The aim of the present study was to determine effects emodin, a natural compound with antioxidant properties, on oxidative stress and apoptosis induced by hypoxia/reoxygenation (H/R) in HK-2 human renal tubular cells. In cells subjected H/R, it observed that pre-treatment emodin lead an increase cellular viability reduction rate B-cell lymphoma 2 (Bcl-2)-associated X protein/Bcl-2 ratio. H/R alone caused significant levels reactive oxygen species malondialdehyde (P<0.05) decrease activities...

The cannabinoid receptor 1 (CB1) is famous as the target of Δ9-tetrahydrocannabinol (THC), which active ingredient marijuana. Suppression CB1 frequently suggested a drug or gene therapy for many conditions (e.g., obesity, Parkinson's disease). However, brain networks affected by remain elusive, and unanticipated psychological effects in clinical trial had dire consequences. To better understand whole suppression we performed vivo imaging on mice under complete knockout (cnr1-/-) also inverse...

Abstract Osteoarthritis (OA), a joint disease that is associated with inflammatory processes involved in destruction. Scutellarein (Scu), component of the medicinal herbs Scutellaria barbata D. Don and Erigeron breviscapus (vant) Hand Mass , has anti‐inflammatory effects. We explored role Scu development OA underlying mechanisms. CCK‐8 assays, Calcein‐AM/PI EdU staining were used to determine chondrocyte viability after exposure. Western blot, qPCR, as well ELISA utilized measure...

Attention-deficit/hyperactivity disorder (ADHD) is a common neurodevelopmental disease featuring executive control deficits as prominent neuropsychological trait. Executive functions are implicated in multiple sub-networks of the brain; however, few studies examine these whole ADHD. By combining resting-state functional MRI and graph-based approaches, we systematically investigated connectivity patterns among four control-related networks, including frontoparietal network (FPN),...

We have demonstrated recently that endothelin (ET) stimulates rat aortic smooth muscle cell proliferation through an extracellular signal–regulated kinase (ERK)–dependent mechanism. Approximately 70% of ET-dependent [ 3 H]-thymidine incorporation in these cells signals this system. In the present study, we show residual mitogenic activity requires intact p38 mitogen-activated protein (p38 MAPK) system and increased c-myc gene expression. ET ≈5-fold. MAPK inhibition with SB203580 or ERK/ERK...

We have shown in previous studies that high extracellular tonicity is associated with increased expression of the type A natriuretic peptide receptor (NPR-A) and reduced endothelial NO synthase (eNOS) gene cultured rat inner-medullary collecting duct cells. The vasoactive endothelin has been to be avidly expressed this nephron segment, subject osmotic regulation. asked whether might play a role control basal or osmotically regulated NPR-A eNOS these Although exogenous had little no effect on...

Self-assembly of planar supported lipid bilayers on top hydrophilic polymer brushes is a desirable alternative to solid and covalently tethered for applications like sensing transmembrane proteins which require large aqueous volume between membrane substrate. We present simple dip-and-rinse method produce poly(ethylene glycol) (PEG) with sparse positively charged hydrophobic tethers, using poly(l-lysine)-graft-poly(ethylene glycol)-quaternary ammonium compound copolymers. The interaction...

The aim of the study is to investigate diffusion characteristics Alzheimer's disease (AD) patients using an ultra-high b-values apparent coefficient (ADC_uh) and kurtosis imaging (DKI). A total 31 AD 20 healthy controls (HC) who underwent both MRI examination clinical assessment were included in this study. Diffusion weighted (DWI) was acquired with 14 range 0 5000 s/mm². Diffusivity analyzed selected regions, including amygdala (AMY), hippocampus (HIP), thalamus (THA), caudate...

The purpose of this study is to investigate the serum inflammatory factors in patients with high-altitude polycythemia (HAPC) and their correlation cognitive function. subjects were recruited placed into a HAPC group control group. Serum samples collected, (interleukin-1beta [IL-1β], monocyte chemoattractant protein-1 [MCP-1], tumor necrosis factor-alpha [TNF-α]) measured using ELISA kits. mini-mental State Examination (MMSE) was used assess According MMSE scores, further divided normal...

