A5056642994- PI3K/AKT/mTOR signaling in cancer
- Extracellular vesicles in disease
- Reproductive System and Pregnancy
- Eicosanoids and Hypertension Pharmacology
- Pharmacogenetics and Drug Metabolism
- MicroRNA in disease regulation
- Sperm and Testicular Function
- Pregnancy and preeclampsia studies
- Liver physiology and pathology
- Vitamin D Research Studies
- Pancreatic function and diabetes
- Reproductive Biology and Fertility
- Inflammasome and immune disorders
- Wnt/β-catenin signaling in development and cancer
- Cancer, Lipids, and Metabolism
- Antimicrobial Peptides and Activities
- Cancer, Hypoxia, and Metabolism
- Tryptophan and brain disorders
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- Stress Responses and Cortisol
- Dupuytren's Contracture and Treatments
- Genital Health and Disease
- Surgical Sutures and Adhesives
- Cancer Mechanisms and Therapy
University of Tartu
2012-2025
Vähiuuringute Tehnoloogia Arenduskeskus
2015
Extracellular vesicles (EVs), including exosomes and microvesicles (<200 nm), play a vital role in intercellular communication carry net negative surface charge under physiological conditions. Zeta potential (ZP) is popular method to measure the of EVs, while used as an indicator charge, colloidal stability influenced by chemistry, bioconjugation, theoretical model applied. Here, we investigated effects such factors on ZP well-characterized EVs derived from human choriocarcinoma JAr cells....
While the cellular mechanisms of liver regeneration have been thoroughly studied, role extracellular matrix (ECM) in is still poorly understood. We utilized a proteomics-based approach to identify shifts ECM composition after CCl4 or DDC treatment and studied their effect on proliferation cells by combining biophysical cell culture methods. identified notable alterations structural components (eg collagens I, IV, V, fibronectin, elastin) as well non-structural proteins olfactomedin-4,...
Extracellular vesicles (EVs) are membrane-bound biological nanoparticles (NPs) and have gained wide attention as potential biomarkers. We aimed to isolate characterize EVs from media conditioned by individually cultured preimplantation bovine embryos assess their relationship with embryo quality. Presumptive zygotes were in 60 μl droplets of culture media, 50 collected the either on day 2, 5 or 8 post-fertilization. After sampling, cultures continued remaining until 8, development was...
The period of time when the embryo and endometrium undergo significant morphological alterations to facilitate a successful implantation-known as "window implantation"-is critical moment in human reproduction. Embryo communicate extensively during this period, lipid bilayer bound nanoscale extracellular vesicles (EVs) are purported be integral communication.To investigate nature EV-mediated embryo-maternal communication, we have supplemented trophoblast analogue spheroid (JAr) derived EVs an...
The effects of commercial enzymes (pectinases, cellulases, beta-1-3-glucanases, and pectin lyases) on the recovery anthocyanins polyphenols from blackcurrant press cake were studied considering two solid:solvent ratios (1:10 1:4 w/v). β-glucanase enabled highest total phenolic content - 1142 mg/100 g, extraction was similar using all (∼400 g). use cellulases pectinases enhanced antioxidants (DPPH 1080 g; CUPRAC 3697 freeze-dried extracts presented antioxidant potential (CUPRAC, DPPH), which...
Abstract Background Successful establishment of pregnancy hinges on appropriate communication between the embryo and uterus prior to implantation, but nature this remains poorly understood. Here, we tested hypothesis that endometrium is receptive embryo-derived signals in form RNA. Methods We have utilized a non-contact co culture system simulate conditions pre implantation environment uterus. bioorthogonally tagged embryonic RNA tracked transferred transcripts endometrium. Transferred were...
Obesity is an emerging health problem worldwide as it associated with increased risk of cardiovascular, metabolic, mental disorders, and cancer. Therapeutic weight management remains one the options for treatment excess comorbidities. In this study, therapeutic potential elocalcitol, a fluorinated derivative vitamin D, was studied on model high-fat diet (HFD)-induced obesity in mice. It demonstrated that co-administration elocalcitol doses 15 ug/kg (i.p.) twice week 16 weeks prevented body...
