A5087167031- Immune cells in cancer
- Glioma Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
- Single-cell and spatial transcriptomics
- Ferroptosis and cancer prognosis
- CAR-T cell therapy research
- Extracellular vesicles in disease
- Brain Metastases and Treatment
- Birth, Development, and Health
- Diabetes and associated disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Phagocytosis and Immune Regulation
- Gestational Diabetes Research and Management
- Prenatal Substance Exposure Effects
- Adipokines, Inflammation, and Metabolic Diseases
- Diet and metabolism studies
- Metabolism, Diabetes, and Cancer
- Lymphoma Diagnosis and Treatment
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
The Netherlands Cancer Institute
2020-2025
Dutch Cancer Society
2025
Oncode Institute
2020-2024
Brigham and Women's Hospital
2020
Harvard University
2020
Abstract Single-cell technologies have enabled the characterization of tumour microenvironment at unprecedented depth and revealed vast cellular diversity among cells their niche. Anti-tumour immunity relies on cell–cell relationships within 1,2 , yet many single-cell studies lack spatial context rely dissociated tissues 3 . Here we applied imaging mass cytometry to characterize immunological landscape 139 high-grade glioma 46 brain metastasis tumours from patients. analysis more than 1.1...
Glioblastomas are aggressive primary brain tumors with an inherent resistance to T cell-centric immunotherapy due their low mutational burden and immunosuppressive tumor microenvironment. Here we report that fractionated radiotherapy of preclinical glioblastoma models induce a tenfold increase in cell content. Orthogonally, spatial imaging mass cytometry shows enrichment human recurrent compared matched glioblastoma. In glioblastoma-bearing mice, α-PD-1 treatment applied at the peak...
Tumors growing in metabolically challenged environments, such as glioblastoma the brain, are particularly reliant on crosstalk with their tumor microenvironment (TME) to satisfy high energetic needs. To study intricacies of this metabolic interplay, we interrogated heterogeneity TME using single-cell and multi-omics analyses identified rewired tumor-associated macrophage (TAM) subpopulations pro-tumorigenic properties. These TAM subsets, termed lipid-laden macrophages (LLMs) reflect...
Abstract Myeloid cells are abundant and plastic immune cell subsets in the liver, to which pro-tumorigenic, inflammatory immunosuppressive roles have been assigned course of tumorigenesis. Yet several aspects underlying their dynamic alterations hepatocellular carcinoma (HCC) progression remain elusive, including impact distinct genetic mutations shaping a cancer-permissive tumor microenvironment (TME). Here, newly generated, clinically-relevant somatic female HCC mouse models, we identify...
Abstract Background Glioblastoma is a treatment-resistant brain cancer. Its hierarchical cellular nature and its tumor microenvironment (TME) before, during, after treatments remain unresolved. Methods Here, we used single-cell RNA sequencing to analyze new recurrent glioblastoma the nearby subventricular zone (SVZ). Results We found 4 neural lineages are present in with an enrichment of cancer mesenchymal lineage, immune cells, reactive astrocytes early recurrences. Cancer were...
Prenatal alcohol exposure (PAE) affects at least 10% of newborns globally and leads to the development fetal spectrum disorders (FASDs). Despite its high incidence, there is no consensus on implications PAE metabolic disease risk in adults. Here, we describe a cohort adults with FASDs that had an increased incidence abnormalities, including type 2 diabetes, low HDL, triglycerides, female-specific overweight obesity. Using zebrafish model for PAE, performed population studies elucidate seen...
Abstract Myeloid cells are abundant and plastic immune cell subsets in the liver, to which pro-tumorigenic, inflammatory immunosuppressive roles have been assigned course of tumorigenesis. Yet several aspects underlying their dynamic alterations hepatocellular carcinoma (HCC) progression remain elusive, including impact distinct genetic mutations shaping a cancer-permissive tumor microenvironment (TME). Here, we generated somatic HCC mouse models bearing clinically-relevant oncogenic driver...