A5002313205- Antifungal resistance and susceptibility
- Epigenetics and DNA Methylation
- Advanced Breast Cancer Therapies
- Genomics and Chromatin Dynamics
- Plant Disease Resistance and Genetics
- Berry genetics and cultivation research
- Plant Pathogens and Fungal Diseases
- Fungal and yeast genetics research
- DNA Repair Mechanisms
- Fungal Infections and Studies
- PARP inhibition in cancer therapy
- Cancer Treatment and Pharmacology
- Plant tissue culture and regeneration
- Genomics and Phylogenetic Studies
- RNA Research and Splicing
- Breast Cancer Treatment Studies
- Folate and B Vitamins Research
- Plant-Microbe Interactions and Immunity
- HER2/EGFR in Cancer Research
- Microbial Natural Products and Biosynthesis
- Animal Genetics and Reproduction
- Primary Care and Health Outcomes
- Psidium guajava Extracts and Applications
- Cancer, Lipids, and Metabolism
- Gut microbiota and health
National Institute of Agricultural Botany
2022-2025
King's College Hospital
2012-2021
University of Kent
2016-2019
Royal Cornwall Hospital
2016-2019
Breast Cancer Now
2019
Guy's and St Thomas' NHS Foundation Trust
2019
King's College Hospital NHS Foundation Trust
2019
Barts Health NHS Trust
2016-2019
National Health Service
2016
University of Southampton
2014-2016
Nitric oxide is generated from sweat nitrite in the acidic environment of skin surface and thought to contribute protection against infection. This study examined sensitivity Trichophyton mentagrophytes, T. rubrum, Candida albicans, Streptococcus pyogenes, Staphylococcus aureus Propionibacterium acnes acidified nitrite.Organisms were cultured varying concentrations pH for different lengths time, before being transferred recovery medium. With exception Strep. addition increased antimicrobial...
Transcriptionally silent heterochromatin is associated with repetitive DNA. It poorly understood whether and how differs between different organisms its structure can be remodelled in response to environmental signals. Here, we address this question by analysing the chromatin state DNA repeats human fungal pathogen Candida albicans. Our analyses indicate that, contrary model systems, each type of element assembled into a distinct state. Classical Sir2-dependent hypoacetylated hypomethylated...
Cultivated raspberries ( Rubus idaeus L.) most commonly bear small, red, highly aromatic fruits. Their colour is derived predominantly from anthocyanins, water soluble polyphenolic pigments, but as well red forms, there exist cultivars that display yellow- and apricot-coloured In this investigation, we used a multi-omics approach to elucidate the genetic basis of apricot fruit in raspberry. Using metabolomics, quantified anthocyanins raspberry fruits demonstrated that, contrast red-fruited...
Quorn mycoprotein, a protein-rich meat alternative, is produced through large-scale fermentation of the fungus Fusarium venenatum. However, major challenge during F. venenatum consistent appearance mutants called colonial variants (C-variants). These C-variants have highly branched morphology, which ultimately lead to less desirable final product and early termination process. This study aimed identify genetic mutations responsible for C-variant morphology. We first isolated both wild-type...
Eukaryotic genomes are packaged into chromatin structures that play pivotal roles in regulating all DNA-associated processes. Histone posttranslational modifications modulate structure and function, leading to rapid regulation of gene expression genome stability, key steps environmental adaptation. Candida albicans, a prevalent fungal pathogen humans, can rapidly adapt thrive diverse host niches. The contribution C. albicans biology is largely unexplored. Here, we generated the first...
Red raspberry (Rubus idaeus L.) is an economically valuable soft-fruit species with a relatively small (~300 Mb) but highly heterozygous diploid (2n = 2x 14) genome. Chromosome-scale genome sequences are vital tool in unravelling the genetic complexity controlling traits of interest crop plants such as red raspberry, well for functional genomics, evolutionary studies, and pan-genomics diversity studies. In this study, we developed primocane fruiting variety ('Autumn Bliss') floricane...
UHRF1 (ubiquitin-like, containing PHD and RING finger domains 1) has a well-established role in epigenetic regulation through the recognition of various histone marks interaction with chromatin-modifying proteins. However, its function regulating cell cycle progression remains poorly understood been largely attributed to transcriptional regulation. In this study we have used Xenopus laevis egg extracts analyse Uhrf1 DNA replication absence influences. We demonstrate that removal inhibits...
The effect of folic acid (FA) on breast cancer (BC) risk is uncertain. We hypothesised that this uncertainty may be due, in part, to differential effects FA between BC cells with different phenotypes. To test we investigated the treatment concentrations within range unmetabolised reported humans expression transcriptome non-transformed (MCF10A) and cancerous (MCF7 Hs578T) cells. total number transcripts altered was: MCF10A, seventy-five (seventy up-regulated); MCF7, twenty-four (fourteen...
