Context: Plants and most of the plant-derived compounds have long been known for their potential pharmaceutical effects. They are well to play an important role in treatment several diseases from diabetes various types cancers. Today clinically effective pharmaceuticals developed ancestors history medicine.Objective: The aim this study was evaluate free radical scavenging activity total phenolic flavonoid contents methanol, ethanol, acetone extracts flowers leaves Onopordum acanthium L.,...

Abstract OBJECTIVE: To identify differences in the expression of certain structural proteins and angiogenic growth factors vessel tissues that represent different phases process intracranial aneurysm formation rupture: normal wall, intact (unruptured) ruptured wall. METHODS: The novel study design involved 10 pairs specimens (ruptured unruptured wall) obtained perioperatively during clipping operations patients with multiple aneurysms. All surgeries were performed within 5 days subarachnoid...

Recent studies have demonstrated the utility of recombinant adeno-associated viral (rAAV) vectors in generation human knockout cell lines. The efficiency with which such lines can be generated using rAAV, comparison more extensively described plasmid-based approaches, has not been directly tested. In this report, we demonstrate that targeting constructs delivered by rAAV were nearly 25-fold efficient than transfected plasmids target same exon. addition, describe a novel vector configuration...

To evaluate the relationship between serum oxidant-antioxidant balance and presence of exfoliation syndrome (XFS) in a prospective study.The study group comprised 27 cases XFS. Serum concentrations myeloperoxidase, malondialdehyde, vitamins A, C E, catalase total antioxidant were measured compared with an independent by t-test.Serum A parameters not statistically different among groups (P >0.05). vitamin significantly lower malondialdehyde higher XFS when control (P=0.008 P=0.002,...

Dynamic exchange of a subset nucleosomes in vivo plays important roles epigenetic inheritance chromatin states, insulator function, chromosome folding, and the maintenance pluripotent state embryonic stem cells. Here, we extend pulse-chase strategy for carrying out genome-wide measurements histone dynamics to several variants murine cells somatic tissues, recapitulating expected characteristics well characterized H3.3 variant. We extended this system less-studied MacroH2A2 variant, commonly...

This study is based on the phenolic composition and antioxidant, anticancer, antimicrobial, antibiofilm activities of edible mushroom Marasmius oreades from Turkey. The an M. ethanol extract was measured by using Folin-Ciocalteu method, aluminium chloride colorimetry, ultraperformance liquid chromatography. antioxidant activity evaluated basis DPPH radical scavenging activity. effect also screened in order to determine glutathione-S-transferase, glutathione peroxidase, catalase, superoxide...

Cisplatin (CP) is a chemotherapeutic agent used to treat various types of cancer; nephrotoxicity the most common adverse effect drug. We investigated protective effects propolis against CP induced kidney injury. Thirty-six male rats were divided into six equal groups: untreated control group, 50 mg/kg/day 100 single-dose 7 mg/kg + and propolis. Rats sacrificed after 14 days kidneys removed for histopathological biochemical analyses. hematoxylin & eosin periodic acid-Schiff staining evaluate...

Effects of cobalt on the antioxidant status control and streptozotocin diabetic rat heart aorta were examined at second, fourth sixth week treatment. Rats divided into four groups: control, diabetic, treated with chloride chloride. Diabetes was induced by tail vein injection (STZ). Cobalt treatment groups given 0.5 mM CoCl(2) in drinking water. The rats both further subdivided three six each. these subgroups studied 2-week intervals up to 6 weeks. At end experiment, all animals sacrificed...

This experimental study assessed the usefulness of a rat model corneal angiogenesis for assessing angiogenic activity cerebrovascular malformations (CVMs) over time. It is first investigation to have focused on dynamic quantification related CVMs (i.e., changes time as opposed one point in time) and compared different other tissues this way.The CVM specimens were from 15 randomly selected arteriovenous malformation (AVM) cases, cavernous 2 venous angioma cases. All samples came surgical...

Background: The aim was to assess wound healing when parenteral fish‐oil emulsion is given rats receiving dexamethasone. Methods: For 5 days after skin wounding, group S (control; n = 7) received saline 1 mL/kg intraperitoneal (IP); D (n 7), dexamethasone 0.2 mg/kg IP; and DO 9), IP plus Omegaven (Fresenius Kabi, Austria). Wound specimens were assessed for hydroxyproline level, depth, histology (epidermal/dermal regeneration, granulation tissue thickness, angiogenesis), expression of...

Ethanolic extracts of the mushroom species Ganoderma adspersum, Inonotus hispidus, Russula chloroides, and Sarcodon imbricatus were investigated for their polyphenolic contents biological activities.The radical scavenging activity was evaluated by 2,2-diphenyl-1-(2,4,6-trinitrophenyl) (DPPH) method compounds determined high performance liquid chromatography (HPLC) analysis. Furthermore, effects on enzyme glutathione-S-transferase (GST) also examined. Additionally, antimicrobial disc...

Purpose To elucidate whether the gene polymorphisms of glutathione S-transferase (GST) M1, T1, and PI are associated with development exudative age-related macular degeneration. Methods The authors genotyped 35 white patients degeneration 159 healthy controls. Genomic DNA from peripheral blood was examined using polymerase chain reaction defined for genetic GST. Results No association observed between GSTM1, GSTT1, GSTP1 risk (p>0.05). frequencies combination GSTM1 (null) (mutant),...

Background. Age-related cataract (ARC) is the leading cause of visual disability and reversible blindness all over world. The different expressions GST isozymes among animals may explain variations in formation caused by oxidative stress.Objectives. In this study, we evaluated distribution gene polymorphisms ARC patients possible associations between presence polymorphisms.Material Methods. epidemiological data was collected a standard questionnaire blood samples were obtained from 130 159...

To assess the safety and efficacy of a gemcitabine plus docetaxel regimen as second line therapy for patients with advanced soft tissue sarcoma (STS) resistant to doxorubicin ifosfamide-based therapy.Medical records 64 STS who received treatment between May 2006 June 2011 were examined. All had been previously treated at first setting. Patients 900 mg/m2 on days one eight intravenously over 90 minutes, followed by 75 day hour. Cycles repeated every 3 weeks.The male-to-female ratio was 37/27...

Many proteins that are needed for progression through S-phase produced from transcripts peak in the S-phase, linking temporal expression of those to time they required cell cycle. Here, we explore potential roles long non-coding RNAs cycle progression. We use a sensitive click-chemistry approach isolate nascent human line, and identify more than 900 (lncRNAs) whose synthesis peaks during S-phase. More 200 these intergenic (lincRNAs) with S-phase-specific expression. characterize three...

The antioxidant capacity of Fomitopsis pinicola extracts was determined based on DPPH radical scavenging activites. Their total phenolic and flavonoid contents were also investigated. Antioxidant enzymes are important in controlling oxidative stress maintaining cellular homeostatis. They can protect cells from the deleterious effects reactive oxygen species prevent development degenerative diseases such as Alzhemier disease, Parkinson cancer, even aging. In this regard, modulatory activities...

In the present study, liver, lung, heart and kidney superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) activities, lipid peroxidation, nitrite vitamin C levels were investigated in diabetic rats. Diabetes induced rats by streptozotocin (STZ) treated received 1 g/l with 0.5 mM CoCl2 drinking water for eigth weeks. all tissues, dismutase, peroxidase, peroxidation significantly increased at end of 8th week (p < 0.05) whereas level was decreased compared to those...