A5056578272- Photonic and Optical Devices
- Advanced Biosensing Techniques and Applications
- Mechanical and Optical Resonators
- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Sensors
- Innovative Microfluidic and Catalytic Techniques Innovation
- Monoclonal and Polyclonal Antibodies Research
- 3D Printing in Biomedical Research
- Microfluidic and Capillary Electrophoresis Applications
- Electrowetting and Microfluidic Technologies
- Photonic Crystals and Applications
- Advanced Fiber Optic Sensors
- Planetary Science and Exploration
- Nanofabrication and Lithography Techniques
- Lipid Membrane Structure and Behavior
- Acoustic Wave Resonator Technologies
- Force Microscopy Techniques and Applications
- Nanopore and Nanochannel Transport Studies
- Collagen: Extraction and Characterization
- Bone Tissue Engineering Materials
- Advanced Fluorescence Microscopy Techniques
- Biosensors and Analytical Detection
- Cellular Mechanics and Interactions
- Single-cell and spatial transcriptomics
- X-ray Diffraction in Crystallography
University of Michigan
2016-2025
University of Hawaii System
2025
Fudan University
2023
The University of Texas at El Paso
2023
Stockholm University
2023
Curtin University
2023
Michigan State University
2023
Rutherford Appleton Laboratory
2023
Confederazione Nazionale dell'Artigianato e Della Piccola e Media Impresa
2023
Columbia University
2023
We report the synthesis of new "branched" gold nanocrystals in high yield (over 90%) via a wet-chemical route. The branched exhibit shape-dependent plasmon resonance that is red-shifted by 130−180 nm from spherical particle wavelength. Discrete dipole approximation (DDA) calculations qualitatively replicate observed optical extinction spectra nanocrystals, indicating surface mainly determined in-plane excitation associated with sharp tips.
A platform for performing rapid, real-time binding assays on sensor arrays based silicon ring resonators is presented in this paper. An array of 32 sensors interrogated simultaneously. Using eight as controls, 24 simultaneous curves are produced. The bulk refractive index sensitivity the system was demonstrated down to 7.6 × 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-7</sup> and sensor-to-sensor variability 3.9%. an 8-min incubation,...
Recent advances in label-free biosensing techniques have shown the potential to simplify clinical analyses. With this motivation mind, paper demonstrates for first time use of silicon-on-insulator microring optical resonator arrays robust and detection a clinically important protein biomarker undiluted serum, using carcinoembryonic antigen (CEA) as test case. We utilize an initial-slope-based quantitation method sensitively detect CEA at relevant levels determine concentrations unknown...
We present a combination of theory and experiment designed to elucidate the properties gold nanoshells. Wet chemistry methods are used prepare nanoshells, transmission electron microscopy (TEM) analysis is characterize shell structure, demonstrating presence pinholes in shells. Both Mie discrete dipole approximation (a numerical method) electrodynamics structures, including both perfect pinhole defected The calculations show that 2−5 nm have only small effect on extinction spectra; however,...
Whether for pathological examination or fundamental biology studies, different classes of biomaterials and biomolecules are each measured from a region typically heterogeneous tissue sample, thus introducing unavoidable sources noise that hard to quantitate. We describe the method DNA-encoded antibody libraries (DEAL) spatially multiplexed detection ssDNAs proteins as well cell sorting, all on same diagnostic platform. DEAL is based upon coupling ssDNA oligomers onto antibodies which then...
Microrings for microRNAs! A label-free method has been developed the sensitive detection of microRNAs utilizing arrays silicon photonic microring resonators. This simple and modularly multiplexable direct profiling microRNA within 10 minutes meets a number challenges faced by current methodologies in this area. Detailed facts importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted...
A general method for the non-oxidative functionalization of single-crystal silicon(111) surfaces is described. The silicon surface fully acetylenylated using two-step chlorination/alkylation chemistry. benzoquinone-masked primary amine attached to this via Cu(I)-catalyzed Huisgen 1,3-dipolar cycloaddition ("click" chemistry). benzoquinone electrochemically reduced, resulting in quantitative cleavage molecule and exposing terminus. Molecules presenting a carboxylic acid have been immobilized...
In this paper, we present a method for the sensitive detection of microRNAs (miRNAs) utilizing an antibody that specifically recognizes DNA:RNA heteroduplexes and silicon photonic microring resonator array transduction platform. Microring arrays are covalently functionalized with DNA capture probes complementary to solution phase miRNA targets. Following hybridization on sensor, anti-DNA:RNA is introduced binds selectively heteroduplexes, giving larger signal than original due increased mass...
We report a real-time DNA detection method that utilizes single-strand DNA-modified nanoparticle probes and micropatterned chemoresponsive diffraction gratings interrogated simultaneously at multiple laser wavelengths. The surface-bound probe based assay with the grating signal transduction scheme results in an experimentally simple protocol, displaying attributes of both methodologies: high sensitivity selectivity afforded by experimental simplicity, wavelength-dependent resonant...
The ability to perform multiple simultaneous protein biomarker measurements in complex media with picomolar sensitivity presents a large challenge disease diagnostics and fundamental biological studies. Silicon photonic microring resonators represent promising platform for real-time detection of biomolecules on account their spectral toward surface binding events between target antibody-modified microrings. For all refractive index-based sensing schemes, the mass bound analytes, combination...
We demonstrate a three-step assay on silicon photonic microring resonator-based detection platform that enables the quantitation of cardiac biomarker C-reactive protein (CRP) over dynamic range spanning six orders magnitude. Using antibody-modified microrings, we sequentially monitor primary CRP binding, secondary recognition bound by biotinylated antibody, and tertiary signal amplification using streptavidin-functionalized beads. This methodology is applied to in human serum plasma samples.
The human immune system consists of a large number T cells capable recognizing and responding to antigens derived from various sources. development peptide-major histocompatibility (p/MHC) tetrameric complexes has enabled the direct detection these antigen-specific cells. With goal increasing throughput multiplexing cell detection, protein microarrays spotted with defined p/MHC have been reported, but studies limited due inherent instability reproducibility arrays produced via conventional...
Optical resonator sensors are an emerging class of analytical technologies that use recirculating light confined within a microcavity to sensitively measure the surrounding environment. Bolstered by advances in microfabrication, these devices can be configured for wide variety chemical or biomolecular sensing applications. We begin with brief description optical sensor operation, followed discussions regarding design, including different geometries, choices material systems, methods...
Aniline-catalyzed hydrazone ligation between surface-immobilized hydrazines and aldehyde-modified antibodies is shown to be an efficient method for attaching protein capture agents model oxide-coated biosensor substrates. Silicon photonic microring resonators are used directly evaluate the efficiency of this surface bioconjugate reaction at various pHs in presence or absence aniline as a nucleophilic catalyst. It found that significantly increases net antibody loading surfaces functionalized...