A5060737425- Advanced MRI Techniques and Applications
- Advanced Neuroimaging Techniques and Applications
- RNA regulation and disease
- RNA Research and Splicing
- interferon and immune responses
- Neurogenesis and neuroplasticity mechanisms
- Microtubule and mitosis dynamics
- Anatomy and Medical Technology
- Surgical Simulation and Training
- Neuroscience and Neuropharmacology Research
- Ultrasound and Hyperthermia Applications
- Animal Behavior and Welfare Studies
- Muscle activation and electromyography studies
- Electrochemical Analysis and Applications
- RNA modifications and cancer
- Zebrafish Biomedical Research Applications
- Animal Vocal Communication and Behavior
- Electron Spin Resonance Studies
- bioluminescence and chemiluminescence research
- Nerve injury and regeneration
- Neuroinflammation and Neurodegeneration Mechanisms
- Soft Robotics and Applications
- Amino Acid Enzymes and Metabolism
- Hippo pathway signaling and YAP/TAZ
- Circadian rhythm and melatonin
Montreal Neurological Institute and Hospital
2020-2024
McGill University
2019-2024
Université de Montréal
2013
Myelin Basic Protein (MBP) is essential for both elaboration and maintenance of CNS myelin, its reduced accumulation results in hypomyelination. How different Mbp mRNA levels affect myelin dimensions across the lifespan how resident glial cells may respond to such changes are unknown. Here, investigate these questions, we used enhancer-edited mouse lines that accumulate ranging from 8% 160% wild type. In young mice, resulted corresponding decreases protein sheath thickness, confirming...
Because of their unique physicochemical properties, lanthanide-doped upconverting nanoparticles (Ln-UCNPs) have exceptional potential for biological applications. However, the use in systems is hampered by limited understanding bionano interactions. Our multidisciplinary study has generated these insights through in-depth and quantitative analyses. The Ln-UCNPs examined here are spherical, monodisperse, stable aqueous environments. We show that were associated with HeLa (cervical cancer)...
Abstract RNA polymerase III (Pol III)-related hypomyelinating leukodystrophy (POLR3-HLD), also known as 4H leukodystrophy, is a severe neurodegenerative disease characterized by the cardinal features of hypomyelination, hypodontia and hypogonadotropic hypogonadism. POLR3-HLD caused biallelic pathogenic variants in genes encoding Pol subunits. While approximately half all patients carry mutations POLR3B subunit B, there no vivo model based on mutation this subunit. Here, we determined impact...
Objectives/Hypothesis In animal studies of vocal fold scarring and treatment, imaging‐based evaluation is most often conducted by tissue slicing histological staining. Given variation in anatomy, injury type, severity, sacrifice timepoints, planar sections provide limited spatiotemporal details repair. Three‐dimensional (3D) virtual histology may additional contextual spatial information, enhancing objective interpretation. The study's aim was to evaluate the suitability magnetic resonance...
During locomotion, alternating activity of flexor and extensor muscles is largely regulated by a spinal neuronal network, the central pattern generator, which modulated peripheral supraspinal inputs. In absence these modulatory inputs, for example during fictive locomotion after spinalization curarization, spontaneous failures motor activation (deletions) in muscle can occur without perturbing rhythmic cycle structure antagonists on same side or contralateral side. This suggests that...
Melatonin is a neurohormone that modulates several physiological functions in mammals through the activation of melatonin receptor type 1 and 2 (MT1 MT2). The melatonergic system an emerging therapeutic target for new pharmacological interventions treatment sleep mood disorders; thus, imaging tools to further investigate its role brain are highly sought-after. We aimed develop selective radiotracers vivo both MT1 MT2 by positron emission tomography (PET). identified four previously reported...
Developmental and epileptic encephalopathies (DEEs) feature altered brain development, developmental delay seizures, with seizures exacerbating delay. Here we identify a cohort biallelic variants in DENND5A, encoding membrane trafficking protein, develop animal models phenotypes like the human syndrome. We demonstrate that DENND5A interacts Pals1/MUPP1, components of Crumbs apical polarity complex required for symmetrical division neural progenitor cells. Human induced pluripotent stem cells...
Non-invasive myelin water fraction (MWF) and g-ratio mapping using microstructural MRI have the potential to offer critical insights into brain microstructure our understanding of neuroplasticity neuroinflammation. By leveraging a unique panel variably hypomyelinating mouse strains, we validated high-resolution, model-free image reconstruction method for whole-brain MWF mapping. Further, by employing bipolar gradient echo sequence, achieved high spatial resolution robust across whole brain....
Abstract A growing body of research supports the hypothesis links between immune system activation and development Parkinson’s disease (PD). recent study revealed that repeated gastrointestinal infection with Citrobacter rodentium can lead to PD-like motor dysfunction in Pink1 knockout (KO) mice cell entry brain. With objective better understanding mechanisms leading attack brain this model, we evaluated such mild infections are sufficient increase blood barrier (BBB) permeability cause...
<h3>Objective:</h3> To develop the first model of RNA Polymerase III (Pol III)-Related Hypomyelinating Leukodystrophy (POLR3-HLD) based on a <i>POLR3B</i> mutation. <h3>Background:</h3> POLR3-HLD is devastating neurological disease characterized by severe diffuse hypomyelination and progressive functional decline leading to early death. It caused biallelic pathogenic variants in genes encoding Pol subunits. Patients are most commonly affected mutations <i>POLR3A</i> or <i>POLR3B</i>, but...
Multi-echo gradient echo (GRE) based, myelin water imaging (MWI) is subject to data quality constraints related signal noise ratio (SNR), B 0 field inhomogeneities and imperfections. These pose challenges for conventional model-free, non-negative least squares (NNLS) fitting. As an alternative, a three-pool parametric model can be applied, with nonlinear squares(NLLS) fitting process MWI data. The confers stability, but has the disadvantage of making prior assumptions. This study...
We investigated the application of blind source separation, robust principal component analysis (BSS-rPCA) for myelin water imaging in a panel mice exhibiting varied myelination profiles. BSS-rPCA exhibited sensitivity to as evidenced by region interest (ROI) several white matter tracts function hypomyelinating genotype. By combining with estimates neurite orientation dispersion and density (NODDI)-derived axon fraction (AWF) maps, we conclude that is sensitive variations content presence...
Abstract Developmental and epileptic encephalopathies (DEEs) are a heterogenous group of epilepsies in which altered brain development leads to developmental delay seizures, with the activity further negatively impacting neurodevelopment. Identifying underlying cause DEEs is essential for progress toward precision therapies. Here we describe individuals biallelic variants DENND5A determine that variant type correlated disease severity. We demonstrate interacts MUPP1 PALS1, components Crumbs...