Cell-free RNAs have the potential to act as a means of gene expression regulation between cells and are therefore used diagnostic markers describing state tissue environment. The origin functions such in human ovarian follicle, environment oocyte maturation, unclear. current study investigates difference microRNA profiles fertile women polycystic ovary syndrome (PCOS) patients three compartments from same preovulatory follicle: mural granulosa (MGC), cell-free follicular fluid (FF),...
While follicular fluid (FF) is well known to provide an optimal environment for oogenesis, its functional roles following release into the oviduct during ovulation are currently elusive. We hypothesized that FF and FF-derived extracellular vesicles (EVs) may be conveyors of signals capable inducing functionally-relevant transcriptional responses in oviductal cells. The aim this study was, therefore, evaluate effect EVs on transcriptome primary bovine epithelial cells (BOECs). examined gene...
While follicular fluid (FF) is known to enhance the functional properties of spermatozoa, role FF-derived extracellular vesicles (EVs) in this respect unknown. We hypothesized that bovine FF EVs convey signals spermatozoa supporting sperm viability, inducing capacitation and acrosome reaction. In study, effects on functions are evaluated. Irrespective size follicles which had originated from, they were capable These specific source EVs, as human-cell-line-derived or porcine did not affect...
Psoriasis vulgaris (PsV) and psoriatic arthritis (PsA) are inflammatory diseases with unresolved pathophysiological aspects. Extracellular vesicles (EVs) play an important role in intercellular communication. We compared the miRNA contents surface proteome of EVs blood serum PsV PsA patients to healthy controls. Size-exclusion chromatography was used isolate from 12 patients, control subjects. EV samples were characterized RNA sequencing identify differentially enriched EV-bound miRNAs....
Abstract Vitamin D deficiency is a worldwide health concern, especially in the elderly population. Much remains unknown about relationship between vitamin (VDD), stress‐induced cognitive dysfunctions and depressive‐like behaviour. In this study, 4‐month‐old male C57Bl/6J mice were fed with control or free diet for 6 months, followed by unpredictable chronic stress (UCMS) 8 weeks. VDD induced impairment reduced grooming behaviour, but did not induce While UCMS sufficient expected phenotype...
The Wnt/β-catenin pathway regulates key cellular processes such as differentiation, proliferation, apoptosis; and its activation promotes development of several cancer types. Expression CD43 (leukosialin), the predominant leukocyte transmembrane sialoglycoprotein, has been detected in many tumors non-hematopoietic origin. participates cell adhesion intracellular signal transduction pathways involved proliferation survival. cytoplasmic domain reported to translocate nucleus, interact with...
The serine/threonine kinase AKT/PKB has a critical role in the regulation of cell proliferation. Because AKT signaling is deregulated numerous human malignancies, it become an attractive anticancer drug target. A number small molecule inhibitors have been developed; however, severe side effects prevented their use clinical trials. To find AKT1 with principally novel mechanism action, we carried out live cell-based screen for physical interaction between and its primary activator PDPK1....
The capability of spermatozoa to directly influence maternal gene expression is already established. Indeed, some the changes induced by may have a direct functional importance in pre-conceptional period. Although mechanisms underlying these sperm-maternal interactions are not well characterized, it possible that they could involve ligands released from spermatozoa. This study therefore aimed test whether physical contact between bovine and oviductal epithelial cells (BOECs) prerequisite for...
Abstract Cells with slow proliferation kinetics that retain the nuclear label over long time periods–the label-retaining cells (LRCs)–represent multipotent stem in a number of adult tissues. Since identity liver LRCs (LLRCs) had remained elusive we utilized genetic approach to reveal LLRCs normal non-injured livers and characterized their regenerative properties vivo culture. We found were located biliary vessels participated regeneration but not hepatocyte injury. In culture experiments...
Lgr5-LacZ mice harbor the Escherichia coli LacZ gene encoding β-galactosidase (β-gal) under control of Lgr5 promoter, a stem/progenitor cell marker. In injured livers mice, cells expressing are considered as potential bipotent liver progenitors; however, their origin and identity remain unknown. Unexpectedly, using lineage tracing, we demonstrate that β-gal+ do not originate from parenchymal cells. Instead, cells, isolated both wild-type positive for markers Kupffer liver-resident...