A5034314568- Animal Disease Management and Epidemiology
- Poxvirus research and outbreaks
- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- Rabies epidemiology and control
- Vector-Borne Animal Diseases
- Immunotherapy and Immune Responses
- T-cell and Retrovirus Studies
- Viral gastroenteritis research and epidemiology
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Toxin Mechanisms and Immunotoxins
- Animal Virus Infections Studies
- vaccines and immunoinformatics approaches
- Viral Infectious Diseases and Gene Expression in Insects
- Virology and Viral Diseases
- CAR-T cell therapy research
- Microbial infections and disease research
- NF-κB Signaling Pathways
- Viral Infections and Immunology Research
- Milk Quality and Mastitis in Dairy Cows
- Cytokine Signaling Pathways and Interactions
- Plant and Fungal Interactions Research
- Transgenic Plants and Applications
- Natural product bioactivities and synthesis
University of Southampton
2009-2023
Centre for Cancer Biology
2023
Imperial College London
2011
Southampton General Hospital
2008-2009
Cancer Research UK
2009
University of Liège
1990-2006
Physiol (Belgium)
2004-2006
Newbury College
2002
Université Claude Bernard Lyon 1
2000
Centre National de la Recherche Scientifique
2000
The intracellular trafficking of major histocompatibility complex class I (MHC-I) proteins is directed by three quality control mechanisms that test for their structural integrity, which correlated to the binding high-affinity antigenic peptide ligands. To investigate molecular features MHC-I these detect, we have followed hypothesis suboptimally loaded are characterized conformational mobility in F pocket region site. We created a novel variant an protein, Kb-Y84C, two alpha helices this...
ABSTRACT The immunodominant, 39,000-molecular weight core protein (39K protein) of fowlpox virus (FP9 strain), equivalent to the vaccinia A4L gene product, contains highly charged domains at each end and multiple copies a 12-amino-acid serine-rich repeat sequence in middle protein. Similar repeats were also detected other strains, suggesting that they might confer selective advantage virus. molloscum contagiosum homolog (MC107L) repeats, unlike number does not seem be crucial, since some...
MHC-I molecules play a central role in the immune response to viruses and cancers. They present peptides on surface of affected cells, for recognition by cytotoxic T cells. Determining which are presented, what proportion, has profound implications developing effective, medical treatments. However, our ability predict peptide presentation levels is currently limited. Existing prediction algorithms focus primarily binding affinity MHC-I, do not relative abundance individual antigen presenting...
Release of fowlpox virus (FWPV) as extracellular enveloped (EEV) appears to proceed both by the budding intracellular mature (IMV) through plasma membrane and fusion (IEV) with membrane. Based on frequency events compared wrapping observed electron microscopy, FWPV FP9 strain seems exit chick embryo fibroblast cells predominantly budding. In contrast vaccinia (VV), production particles is not affected N 1-isonicotinoyl-N 2-3-methyl-4-chlorobenzoylhydrazine (IMCBH). Comparison sequence VV...
Background CD8 + T cells are a highly diverse population of with distinct phenotypic functions that can influence immunotherapy outcomes. Further insights on the roles specificities and TCR avidity naturally arising tumor-specific cells, where both high low recognizing same peptide-major histocompatibility complex (pMHC) coexist in tumor, crucial for understanding cell exhaustion resistance to PD-1 immunotherapy. Methods CT26 models were treated anti-PD-1 days 3, 6 9 following subcutaneous...
Several fox vaccination campaigns against rabies have been undertaken in Belgium by using a vaccinia‐rabies recombinant virus distributed baits the field. However, foxes and other wild animals that may ingest could be infected at same time another orthopoxvirus, such as cowpox virus, which circulates wildlife. Recombination between two viruses therefore occur. A serological survey for antibodies to particularly was several species. Antibodies were detected only rodent species, 16 of 25 bank...
