A5100392174- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Clostridium difficile and Clostridium perfringens research
- Circular RNAs in diseases
- Ginseng Biological Effects and Applications
- RNA Research and Splicing
- Gut microbiota and health
- Pharmacological Effects of Natural Compounds
- RNA modifications and cancer
- Antimicrobial Peptides and Activities
- Aquaculture Nutrition and Growth
- Invertebrate Immune Response Mechanisms
- Epigenetics and DNA Methylation
- Autophagy in Disease and Therapy
- Genomics, phytochemicals, and oxidative stress
- High Altitude and Hypoxia
- Heme Oxygenase-1 and Carbon Monoxide
- Barrier Structure and Function Studies
- Polysaccharides and Plant Cell Walls
- Histone Deacetylase Inhibitors Research
- Sesquiterpenes and Asteraceae Studies
- Viral gastroenteritis research and epidemiology
- Aquaculture disease management and microbiota
- Microbial infections and disease research
- Mycobacterium research and diagnosis
Third Hospital of Hebei Medical University
2022
Hebei Medical University
2022
Northwest A&F University
2022
The Fourth People's Hospital
2022
Gansu Agricultural University
2020-2021
Guangdong Ocean University
2018
XinHua Hospital
2018
Baoding People's Hospital
2018
Tongde Hospital of Zhejiang Province
2016
Yuhuangding Hospital
2016
Alzheimer's disease (AD) is the most common form of dementia among aging population. It pathologically characterized by synaptic impairment, accumulation neurofibrillary tangles and amyloid‑β (Aβ) deposition. MicroRNA‑26b (miR‑26b) has been observed to be upregulated in human temporal cortex AD, however, function miR‑26b not verified. Reverse transcription‑quantitative polymerase chain reaction was conducted investigate expression levels a double transgenic mouse model AD. Following...
Osteosarcoma (OS) is the most frequently occurring primary bone malignancy with a rapid progression and poor survival. In present study, in order to examine molecular mechanisms of OS, we analyzed microarray GSE28425. GSE28425 was downloaded from Gene Expression Omnibus, which also included miRNA expression profile, GSE28423, mRNA GSE28424. Each profiles 19 OS cell lines 4 normal bones. The differentially expressed genes (DEGs) miRNAs (DE-miRNAs) were screened using limma package...
This study explored the effect and mechanism of Rg3 on renal cell carcinoma (RCC) progression.RCC cells were treated with different concentrations Rg3, 5-Aza-dc (a methyltransferase inhibitor) or TSA deacetylase inhibitor). Rg3-induced cytotoxicity, migration, invasion, colony formation, tube formation apoptosis RCC evaluated by CCK-8, wound healing, Transwell, flow cytometry assays, respectively. Methylation expressions p53, p21 p16, methylation-related genes histone deacetylases...
Clostridium perfringens type C ( C. C) is one of the main microbial pathogens responsible for piglet diarrhea worldwide, causing substantial economic losses pig-rearing industries. The mitogen-activated protein kinase (MAPK) signaling pathway a key regulator inflammatory bowel disease, especially necrotic enteritis. However, whether and how MAPK involved in regulating process when challenged by are still unknown. Here, we screened 38 differentially expressed genes (DEGs) piglets’ ileum...
Clostridium perfringens beta2 (CPB2) toxin is one of the main pathogenic toxins produced by perfringens, which causes intestinal diseases in animals and humans. The N6-methyladenosine (m6A) modification most common reversible eukaryotic disease processes. Methyltransferase-like 3 (METTL3) regulates immunity inflammatory responses induced bacterial infections animals. However, METTL3′s involvement CPB2-treated porcine epithelial cell line-J2 (IPEC-J2) remains unclear. In current study, we...
Heme oxygenase 1 (HMOX1) is a stress-inducing enzyme with multiple cardiovascular protective functions, especially in hypoxia stress. However, transcriptional regulation of swine HMOX1 gene remains unclear. In the present study, we first detected tissue expression profiles adult Hezuo Tibetan pig and analyzed structure. We found that level was highest spleen pig, followed by liver, lung, kidney. A series 5’ deletion promoter plasmids pGL3-basic vector were used to identify core region...
microRNAs (miRNAs) can play a role in variety of physiological and pathological processes, their is achieved by regulating the expression target genes. Our previous high-throughput sequencing found that ssc-miR-185 plays an important regulatory piglet diarrhea, but its specific genes functions intestinal porcine epithelial cell (IPEC-J2) are still unclear. We intended to verify relationship between miR-185 division cycle 42 (CDC42) gene IPEC-J2 explore effect on proliferation cells.
Purpose: To study the influence of ginsenoside on apoptosis, cell cycle and autophagy in gastric carcinoma (GC), its effect reactive oxygen species (ROS) levels mitogen-activated protein kinase (MAPK) pathway. 
 Methods: Human cancer line BGC-823 was randomly divided into following groups: control, 100 μM (Rg5), 150 Rg5, 200 Rg5 groups. Western blot assay used to determine expressions autophagy-associated 12 (Atg12), Beclin-1, lc3b II, cycle-related protein, phosphated mitotic cyclin 25...
This study identified the biological role of miR-4728 in Burkitt lymphoma (BL) process. Ramos cells were used to analyze MicroRNA-4728 (miR-4728) functions. MiR-4728 expression was investigated 14 randomly chosen tumor tissues and 12 noncancerous by qRT-PCR. Cyquant assay monitor cell proliferation. Colony formation performed effectiveness on proliferation cells. The effects MAPK signaling pathway detected luciferase reporter assay. significance differences between groups evaluated SPSS. In...
N6-methyladenosine (m6A) modification can accommodate mRNA processing, stability, and translation in mammals, fat mass obesity associated protein (FTO) is a vital demethylase the m6A pathway. Clostridium perfringens type C (C. C) causes diarrhea piglets has serious impact on pig industry. However, our understanding of effect process C. infectious piglet (CPTCIPD) limited. Here, an vitro model CPTCIPD was constructed by treating intestinal porcine epithelial cell line-J2 (IPEC-J2) with beta2...