A5030963172- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Diabetes and associated disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Pancreatitis Pathology and Treatment
- Extracellular vesicles in disease
- Advanced Proteomics Techniques and Applications
- Bioinformatics and Genomic Networks
- S100 Proteins and Annexins
- Olfactory and Sensory Function Studies
- Epigenetics and DNA Methylation
- Protease and Inhibitor Mechanisms
- Congenital Diaphragmatic Hernia Studies
- Tryptophan and brain disorders
- Marine Biology and Environmental Chemistry
- Nanoplatforms for cancer theranostics
- Environmental Toxicology and Ecotoxicology
- Peroxisome Proliferator-Activated Receptors
- Neonatal Respiratory Health Research
- Endoplasmic Reticulum Stress and Disease
- Marine Toxins and Detection Methods
- Amyotrophic Lateral Sclerosis Research
- Phagocytosis and Immune Regulation
- Glycosylation and Glycoproteins Research
University of Geneva
2015-2024
Swiss Centre for Applied Human Toxicology
2019-2022
Geneva College
2014
The success of malignant tumors is conditioned by the intercellular communication between tumor cells and their microenvironment, with extracellular vesicles (EVs) acting as main mediators. While value 3D conditions to study well established, impact cellular architecture on EV content function not investigated yet. Here, a recently developed cell culture microwell array adapted for production comprehensive comparative analysis biochemical features, RNA proteomic profiles EVs secreted 2D vs...
Abstract Malaria-causing parasites of the Plasmodium genus undergo multiple developmental phases in human and mosquito hosts, regulated by various post-translational modifications. While ubiquitination multi-component E3 ligases is key to regulate a wide range cellular processes eukaryotes, little known about its role . Here we show that berghei expresses conserved SKP1/Cullin1/FBXO1 (SCF FBXO1 ) complex showing tightly expression localisation across stages. It cell division for nuclear...
Studies on the pathophysiology of type 2 diabetes mellitus (T2DM) have linked accumulation lipid metabolites to development beta-cell dysfunction and impaired insulin secretion. In most in vitro models T2DM, rodent islets or lines are used typically focus is specific cellular pathways organs. Our aim was to, firstly, develop a combined lipidomics proteomics approach for lipotoxicity isolated human and, secondly, investigate if could delineate novel and/ confirm reported mechanisms...
Astrogliosis has been abundantly studied in rodents but relatively poorly human cells due to limited access the brain. Astrocytes play important roles cerebral energy metabolism, and are also key players neuroinflammation. Astroglial metabolic inflammatory changes as a function of age have reported, leading hypothesis that mitochondrial metabolism responses interconnected supporting functional switch astrocytes from neurotrophic neurotoxic. This study aimed explore occurring during their...
Objective: We have previously shown the existence of a muscle-pancreas intercommunication axis in which CX3CL1 (fractalkine), CX3C chemokine produced by skeletal muscle cells, could be implicated.It has recently been that fractalkine system modulates murine βcell function.However, impact on human islet cells especially regarding protective role against cytokine-induced apoptosis remains to investigated.Methods: Gene expression was determined using RNA sequencing islets, sorted β -and non...
Morphine, a commonly used antinociceptive drug in hospitals, is known to cross the blood-brain barrier (BBB) by first passing through brain endothelial cells. Despite its pain-relieving effect, morphine also has detrimental effects, such as potential induction of redox imbalance brain. However, there still insufficient evidence these effects on brain, particularly cells and extracellular vesicles that they naturally release. Indeed, (EVs) are nanosized bioparticles produced almost all cell...
Abstract Morphine is one of the most potent opioid analgesic used for pain treatment. action in central nervous system requires crossing blood–brain barrier. Due to controversial relationship between morphine and oxidative stress, potential pro- or antioxidant effects barrier important be understood, as stress could cause its disruption predispose neurodegenerative diseases. However, investigation scarce human brain endothelial cells. Therefore, present study evaluated impact exposure at...
Toxicology studies can take advantage of omics approaches to better understand the phenomena underlying phenotypic alterations induced by different types exposure certain toxicants. Nevertheless, in order analyse data generated from multifactorial studies, dedicated analysis tools are needed. In this work, we propose a new workflow comprising both factor deconvolution and integration multiple analytical platforms. As case study, 3D neural cell cultures were exposed trimethyltin (TMT)...
Type 2 diabetes is a worldwide disease reaching epidemic dimensions. The rapid progression of the urgently calls for both broader and deeper understanding its pathophysiology. In line with this statement, Human Diabetes Proteome Project (HDPP) was officially launched at 11th HUPO meeting in Boston, 2012. A special session dedicated to new initiative, gathering experts main topics related associated complications. Key issues were debated focus on how deranged circulating glucose free fatty...
Insulin secretory granules are β-cell vesicles dedicated to insulin processing, storage, and release. The secretion of granule content in response an acute increase glucose concentration is a highly regulated process allowing normal glycemic homeostasis. Type 2 diabetes metabolic disease characterized by chronic hyperglycemia. consequent prolonged exposure known exert deleterious effects on the function various organs, notably impairment pancreatic β-cells induction apoptosis. It has also...
Osteogenesis imperfecta (OI) is a heritable disorder that mainly affects the skeleton. The inheritance mostly autosomal dominant and associated to mutations in one of two genes, COL1A1 COL1A2, encoding for type I collagen α chains. According more than 1500 described mutation sites outcome spanning from very mild cases perinatal-lethality, OI characterized by wide genotype/phenotype heterogeneity. In order identify common affected molecular-pathways disease biomarkers probands with different...
Abstract Despite Paraquat (PQ) being banned in several countries, it is still one of the most commonly used herbicides agriculture. This compound known to induce damaging effects on human and animal brain cells by generating Reactive Oxygen Species (ROS). However, there few evidence PQ effect Human Brain Microvascular Endothelial Cells (HBMECs), major component Blood–Brain Barrier (BBB). The present study aimed at unraveling biological mechanisms associated exposure 1, 10 100 µM for 24 h...
Pancreatic β-cells are responsible for insulin secretion that regulates blood glucose homeostasis. In the development of type II diabetes, a progressive impairment by pancreatic occurs called β-cell dysfunction or failure. Chronic hyperglycemia has been shown being involved in dysfunction, phenomenon known as glucotoxicity. The molecular mechanisms underlying induced glucotoxicity still not fully understood. this work, quantitative proteomics was employed to identify early key players For...
In obese children with high circulating concentrations of free fatty acid palmitate, we have observed that insulin levels at fasting and in response to a glucose challenge were several times higher than low the as well lean controls. Declining even insufficient adolescents acid. isolated human islets exposed palmitate hypersecretion after 2 days exposure. contrast, secretion from was reduced 7 culture presence This study aims identifying islet-related biological events potentially linked...
Diabetes is an increasing worldwide problem leading to major associated health issues and increased care costs.In 2012, 9.3% of the American population was affected by diabetes, according Association, with 1.7 million new cases since during year (www.diabetes.org).Proteome initiatives can provide a deeper understanding biology this disease help develop more effective treatments.The collaborative effort Human Proteome Project (HDPP) brings together wide variety complementary resources...