A5041592939- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Enzyme Structure and Function
- Mass Spectrometry Techniques and Applications
- Plant and animal studies
- Analytical Chemistry and Chromatography
- Amino Acid Enzymes and Metabolism
- Metabolism and Genetic Disorders
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Cancer, Hypoxia, and Metabolism
- Biochemical and Molecular Research
- Plant Parasitism and Resistance
- Plant biochemistry and biosynthesis
- Isotope Analysis in Ecology
- Insect and Pesticide Research
- Advanced Chemical Sensor Technologies
- Microbial Metabolic Engineering and Bioproduction
- Plant nutrient uptake and metabolism
- Enzyme Catalysis and Immobilization
- Insect and Arachnid Ecology and Behavior
- Mitochondrial Function and Pathology
- Plant Stress Responses and Tolerance
- Porphyrin Metabolism and Disorders
University of Minnesota
2016-2025
University of Minnesota System
2009-2016
Plant (United States)
2014
University of Wisconsin–Madison
1997-2013
Twin Cities Orthopedics
2013
Minnesota Wire (United States)
2009
Ames Research Center
2005
National Aeronautics and Space Administration
2005
University of Nevada, Reno
2004
Massachusetts General Hospital
2004
Plant secondary metabolites (PSMs) are a key mechanism by which plants defend themselves against potential threats, and changes in the abiotic environment can alter diversity abundance of PSMs. While number studies investigating effects factors on PSM production is growing, we currently have limited understanding how combinations may influence production. The objective this study was to determine warming influences addition other modulate effect. We used untargeted metabolomics evaluate five...
Global warming poses a threat to plant survival, impacting growth and agricultural yield. Protein turnover, critical regulatory mechanism balancing protein synthesis degradation, is crucial for the cellular response environmental changes. We investigated effects of elevated temperature on proteome dynamics in Arabidopsis thaliana seedlings using 15N-stable isotope labeling ultra-performance liquid chromatography-high resolution mass spectrometry, coupled with ProteinTurnover algorithm....
Using a maskless photolithography method, we produced DNA oligonucleotide microarrays with probe sequences tiled throughout the genome of plant Arabidopsis thaliana. RNA expression was determined for complete nuclear, mitochondrial, and chloroplast genomes by tiling 5 million 36-mer probes. These probes were hybridized to labeled mRNA isolated from liquid grown T87 cells, an undifferentiated cell culture line. Transcripts detected at least 60% nearly 26,330 annotated genes, which included...
Abstract We report the first metabolic labeling of Arabidopsis thaliana for proteomic investigation, demonstrating efficient and complete intact plants. Using a reversed‐database strategy, we evaluate performance MASCOT search engine in analysis combined natural abundance 15 N‐labeled samples. find that N‐metabolic appears to increase ambiguity associated with peptide identifications due part changes number isobaric amino acids when isotopic label is introduced. This reflected by...
We hypothesised that seasonal pasture myopathy (SPM), which closely resembles atypical (AM), was caused by ingestion of a seed-bearing plant abundant in autumn pastures.To identify common among pastures horses with SPM, and to determine whether the toxic amino acid hypoglycin A present seeds metabolites were SPM horse serum or urine.Twelve cases, 11 23 control farms visited all autumn. seed analysed for composition (n = 7/7) GC-MS its metabolite 4/4) identified conjugated form [tandem mass...
In natural systems plants face a plethora of antagonists and thus have evolved multiple defence strategies. Lima bean (Phaseolus lunatus L.) is model plant for studies inducible indirect anti-herbivore defences including the production volatile organic compounds (VOCs) extrafloral nectar (EFN). contrast, on direct chemical mechanisms as crucial components lima beans' syndrome under conditions are nonexistent. this study, we focus cyanogenic potential (HCNp; concentration glycosides)...
Summary 1. Plants express multiple defensive traits, but little is known about the genetic stability and phenotypic plasticity of these traits in nature. To investigate sources variation their potential ecological consequences for herbivores, we combined field observations cyanogenic lima bean with laboratory experiments. 2. Field studies South Mexico revealed a distinct variability within among wild populations. differentiate impact ambient conditions on plant phenotypes, used seed‐grown...
