A5018486143- Neurogenesis and neuroplasticity mechanisms
- Axon Guidance and Neuronal Signaling
- Nerve injury and regeneration
- Cellular transport and secretion
- Microtubule and mitosis dynamics
- Neuroscience and Neuropharmacology Research
- Congenital heart defects research
- Developmental Biology and Gene Regulation
- Lipid Membrane Structure and Behavior
- Signaling Pathways in Disease
- Hippo pathway signaling and YAP/TAZ
- Marine Sponges and Natural Products
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Mitochondrial Function and Pathology
- Single-cell and spatial transcriptomics
Universitat de Barcelona
2018-2023
University of California, San Diego
2023
Biomedical Research Networking Center on Neurodegenerative Diseases
2018-2021
Helmholtz Association of German Research Centres
2020-2021
Instituto de Salud Carlos III
2018-2020
Centro de Investigación Biomédica en Red
2019
Axonal growth during normal development and axonal regeneration rely on the action of many receptor signaling systems complexes, most them located in specialized raft membrane microdomains with a precise lipid composition. Cholesterol is component rafts, integrity these structures depends concentrations present this compound. Here we explored effect cholesterol depletion both developing neurons regenerating axons. First, show that vitro from central peripheral nervous increases size cones,...
Despite substantial progress in understanding the biology of axon regeneration CNS, our ability to promote clinically important corticospinal tract (CST) after spinal cord injury remains limited. To understand regenerative heterogeneity, we conducted patch-based single-cell RNA sequencing on rare regenerating CST neurons at high depth following PTEN and SOCS3 deletion. Supervised classification with Garnett gave rise a Regeneration Classifier, which can be broadly applied predict potential...
The mature cerebellum controls motor skill precision and participates in other sophisticated brain functions that include learning, cognition, speech. Different types of GABAergic glutamatergic cerebellar neurons originate temporal order from two progenitor niches, the ventricular zone rhombic lip, which express transcription factors Ptf1a Atoh1, respectively. However, molecular machinery required to specify distinct neuronal emanating these zones is still unclear. Here, we uncover factor...
Axonal growth and guidance rely on correct cone responses to cues. Unlike the signaling cascades that link axonal cytoskeletal dynamics, little is known about crosstalk mechanisms between membrane dynamics turnover. Recent studies indicate whereas attraction requires exocytosis, chemorepulsion relies endocytosis. Indeed, our own have shown Netrin-1/Deleted in Colorectal Cancer (DCC) triggers exocytosis through SNARE Syntaxin-1 (STX1). However, limited vivo evidence available role of proteins...
Abstract During brain development, Uncoordinated locomotion 5 (UNC5) receptors control axonal extension through their sensing of the guidance molecule Netrin-1. The correct positioning into cholesterol-enriched membrane raft microdomains is crucial for efficient transduction recognized signals. However, whether such are required appropriate mediated by UNC5 remains unknown. Here, we combine use confocal microscopy, live-cell FRAP analysis and single-particle tracking PALM to characterize...
Neuronal cell adhesion molecule 2 (NCAM2) is a membrane protein with an important role in the morphological development of neurons. In cortex and hippocampus, NCAM2 essential for proper neuronal differentiation, dendritic axonal outgrowth synapse formation. However, little known about functional mechanisms its interactive partners during brain development. Here we used mass spectrometry to study molecular interactome second postnatal week mouse cerebral cortex. We found that interacts...
Nystatin is a pharmacological agent commonly used for the treatment of oral, mucosal and cutaneous fungal infections. has also been extensively applied to study cellular function cholesterol-enriched structures because its ability bind extract cholesterol from mammalian membranes. In neurons, level tightly regulated, being essential synapse dendrite formation, axonal guidance. However, action on axon regeneration poorly evaluated. Here, we examine effect primary cultures hippocampal showing...
Introduction Brain connectivity requires correct axonal guidance to drive axons their appropriate targets. This process is orchestrated by cues that exert attraction or repulsion developing axons. However, the intricacies of cellular machinery responsible for response growth cones are just being unveiled. Netrin-1 a bifunctional molecule involved in axon pathfinding and cell migration induces during postnatal cerebellar development. mediated UNC5 homolog receptors located on external granule...
Abstract The corticospinal tract (CST) is clinically important for the recovery of motor functions after spinal cord injury. Despite substantial progress in understanding biology axon regeneration central nervous system (CNS), our ability to promote CST remains limited. Even with molecular interventions, only a small proportion axons regenerate1. Here we investigate this heterogeneity regenerative neurons following PTEN and SOCS3 deletion patch-based single cell RNA sequencing...
Abstract The mature cerebellum controls motor skill precision and participates in other sophisticated brain functions that include learning, cognition speech. Different types of GABAergic glutamatergic cerebellar neurons originate temporal order from two progenitor niches, the ventricular zone rhombic lip, which express transcription factors Ptf1a Atoh1, respectively. However, molecular machinery required to specify distinct neuronal emanating these zones is still unclear. Here, we uncover...
Abstract Brain connectivity requires correct axonal guidance to drive axons their appropriate targets. This process is orchestrated by cues that exert attraction or repulsion developing axons. However, the intricacies of cellular machinery responsible for response growth cones are just being unveiled. Netrin-1 a bifunctional molecule involved in axon pathfinding and cell migration induces during postnatal cerebellar development. mediated Uncoordinated locomotion 5 (UNC5) receptors located on...