A5050949582- Gut microbiota and health
- Tryptophan and brain disorders
- Diet and metabolism studies
- Alzheimer's disease research and treatments
- Stress Responses and Cortisol
- Clostridium difficile and Clostridium perfringens research
- Birth, Development, and Health
- Medicinal Plants and Neuroprotection
- Colorectal Cancer Screening and Detection
- Dental Health and Care Utilization
- Anesthesia and Neurotoxicity Research
- Biotin and Related Studies
- Genomics and Phylogenetic Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Adipose Tissue and Metabolism
- Probiotics and Fermented Foods
- Health, psychology, and well-being
Istituti di Ricovero e Cura a Carattere Scientifico
2017-2021
Centro San Giovanni di Dio Fatebenefratelli
2016-2021
Amplicon high-throughput sequencing of 16S ribosomal RNA (rRNA) gene is currently the most widely used technique to investigate complex gut microbial communities. Microbial identification might be influenced by several factors, including choice bioinformatic pipelines, making comparisons across studies difficult. Here, we compared four commonly pipelines (QIIME2, Bioconductor, UPARSE and mothur) run on two operating systems (OS) (Linux Mac), evaluate impact pipeline OS taxonomic...
Background: No study investigated the association between stress exposure in different stages of life and metabolic dysfunction. Aim: We explore several biomarkers related to glucose metabolism (insulin, c-peptide, GIP, GLP-1, glucagon) a group 72 healthy individuals. Method: used Childhood Experience Care Abuse-Questionnaire (CECA-Q) modified version Life Events Scale define stress, according four categories: no childhood trauma (CT) nor stressful events (SLEs) (46%), only CT (25%), SLEs...
Increasing evidence shows that the microbiome hosted by human gut may be involved in pathogenesis of several neurodegenerative disorders, including Alzheimer's Disease (AD). This study aimed at evaluating whether AD patients are characterized an altered fecal microbiota composition. Fecal samples were collected from 24 cognitively intact older persons (HC) and 30 amyloid positive patients. DNA was extracted frozen stools using QIAamp Fast stool kit after mechanical disruption (bead-beating)....
The gut-brain axis is attracting increasing interest in the Alzheimer's and dementia field. Amplicon high-throughput sequencing of 16S ribosomal RNA (rRNA) gene most used technique to characterize complex gut microbial communities. Several factors, including bioinformatic pipelines applied, may influence results on composition flora, preventing comparisons across studies. This study aims at evaluating effect taxonomic classification bacteria stools patients. Fecal samples were collected from...