The deposition of antimicrobial plant resins in honey bee, Apis mellifera, nests has important physiological benefits. Resin foraging is difficult to approach experimentally because resin composition highly variable among and between families, the environmental plant-genotypic effects on are unknown, foragers relatively rare often forage unobservable tree canopies. Subsequently, little known about botanical origins many regions or benefits specific bees. We used metabolomic methods as a type...
Temperature, water, and light are three abiotic stress factors that have major influences on plant growth, development, reproduction. Plants can be primed by a prior mild to enhance their resistance future stress. We used an untargeted metabolomics approach examine Arabidopsis thaliana 11-day-old seedling's responses including heat (with without priming), cold water-deficit high-light before after 2-day-recovery period. Analysis of the physiological phenotypes showed seedlings with treatment...
A prevalent side-reaction of succinate dehydrogenase oxidizes malate to enol-oxaloacetate (OAA), a metabolically inactive form OAA that is strong inhibitor dehydrogenase. We purified from cow heart mitochondria an enzyme (OAT1) with tautomerase (OAT) activity converts enol-OAA the physiological keto-OAA form, and determined it belongs highly conserved previously uncharacterized Fumarylacetoacetate_hydrolase_domain-containing protein family. From all three domains life, heterologously...
UDP-galactose 4-epimerase from Escherichia coli catalyzes the interconversion of and UDP-glucose through transient reduction tightly bound cofactor NAD+. The enzyme is unique among NAD+-dependent enzymes in that it promotes stereospecific but nonstereospecific hydride return during normal catalysis. In addition to transfer, reaction mechanism epimerase involves two key features: abstraction a proton 4'-hydroxyl group glucose or galactose by an active site base rotation 4-ketopyranose...
Abstract A salinity and dehydration stress-responsive calcium-dependent protein kinase (CDPK) was isolated from the common ice plant (Mesembryanthemum crystallinum; McCPK1). McCPK1 undergoes myristoylation, but not palmitoylation in vitro. Removal of N-terminal myristate acceptor site partially reduced plasma membrane (PM) localization as determined by transient expression green fluorescent fusions microprojectile-bombarded cells. domain (amino acids 1–70) completely abolished PM...
Abstract For the last 50 years, scientists have recognized that varying ratios of plant hormones cytokinin and auxin induce cells to form particular tissues: undifferentiated calli, shoot structures, root or a whole plant. Proliferation callus tissue, greening, formation structures are all cytokinin-dependent processes. Habituation refers naturally occurring phenomenon whereby cultures, upon continued passage, lose their requirement for cytokinin. Earlier studies calli with...
Typical mass spectrometry-based protein lists from purified fractions are confounded by the absence of tools for evaluating contaminants. In this report, we compare results a standard survey experiment using an ion trap spectrometer with those obtained dual isotope labeling and Q-TOF to quantify degree enrichment proteins in subcellular Arabidopsis plasma membrane. Incorporation stable isotope, either H218O or H216O, during trypsinization allowed relative quantification within membranes...
Assignment of individual compound identities within mixtures thousands metabolites in biological extracts is a major challenge for metabolomic technology. Mass spectrometry offers high sensitivity over large dynamic range abundances and molecular weights but limited its capacity to discriminate isobaric compounds. In this article, we have extended earlier studies using isotopic labeling elemental composition elucidation (Rodgers, R. P.; Blumer, E. N.; Hendrickson, C. L.; Marshall, A. G. J....
The plant hormone auxin, indole-3-acetic acid (IAA), plays important roles in growth and development. signaling response to IAA is largely dependent on the local concentration of IAA, this regulated by multiple mechanisms plants. Therefore, precise quantification provides insights into regulation its biological roles. Meanwhile, pathways genes involved biosynthesis are not fully understood, so it necessary analyze production at metabolite level for unbiased studies biosynthesis.We have...