A5101663164- Receptor Mechanisms and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Analytical Chemistry and Chromatography
- Advanced Biosensing Techniques and Applications
- Protein Interaction Studies and Fluorescence Analysis
- Pharmacological Effects of Natural Compounds
- Bone Metabolism and Diseases
- Pharmacological Effects and Assays
- Catalytic C–H Functionalization Methods
- Chemical Synthesis and Analysis
- Traditional Chinese Medicine Analysis
- Sulfur-Based Synthesis Techniques
- Macrophage Migration Inhibitory Factor
- Catalytic Cross-Coupling Reactions
- Photonic and Optical Devices
- Estrogen and related hormone effects
- Primate Behavior and Ecology
- Neuroscience and Neural Engineering
- Biotin and Related Studies
- Lipid Membrane Structure and Behavior
- Computational Drug Discovery Methods
- Electrochemical sensors and biosensors
- Cardiac electrophysiology and arrhythmias
- Analytical Chemistry and Sensors
- Trace Elements in Health
Northwest University
2016-2025
Ministry of Education of the People's Republic of China
2015-2025
Chinese Academy of Fishery Sciences
2025
Harbin Medical University
2012-2024
Northwest University
2015-2024
Northwest Institute of Nuclear Technology
2024
Civitan International
2024
University of Alabama at Birmingham
2024
Jiangnan University
2020-2024
State Key Laboratory of Food Science and Technology
2024
Members of the hyperpolarization-activated cation (HCN) channel family generate HCN currents (Ih) that are directly regulated by cAMP and contribute to pacemaking activity in heart brain. The four different isoforms show distinct biophysical properties. In cell-free patches from Xenopus oocytes, steady-state activation curve HCN2 channels is 20 mV more hyperpolarized compared with HCN1. Whereas binding a COOH-terminal cyclic nucleotide domain (CNBD) markedly shifts 17 positive potentials,...
An approach is established for the specific immobilization of GPCRs from cell lysates that circumvents labor intensive purification procedures and minimize loss activity.
Protein immobilization is particularly significant in proteomics, interactomics, and vitro drug screening. It an essential primary step for numerous biological techniques that rely on immobilized proteins with controlled orientation, high conformational stability, activity (CHH). These have challenged the current strategy demanded increasing efforts efficient method to meet CHH a single step. Herein, we proposed covalent inhibitor-based, one-step G protein-coupled receptor (GPCR) inspired by...
Paeoniflorin (Pae), a water-soluble monoterpene glucoside, has high potential clinical value in autoimmune and inflammatory diseases. However, the extremely low oral bioavailability of Pae (approximately 3%-4%) limits its formulation development application. This study aimed to develop micelles using glycyrrhizic acid (GL) as carrier improve absorption Pae.Pae-loaded GL were prepared by ultrasonic dispersion method was optimized single-factor tests. Characterizations Pae-loaded including...
The presence of intermuscular bones severely affects the edibility and value-added processing crucian carp (Carassius auratus), becoming a constraint to high-quality development its industry. Our previous study identified bmp6 as key osteogenic regulator successfully developed new strain lacking (WUCI) using CRISPR/Cas9 technology. To accelerate industrialization, we comprehensively assessed WUCI’s growth performance, hematological parameters, antioxidant capacity, innate immunity, disease...
Over the past two decades, fluoride effects on osteoclasts have been evaluated; however, its molecular mechanisms remain unclear. In this study, we investigated effect of osteoclast formation, function, and regulation using formed from mice bone marrow macrophages treated with receptor activator NF‐κB ligand macrophage colony‐stimulating factor. Our data showed that levels ≤ 8 mg/L had no formation; it significantly reduced resorption at 0.5 mg/L. Fluoride activity occurred through...
Although a comparatively robust method, immobilized protein-based techniques have displayed limited precision and inconsistent results due to lack of strategy for the accurate selection drug adsorption models on protein surface. We generated data three drugs beta-2-adrenoceptor (β2-AR) by frontal affinity chromatography–mass spectrometry (FAC-MS) site-specific competitive FAC-MS. Using energy distribution (AED) calculations, we achieved best binding salbutamol, terbutaline, pseudoephedrine...
Postmenopausal osteoporosis (POP) is quite prevalent and many new drugs are under development to obtain better therapeutic outcomes. Oleanolic acid (OA) has been reported prevent bone loss in ovariectomized (OVX) rats by stimulating osteoblastogenesis. One previous study demonstrated that acetate of OA suppressed lipopolysaccharides (LPS)-induced mice. However, the role receptor activator nuclear factor kappa-B ligand (RANKL)-mediated osteoclastogenesis still not elucidated. Here we show...
Natural products have failed to meet the urgent need for drug discovery in recent decades due limited resources, necessitating new strategies re-establishing key role of natural hit screening. This work introduced DNA-encoding techniques into synthesis phenolic acid-focused libraries containing 32 000 diverse compounds. Online selection library using immobilized angiotensin II type I receptor (AT
Dietary oat β-glucan is metabolized human gut Bacteroides via glycoside hydrolases and further affects health.
A high level of plasma homocysteine (Hcy) increases the risk for neurodegenerative diseases. One such disorder is Alzheimer's disease, which involves marked neuronal apoptosis unknown etiology. This study shows that Hcy inhibits expression 14-3-3ε and activates calcineurin in rat hippocampal neurons a dose-dependent manner. Calcineurin-mediated Bad dephosphorylation, blocked by inhibition Ca2+ chelation, causes mitochondrial translocation apoptosis; this step apoptotic pathway...