Centromeres, sites of kinetochore assembly, are important for chromosome stability and integrity. Most eukaryotes have regional centromeres epigenetically specified by the presence histone H3 variant CENP-A. CENP-A chromatin is often surrounded pericentromeric regions packaged into transcriptionally silent heterochromatin. Candida albicans, most common human fungal pathogen, possesses small assembled chromatin. The state C. albicans unknown. Here, first time, we address this question. We...
Dietary supplementation with folic acid (FA) has been shown to induce opposing effects on cancer-related outcomes. The mechanism underlying such heterogeneity is unclear. We hypothesized that FA induces changes in breast cancer–associated (BRCA) genes 1 and 2 expression function through altered epigenetic regulation a cell type–dependent manner. investigated the effect of treating normal cancer cells physiologically relevant concentrations mRNA protein expression, capacity for DNA repair,...
Abstract This study presents the first genome and transcriptome analyses for Fusarium oxysporum f.sp. lactucae (Fola) which causes wilt disease of lettuce. Long-read sequencing three race 1 (Fola1) 4 (Fola4) isolates revealed key differences in putative effector complement between races with other F. f.spp. following mimp -based bioinformatic analyses. Notably, homologues Secreted Xylem ( SIX ) genes, also present many f.spp, were identified Fola, both SIX9 SIX14 (multiple copies sequence...
<title>Abstract</title> <italic><bold>Background</bold></italic> Quorn mycoprotein, a protein-rich meat alternative, is produced through large-scale fermentation of the fungus <italic>Fusarium venenatum</italic>. However, major challenge during <italic>F. venenatum </italic>fermentation consistent appearance mutants called colonial variants (C-variants). These C-variants have highly branched morphology, which ultimately lead to less desirable final product and early termination process. This...
A delicate balance between genome stability and instability ensures integrity while generating genetic diversity, a critical step for evolution. Indeed, excessive is harmful, moderated can drive adaptation to novel environments by maximising variation. Candida albicans , human fungal pathogen that colonises different parts of the body, adapts rapidly frequently hostile host microenvironments. In this organism, ability generate large-scale genomic variation key adaptative mechanism triggering...
Abstract Background: Preclinical and clinical data support a key role for the PI3K pathway in resistance to endocrine therapy patients with ER+ breast cancer suggest that combining inhibitors may overcome resistance. Short-term preoperative window of opportunity (WOO) studies are validated strategy novel treatments provide proof-of-concept define most appropriate patient population by directly assessing treatment effects tumour tissue before after treatment. This is first WOO study inhibitor...
Abstract Background: Although preclinical data suggest that combining PI3K inhibitors with endocrine therapy may overcome resistance, results from randomized clinical trials have failed to identify a subgroup of patients derive substantial benefit. This preoperative window study assessed whether adding the inhibitor pictilisib can increase anti-tumor effects anastrozole in primary breast cancer and aimed most appropriate patient population for combination therapy. Methods: In this...
ABSTRACT The human fungal pathogen Candida albicans is responsible for millions of infections annually. Due to the few available anti-fungal drugs and increasing incidence drug resistance, number C. dramatically increasing. Morphological switches, such as white-opaque switch yeast-hyphae switch, are key development pathogenic traits. Lysine deacetylases emerging important regulators morphological switches. Yet, targeting lysine problematic due high homology between proteins that could result...
<title>Abstract</title> Background Plant breeding played a very important role in transforming strawberries from being niche crop with small geographical footprint into an economically grown across the planet. But even modern marker assisted takes considerable amount of time, over multiple plant generations, to produce desirable traits. As quicker alternative, plants traits can be raised through tissue culture by doing precise genetic manipulations. Overexpression morphogenic regulators...
This study presents the first genome and transcriptome analyses for
Plant breeding played a very important role in transforming strawberries from being niche crop with small geographical footprint into an economically grown across the planet. But even modern marker assisted takes considerable amount of time, over multiple plant generations, to produce desirable traits. As quicker alternative, plants traits can be raised through tissue culture by doing precise genetic manipulations. Overexpression morphogenic regulators previously known for meristem...
Abstract Background: The OPPORTUNE Study randomized postmenopausal patients (pts) to receive 2-week preoperative treatment with anastrozole (ANA) plus pictilisib ("ANA+PIC" arm) or ANA alone. Patients had newly diagnosed, operable, ER+, HER2- invasive breast cancer of ≥1 cm size. primary outcome at interim analysis (n=70) revealed that the addition PIC significantly increased anti-proliferative response as measured by reduction in Ki67 immunohistochemistry (IHC). Multivariate analyses...