A5055688170- Genetic Mapping and Diversity in Plants and Animals
- Peroxisome Proliferator-Activated Receptors
- Synthesis and Biological Evaluation
- Pancreatic function and diabetes
- Adipose Tissue and Metabolism
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Cardiac electrophysiology and arrhythmias
- Lipid metabolism and biosynthesis
- Gene expression and cancer classification
- RNA Research and Splicing
- Diabetes Treatment and Management
- Genetic diversity and population structure
- Cancer Research and Treatments
- Eicosanoids and Hypertension Pharmacology
- Receptor Mechanisms and Signaling
- Endoplasmic Reticulum Stress and Disease
- Genomics and Phylogenetic Studies
- MicroRNA in disease regulation
- Metabolism, Diabetes, and Cancer
- RNA modifications and cancer
- Cardiovascular Function and Risk Factors
- Nitric Oxide and Endothelin Effects
- Peptidase Inhibition and Analysis
- Regulation of Appetite and Obesity
Yanbian University
2013-2025
Dalian Medical University
2025
Chongqing Medical University
2025
Second Affiliated Hospital of Chongqing Medical University
2025
Hanoi Medical University
2024
Nanjing University
2024
Hainan Medical University
2021-2023
Hainan General Hospital
2018-2023
Fujian Agriculture and Forestry University
2022
Jianghan University
2021
Stearoyl–CoA desaturase (SCD) is a central lipogenic enzyme catalyzing the synthesis of monounsaturated fatty acids, mainly oleate (C18:1) and palmitoleate (C16:1), which are components membrane phospholipids, triglycerides, wax esters, cholesterol esters. Several SCD isoforms ( SCD1 - 3 ) exist in mouse. Here we show that mice with targeted disruption isoform have reduced body adiposity, increased insulin sensitivity, resistant to diet-induced weight gain. The protection from obesity...
Abstract DNA microarrays provide for unprecedented large‐scale views of gene expression and, as a result, have emerged fundamental measurement tool in the study diverse biological systems. Statistical questions abound, but many traditional data analytic approaches do not apply, large part because thousands individual genes are measured with relatively little replication. Empirical Bayes methods natural approach to microarray analysis they can significantly reduce dimensionality an inference...
Lead (Pb), a heavy metal environmental pollutant, poses threat to the health of humans and birds. Inflammation is one most common pathological phenomena in case illness poisoning. However, underlying mechanisms inflammation remain unclear. The cerebellum thalamus are important parts nervous system. To date, there have been no reports Pb inducing animal cerebellums or thalami. Selenium (Se) can relieve Therefore, we aimed explore mechanism by which Se alleviates toxicity thalami chickens...
G protein-coupled receptor 119 (GPR119) is expressed in pancreatic islets and intestine, involved insulin incretin hormone release. GPR119-knockout (Gpr119(-/-)) mice were reported to have normal islet morphology size, body weight (BW), fed/fasted glucose levels. However, the physiological function of GPR119 its role maintaining homeostasis under metabolic stress remain unknown. Here, we report phenotypes an independently generated line Gpr119(-/-) basal high-fat diet (HFD)-induced obesity....
The lipogenic gene stearoyl-CoA desaturase (SCD)1 appears to be a promising new target for obesity-related diabetes, as mice deficient in this enzyme are resistant diet- and leptin deficiency-induced obesity. BTBR mouse strain replicates many features of insulin resistance found humans with excess visceral adiposity. Using the hyperinsulinemic-euglycemic clamp technique, we determined that sensitivity was improved heart, soleus muscle, adipose tissue, liver SCD1-deficient mice. We next...
Summary Traditional genetic mapping has largely focused on the identification of loci affecting one, or at most a few, complex traits. Microarrays allow for measurement thousands gene expression abundances, themselves traits, and number recent investigations have considered these measurements as phenotypes in studies. Combining traditional quantitative trait (QTL) methods with microarray data is powerful approach demonstrated utility biological investigations. These (eQTL) studies are...
Coordinated regulation of gene expression levels across a series experimental conditions provides valuable information about the functions correlated transcripts. The consideration correlation over time or tissue dimension has proved in predicting function. Here, we consider correlations genetic dimension. In addition to identifying coregulated genes, also supplies us with genomic locations putative regulatory loci. We calculated among approximately 45,000 traits derived from 60 individuals...
Obesity is a strong risk factor for the development of type 2 diabetes. We have previously reported that in adipose tissue obese (ob/ob) mice, expression adipogenic genes decreased. When made genetically obese, BTBR mouse strain diabetes susceptible and C57BL/6J (B6) resistant. used DNA microarrays RT-PCR to compare gene BTBR-ob/ob versus B6-ob/ob mice tissue, liver, skeletal muscle, pancreatic islets. Our results show: 1) there an increased involved inflammation diabetic mice; 2) lipogenic...
FFAR1/GPR40 is a G-protein-coupled receptor expressed predominantly in pancreatic islets mediating free fatty acid-induced insulin secretion. However, the physiological role of FFAR1 remains controversial. It was previously reported that knockout (Ffar1(-/-)) mice were resistant to high-fat diet-induced hyperinuslinemia, hyperglycemia, hypertriglyceridemia, and hepatic steatosis. A more recent report suggested although necessary for secretion vivo, deletion did not protect against islet...
