Ultrasound mediated drug delivery, a promising therapeutic modality, has evolved remarkably over the past three decades. Initially designed to enhance contrast in ultrasound imaging, microbubbles have emerged as main vector for offering targeted therapy with minimized side effects. This review addresses historical progression of this technology, emphasizing pivotal role play augmenting extravasation and delivery. We explore complex mechanisms behind from stable inertial cavitation diverse...

The RNA interference-mediated gene silencing approach is promising for therapies based on the targeted inhibition of disease-relevant genes. Electropermeabilization one nonviral methods successfully used to transfer siRNA into living cells in vitro and vivo. Although this effective field by interference, very little known about basic processes supporting transfer. In study, we investigated, direct visualization at single-cell level, delivery Alexa Fluor 546-labeled murine melanoma stably...

Doxorubicin is a potent chemotherapeutic whose severe side effects limit its application. Drug-targeted delivery with noninvasive techniques required to increase the drug concentration locally and reduce systemic effects. Microbubble-assisted ultrasound has become promising strategy for local delivery. The aim of this study evaluate applicability effectiveness administration doxorubicin combined microbubble-assisted in human U-87MG glioblastoma MDA-MB-231 breast cancer cells. In present...

Electroporation is a physical method used to transfer molecules into cells and tissues. Clinical applications have been developed for antitumor drug delivery. trials of gene electrotransfer are under investigation. However, knowledge about how DNA enters not complete. By contrast small that direct access the cytoplasm, forms long lived complex with plasma membrane transferred cytoplasm considerable delay.To increase our understanding key step DNA/membrane formation, we investigated...

Targeted drug delivery under image guidance is gaining more interest in the drug-delivery field. The use of microbubbles as contrast agents diagnostic ultrasound provides new opportunities noninvasive image-guided delivery. In present study, imaging and therapeutic properties novel doxorubicin liposome-loaded are evaluated. results showed that at scanning settings (1.7 MHz mechanical index 0.2), these scatter sufficient signal for nonlinear can thus be imaged real time tracked vivo. vitro...

Electrotransfer of molecules is a well established technique which finds extensive use for gene transfer and holds great promise anticancer treatment. Despite its widespread application, the mechanisms governing entry DNA into cell intracellular trafficking are not yet known. The aim this study to unravel role actin cytoskeleton during electrotransfer in cells. We performed single-cell level approaches observe organization Chinese hamster ovary (CHO) In addition, we experiments at...

Treatment of recurrent or unresectable cutaneous and subcutaneaous tumors continues to be a major therapeutic challenge. Electrochemotherapy (ECT) is palliative treatment subcutaneous tumor nodules for which standard treatments (e.g. radiotherapy, chemotherapy, surgery) have failed proved insufficient. ECT combines the electropulsation cells (by local application electric pulses) administration antineoplastic drugs such as cisplatin bleomycin (either intravenous intratumoral). The...

Irinotecan is a powerful anticancer drug with severe systemic side effects that limit its clinical application. Drug-targeted delivery noninvasive methods required to enhance the concentration locally and reduce these undesirable events. Microbubble-assisted ultrasound has become promising method for targeted delivery. The aim of this study evaluate therapeutic effectiveness in vitro vivo irinotecan based on combination microbubbles. In present study, results showed treatment...

Sonoporation is a physical method that has been successfully used to deliver drugs into living cells both in vitro and vivo for experimental therapeutic purposes. Despite numerous studies on this topic, often reporting successful outcomes, very little known about the mechanisms involved hypothesized membrane permeabilization processes. In study, electron microscopy was investigate ultra-structural modifications of cell membranes, induced by sonoporation. Here, we demonstrate sonoporation...

To date, no treatment has been developed for targeted delivery to the inner ear (IE). Sonoporation, a promising drug method, increases permeability of round window membranes (RWMs), enhancing diffusion IE. A dedicated ultrasound protocol is essential treat IE pathologies in combination with sonoporation. In situ acoustic pressure (AP) measurements cannot be used RWM sonoporation because heterogeneous anatomy temporal bone. This study aimed model propagation ensure adequate AP The impact...

Background: The blood–brain barrier (BBB) is a selective and semi-permeable essential for protecting the brain’s parenchyma against pathogens toxic molecules present in bloodstream. It consists of monolayer brain capillary endothelial cells, pericytes, astrocytic end-feet, neurons. tight junctions between cells prevent paracellular transport, further reinforcing its selectivity. However, this high level selectivity represents significant challenge delivery therapeutic to...

Gene electrotransfer is a nonviral method used for DNA delivery into cells. Several steps are involved. One of them the interaction with cell membrane, which crucial before can enter cell. We analysed level DNA-membrane in relation to efficiency and importance electrophoretic accumulation at membrane. Systematic comparison long-duration, short-duration combinations electropermeabilizing short (high-voltage; HV) long (low-voltage; LV) pulses were performed. The effect Mg(2+) ion...

This technical note investigates the involvement of microbubble attenuation and destruction in sonoporation mechanisms. First, we evaluate efficiency using Vevo Micromarker, a comparison is made with BR14 SonoVue microbubbles. Then, acoustical properties microbubbles are measured to gain insight into mechanisms green fluorescent protein as marker. Using glioblastoma cells, an unprecedented transfection rate 70% reached corresponding 1.5-fold increase compared achieved other Moreover, were...

One of the applications electroporation/electropulsation in biomedicine is gene electrotransfer, wider use which hindered by low transfection efficiency vivo compared with viral vectors. The aim our study was to determine whether modulation extracellular matrix solid tumors, using collagenase and hyaluronidase, could increase electrotransfer histologically different subcutaneous tumors mice. Tumors were treated enzymes before plasmid DNA encoding either green fluorescent protein or...

This preclinical study aimed to evaluate placental oxygenation in pregnant rats by real-time photoacoustic (PA) imaging on different days of gestation and specify variations oxygen saturation under conditions maternal hypoxia hyperoxygenation.Placentas fifteen Sprague-Dawley were examined 14, 17, 20 pregnancy with a PA system coupled high-resolution ultrasound imaging. Pregnant successively exposed hyperoxygenated hypoxic changing the concentration inhaled gas. Tissue was quantitatively...