Obesity is associated with insulin resistance (IR) and hepatosteatosis. Understanding the link between IR hepatosteatosis could be relevant to chronic clinical outcomes. The objective of this study was quantitatively assess lipid deposition (fractional mass, fLM) composition (fraction polyunsaturated lipids, fPUL mean chain length, MCL) in livers ob/ob mice, a genetic model obesity mild diabetes, ob/+ heterozygous control animals noninvasive manner using 1 H‐MRS at 9.4T. For accurate...

Background Conventional diffusion‐weighted imaging (DWI) with high b‐values may improve lesion conspicuity, but a low signal intensity and thus signal‐to‐noise ratio (SNR). The voxelwise computed DWI (vcDWI) generate high‐quality images strong background. Purpose To evaluate the feasibility diagnostic performance of vcDWI. Study Type Retrospective. Population In all, 67 patients 72 lesions, 33 malignant 39 benign. Field Strength/Sequence 3T, including T 2 /T 1 , two b‐values, dynamic...

Lynch syndrome is the most common hereditary colorectal cancer syndrome, and adenoma one of important premalignant lesions to in syndrome. The first objective this study was calculate detection rate polyps by using mismatch repair immunohistochemistry as initial screening strategy. second optimize strategies for associated with integrating polyp and/or patient characteristics such size, location, dysplasia, age onset family history cancer. From June 2014 May 2016, performed proteins (MLH1,...

Purpose: To demonstrate the potential benefits of T2* relaxation time intervertebral discs (IVDs) regarding detection and grading degenerative disc disease using 3.0-T magnetic resonance imaging (MRI) in a clinical setting. Materials Methods: Cervical sagittal T2-weighted, MRI was performed at 61 subjects, covering C2–3 to C6–7. All were morphologically assessed based on Pfirrmann grade, regions interests (ROIs) drawn over mapping. Receiver operating characteristic (ROC) analysis among...

Purpose: This study aimed to establish the prevalence of pulmonary embolism (PE) in chronic obstructive disease (COPD) patients with secondary polycythemia (SP) and explore risk factors for PE COPD SP. Patients Methods: We analyzed among SP who were hospitalized at Qinghai Provincial People's Hospital between January 2015 December 2020. From 2021 2024, we enrolled into three groups (COPD+SP+PE, COPD+SP, control) performed laboratory measurements, biomarkers, echocardiography, function tests....

A central step in the initiation of chromosomal DNA replication eukaryotes is assembly pre-replicative complex (pre-RC) at late M and early G1 phase cell cycles. Since 1973, four proteins or protein complexes, including division control 6 (Cdc6)/Cdc18, minichromosome maintenance complex, origin recognition (ORC), Cdt1, are known components pre-RC. Previously, we reported that a non-ORC binds to essential element Δ9 Schizosaccharomyces pombe DNA-replication ARS3001. In this study, identified...

Background The effectiveness of quantitative MRI and clinical information in the risk stratification prostate cancer (PCa) patients was evaluated separately previous research; however, differentiation power combining has yet to be investigated. Purpose To investigate histogram analysis apparent diffusion coefficient (ADC) tumor volume (tDv) with for low‐grade (Gleason score [GS] ≤6) high‐grade (GS ≥7) PCa. Study Type Retrospective. Population Fifty‐nine PCa who underwent preoperative...

Objective. Genetic variation, especially polymorphism of the dopamine D4 receptor gene (DRD4), has been linked to deficits in self-regulation and executive functions attention deficit hyperactivity disorder (ADHD), is related structural functional integrity default mode network (DMN), control (ECN) sensorimotor (SMN). The aim this study was explore effects 2-repeat allele DRD4 on brain connectivity behaviors children with ADHD. Methods. Using independent component analysis dimension...

Insulin resistance is a central feature of type II diabetes and associated with alterations in skeletal muscle lipid metabolism, which manifest themselves, part, increased intramyocellular (IMCL) accumulation. The objective this study was to assess noninvasively the levels IMCL longitudinally tibialis anterior Lep ob /Lep (ob/ob) mice, genetic model obesity mild diabetes, /+ (ob/ + ) heterozygous control animals, using 1 H MRS at 9.4 T. use cryogenic surface coil transceiver leads...