GPR119 is a G protein-coupled receptor that preferentially expressed in islet cells and mediates insulin secretion. Oleoyl-lysophosphatidylcholine oleoylethanolamide (OEA) act as endogenous ligands for this receptor, whereas PSN375963 PSN632408 are two recently reported synthetic agonists. In study, we explored mechanisms underlying GPR119-induced addition, assessed the potential utility of agonists tools exploring biology.We examined natural agonist activity at MIN6c4 RINm5f insulinoma...
The G protein-coupled receptor 119 (GPR119) mediates insulin secretion from pancreatic β cells and glucagon-like peptide 1 (GLP-1) release intestinal L cells. While GPR119-mediated is glucose dependent, it not clear whether or GLP-1 similarly requires glucose. This study was designed to address the glucose-dependence of secretion, explore cellular mechanisms hormone in versus those cells.GLP-1 response GPR119 agonists ion channel modulators, with without glucose, analysed cell line GLUTag,...
Fatty acid binding protein-4 (FABP4) and FABP5 are two closely related FA proteins expressed primarily in adipose tissue and/or macrophages. The small-molecule FABP4 inhibitor BMS309403 was previously reported to improve insulin sensitivity leptin-deficient Lep(ob)/Lep(ob) (ob/ob) mice. However, this compound not extensively characterized the more physiologically relevant animal model of mice with diet-induced obesity (DIO). Here, we report discovery characterization a novel series FABP4/5...
We previously reported that mice deficient in stearoyl-CoA desaturase-1 (Scd1) and maintained on a very low-fat (VLF) diet for 10 days developed severe loss of body weight, hypoglycemia, hypercholesterolemia, many cholestasis-like phenotypes. To better understand the metabolic changes associated with these phenotypes, we performed microarray analysis hepatic gene expression chow- VLF-fed female Scd1+/+ Scd1-/- mice. identified an extraordinary number differentially expressed genes (>4,000...
G-protein-coupled bile acid receptor 1 (GPBAR1/TGR5/M-Bar/GPR131) is a cell surface involved in the regulation of metabolism. We have previously shown that Gpbar1 -null mice are resistant to cholesterol gallstone disease when fed lithogenic diet. Other published studies suggested both energy homeostasis and glucose homeostasis. Here, we examine functional role diet-induced obese mice. found body weight, food intake, fasted blood levels were similar between their wild-type (WT) littermates...
Proprotein convertase subtilisin/kexin type 9 (PCSK9) induces degradation of low-density lipoprotein receptor (LDLR) in the liver. It is being pursued as a therapeutic target for LDL-cholesterol reduction. Earlier genome-wide gene expression studies showed that PCSK9 over-expression HepG2 cells resulted up-regulation genes cholesterol biosynthesis and down-regulation stress response pathways; however, it was not known whether these changes were directly regulated by or secondary to...
Circular RNAs (circRNAs) are increasingly recognized as important regulators in cancer including ovarian (OC). This work focuses on the effects of circ_0000745 OC development and molecules involved.Expression collected tissues acquired cell lines was examined by RT-qPCR. The stability cells RNase R treatment. target transcripts interacted with were predicted using bioinformatic systems. Gain- loss-of-function studies circ_0000745, microRNA (miR)-3187-3p erb-b2 receptor tyrosine kinase 4...
Abstract We have used a rat model of induced mammary carcinomas in an effort to identify breast cancer susceptibility genes. Using genetic crosses between the carcinoma-resistant Copenhagen (COP) and carcinoma-sensitive Wistar-Furth rats, we confirmed identification Mcs1 locus that modulates tumor number. now also identified two additional loci, Mcs2 Mcs3. These three loci map chromosomes 2, 7, 1, respectively, interact additively suppress carcinoma development COP strain. They are...
BACKGROUND: Aberrant expression of long non-coding RNAs is involved in the progression ovarian cancer. However, clinical significance and biological functions SNHG3 was little known cancer (OC). METHODS: The tissues paired adjac ent normal detected using quantitative real time polymerase chain reaction (qRT-PCR). Gain-of function loss-of assays were performed cells to demonstrate effects on cell proliferation invasion. relative protein levels determined western blot analyses. RESULTS:...
Pancreatic cancer is the fourth leading cause of cancer-associated mortality in developed countries. ductal adenocarcinoma (PDAC) accounts for ~90% all pancreatic cases. The Notch signaling pathway serves a crucial role embryonic development, as well during tumorigenesis different types cancer. However, either oncogenic or tumor suppressor roles depending on tissue type. There are four receptors (Notch1-4) and five ligands [Jagged1, Jagged2, δ-like ligand protein (DLL)1, DLL3 DLL4];...
Tumor-promotive tumor-associated macrophages (TAMs) and the CXCL16/CXCR6 axis have been reported to be correlated with limited efficacy of chemotherapy in ovarian cancer (OC). However, role TAM-secreted CXCL16 mechanism by which it affects cisplatin (DDP) resistance OC cells remain elusive.We induced human THP-1 monocytes differentiate into macrophages. Next, SKOV3 TOV-112D were co-cultured macrophages, followed incubation increasing concentrations DDP. The effects CXCL16, CXCR6, WTAP on DDP...