Twenty-one monoclonal antibodies (MAbs) directed against the NY-1 and Osloss-c strains of bovine viral diarrhoea virus were produced characterized by indirect immunofluorescence assay, radioimmuno-precipitation neutralization tests. Fourteen MAbs strain recognized gp48 showed a weak neutralizing activity in presence goat anti-mouse immunoglobulin serum.
Abstract Tapasin edits the peptide repertoire presented to CD8+ T cells by favoring loading of slow off-rate peptides on MHC I molecules. To investigate role tapasin cell immunodominance we used poxvirus viral vectors expressing a polytope lymphocytic choriomeningitis virus epitopes with different off-rates. In tapasin-deficient mice, responses subdominant fast were clearly favored. This alteration hierarchy was consequence editing and not in because bone marrow chimeric mice (wild-type...
SUMMARY Bovine herpesvirus type 4 proteins were identified by PAGE of [35S]methionine- or [3H]glucosamine-labelled purified virions. Thirty-one monoclonal antibodies (MAbs) raised against the V. Test strain used to identify 29 proteins, ten which glycosylated. All these glycoproteins belonged viral envelope and a 140K nonglycosylated protein appeared be major nucleocapsid protein. The MAbs classified into two groups. first group precipitated three M r 150K, 120K 51K. 51K linked disulphide...
Abstract In the CT26 BALB/c murine model of colorectal carcinoma, depletion regulatory T cells (Tregs) prior to tumor inoculation results in protective immunity both and other BALB/c-derived tumors diverse histological origin. this paper, we show that cross-protection can be conferred by adoptively transferred CD8+ CTLs. Other schedules for inducing have been described, but they do not lead cross-protection. We Treg ablation facilitates development new CTL specificities are normally cryptic,...
ABSTRACT Genes encoding fowlpox virus (FWPV) structural proteins have been identified mainly by sequence homology with those from vaccinia (VACV), but little is known about the encoded proteins. Production of monoclonal antibodies (MAbs) against Poxine and HP1-440 (Munich) clone FP9 allowed identification three immunodominant FWPV proteins: 39-kDa core protein (encoded FPV168, homologous to VACV A4L), a 30- 35-kDa doublet, an abundant 63-kDa protein. The are nonglycosylated, antigenically...
In infected cattle, bovine herpesvirus type 4 (BHV-4) induces an immune response with low neutralizing antibody levels or in the absence of such antibodies. For study this phenomenon, monoclonal antibodies (MAbs) raised against two BHV-4 glycoproteins identified previously (150K7120K/51K and 120K/16·5K) were used neutralization tests. None MAbs except for MAb 16 could neutralize alone; pairs 150K/120K/51K 120K/16·5K able to infectivity. involved competitive binding assays identify epitopes...
Modified vaccinia virus Ankara (MVA) is an attenuated strain derived from (VV) that grows efficiently in primary chicken embryo fibroblasts (CEFs) and baby hamster kidney cells only. MVA produces significantly more of the enveloped forms VV infected CEFs compared with Copenhagen. In present study, production different infectious was or two well-characterized replication-competent strains, WR IHD-J. a time-course experiment, infectivity associated extracellular (EEV), cell-associated (CEV)...
To improve both safety and stability of the vaccines used in field to vaccinate foxes against rabies by oral route, a recombinant vaccinia virus, expressing glycoprotein virus (VVTGgRAB) has been developed. VVTGgRAB innocuity was verified target species domestic animals as well numerous wild animal that could compete with red fox consuming vaccine baits Europe. Oral immunization foxes, distributing vaccine-baits, undertaken for whole infected area Belgium (10,000 km2). Five campaigns...
Tapasin, a component of the major histocompatibility complex (MHC) I peptide loading complex, edits repertoire peptides that is presented at cell surface by MHC and thereby plays key role in shaping hierarchy CD8+ T-cell responses to tumors pathogens. We have developed system allows us tune level tapasin expression independently regulate competing different off-rates. By quantifying relative molecules, we show editing can be measured terms “tapasin bonus,” which dependent on both kinetic...