausanne
2023-2025
Amazentis (Switzerland)
2023-2025
Newcastle University
2020-2025
Wellcome Centre for Mitochondrial Research
2020-2025
Université du Québec à Montréal
2017-2023
University of Newcastle Australia
2023
The brain's ability to process complex information relies on the constant supply of energy through aerobic respiration by mitochondria. Neurons contain three anatomically distinct compartments—the soma, dendrites, and projecting axons—which have different energetic biochemical requirements, as well mitochondrial morphologies in cultured systems. In this study, we apply quantitative three-dimensional electron microscopy map network morphology complexity mouse brain. We examine somatic,...
Mitophagy is a quality control mechanism that eliminates damaged mitochondria, yet its significance in mammalian pathophysiology and aging has remained unclear. Here, we report mitophagy contributes to mitochondrial dysfunction skeletal muscle of aged mice human patients. The early disease stage characterized by fibers with central nuclei, enhanced around these nuclei. However, progressive halts disrupts lysosomal homeostasis. Interestingly, activated or halted occur mosaic manner even...
Aging is associated with a progressive decline in muscle mass and strength, process known as sarcopenia. Evidence indicates that mitochondrial dysfunction plays causal role sarcopenia suggests alterations dynamics/morphology may represent an underlying mechanism. Caloric restriction (CR) amongst the most efficient non-pharmacological interventions to attenuate rodents thought exert its beneficial effects by improving function. However, CR on morphology dynamics, especially aging muscle,...
Abstract Mitochondria play a crucial role in maintaining cellular health. It is interesting that the shape of mitochondria can vary depending on type cell, mitochondrial function, and other conditions. However, there are limited studies link functional assessment with morphology evaluation at high magnification, even fewer do so situ none human muscle biopsies. Therefore, we have developed method which combines through Cytochrome c Oxidase (COX) histochemistry, 3D electron microscopy (EM)...
Summary Mitochondrial shape and function are known to be linked; therefore, there is a need combine three‐dimensional EM structural analysis with functional analysis. Cytochrome c oxidase labelling one approach examine mitochondrial at the level. However, previous efforts apply this method have had several issues including inconsistent results, disruption ultrastructure, lack of optimisation for volume methods. We used short fixation microwave processing address these issues. show that our...
Septic patients frequently develop skeletal muscle wasting and weakness, resulting in severe clinical consequences adverse outcomes. Sepsis triggers sustained induction of autophagy, a key cellular degradative pathway, muscles. However, the impact enhanced autophagy on sepsis-induced dysfunction remains unclear. Using an inducible muscle-specific Atg7 knockout mouse model (Atg7iSkM-KO), we investigated functional importance sepsis using cecal ligation puncture model. Atg7iSkM-KO mice...
Abstract Background Mitochondrial disease is a heterogenous group of rare, complex neurometabolic disorders. Despite their individual rarity, collectively mitochondrial diseases represent the most common cause inherited metabolic disorders in UK; they affect 1 every 4300 individuals, up to 15,000 adults (and similar number children) UK. manifests multisystem and isolated organ involvement, commonly affecting those tissues with high energy demands, such as skeletal muscle. Myopathy...
Summary The brain’s ability to process complex informations relies on the constant supply of energy through aerobic respiration by mitochondria. Neurons contain three anatomically distinct compartments – soma, dendrites, and projecting axons which have different energetic biochemical requirements, as well mitochondrial morphologies in cultured systems. Here we apply a quantitative three-dimensional electron microscopy approach map network morphology complexity mouse brain. We examine...
Abstract Cardiovascular diseases remain the primary cause of global mortality, necessitating effective strategies to alleviate their burden. Mitochondrial dysfunction is a driving force behind aging and chronic conditions, including heart disease. Here, we investigate potential Urolithin A (UA), gut microbiome-derived postbiotic that enhances mitophagy, ameliorate both age-related decline in cardiac function failure. We highlight significance targeting mitochondria, by comparing gene...
Abstract Septic patients frequently develop skeletal muscle wasting and weakness, resulting in severe clinical consequences adverse outcomes. Autophagy is a stress-induced degradative process essential to cell survival. Recent studies have demonstrated that sepsis triggers sustained induction of autophagy muscles, although the impact this enhanced on sepsis-induced dysfunction remains unclear. Atg7 an gene plays major role autophagosome formation. Using inducible muscle-specific knockout...
Abstract Urolithin A is a gut microbiome derived postbiotic that has been shown to stimulate mitophagy, and improve muscle mitochondrial health when administered orally humans. In three separate randomized trials, we have now investigated the effect of topical administration on skin aging features UVB-mediated photodamaged skin. Post-menopausal women with evidence such as > Grade 3 wrinkle formation were included in split-face/arm study design first trial ( 1; n=48 ), followed by second...
Abstract Mitochondria play a crucial role in maintaining cellular health. It is interesting that the shape of mitochondria can vary depending on type cell, mitochondrial function, and other conditions. The morphology this network maintained by balance between fusion fission, necessary for proper functioning energetics as well maintenance DNA (mtDNA) (Chan, 2006; Friedman Nunnari, 2014; Glancy et al., 2015). If two processes slightly perturbed, dramatic changes occur. However, there are...
With aging, skeletal muscles progressively loose mass and function, a biological process known as sarcopenia. Recent data indicate that accumulation of mitochondrial dysfunction plays major role in the development sarcopenia also suggested alterations mitophagy dynamics/morphology may represent mechanisms causing this dysfunctional mitochondria aged muscles. One most efficient lifestyle non‐pharmacological intervention to attenuate rodents is calorie restriction (CR). The hypothesis...
Rationale Sepsis‐induced skeletal muscle dysfunction contributes to physical disability, and increased mortality in intensive care unit patients. Autophagy is a catabolic process by which cells degrade their own components. Recent studies indicate that sepsis triggers sustained induction of autophagy muscles; however, the impact on sepsis‐induced contractile metabolic dysfunctions remains unclear. In this study, we evaluated functional importance dysfunction. Methods Selective inhibition...