A5047191453- Indoor Air Quality and Microbial Exposure
- Air Quality and Health Impacts
- Occupational exposure and asthma
- Mycotoxins in Agriculture and Food
- Odor and Emission Control Technologies
- Health, Environment, Cognitive Aging
- Air Quality Monitoring and Forecasting
- Antifungal resistance and susceptibility
- Fungal Plant Pathogen Control
- Plant Pathogens and Fungal Diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Occupational and environmental lung diseases
- Allergic Rhinitis and Sensitization
- Plant and fungal interactions
- Legionella and Acanthamoeba research
- Effects of Vibration on Health
- Transgenic Plants and Applications
- MicroRNA in disease regulation
- Animal testing and alternatives
- Cooperative Studies and Economics
- Infection Control and Ventilation
- Environmental DNA in Biodiversity Studies
- Plant-Microbe Interactions and Immunity
- Food Allergy and Anaphylaxis Research
- Delphi Technique in Research
National Institute of Occupational Health
2016-2025
Norwegian Veterinary Institute
2023
Government of India
2022
National Institute of Occupational Health
2022
Massey University
2018-2019
Victoria University of Wellington
2018
University of Oslo
2001
As a complex system governing and interconnecting numerous functions within the human body, immune is unsurprisingly susceptible to impact of toxic chemicals. Toxicants can influence through multitude mechanisms, resulting in immunosuppression, hypersensitivity, increased risk autoimmune diseases cancer development. At present, regulatory assessment immunotoxicity chemicals relies heavily on rodent models limited number Organisation for Economic Co-operation Development (OECD) test...
Introduction Waste management represents an occupational setting where fungi are significant contaminants. This study aimed to assess the exposure of waste workers mycotoxins through a human biomonitoring study. Methods A total 33 and 19 controls provided spot urine samples determine 10 mycotoxins’ urinary biomarkers using liquid chromatography coupled with mass spectrometry. Risk characterization was performed hazard quotient margin assessments. Results The results indicated that were...
Introduction Shellfish processing workers are highly susceptible to respiratory illnesses such as allergies and asthma. This study examined symptoms biomarkers of allergy asthma in Norwegian shrimp plant evaluated allergenic irritant protein exposures the workplace. Material methods The included 35 21 controls. Respiratory were assessed via questionnaire; blood samples analysed for specific IgE levels. Air levels composition. Results Shrimp had four five times higher odds reporting acute...
Abstract Exposure to hazardous microorganisms during waste handling is a potential health concern. Molecular biological techniques provide means of profiling the microbial community at high taxonomic resolution, allow identification critical human pathogens on species level and thereby aid risk assessment work tasks. The present study used high-throughput sequencing characterise microbiome in personal full-shift air samples collected contemporary sorting plants (WSPs) identified large...
M cells in follicle-associated epithelium of Peyer's patches (PP) mediate antigen entrance into the underlying lymphoid tissue. To investigate functional potential B this unique microcompartment, expression co-stimulatory molecules necessary for B-T cell interaction was examined histologically normal human PP by three-color immunohistochemistry. In areas, CD80 / CD86 much more frequent on memory (sIgD–CD20+) than naive (sIgD+CD20+) cells. areas identified such co-expression CD20 and were...
Fungal aerosols consist of spores and fragments with diverse array morphologies; however, the size, shape, origin constituents require further characterization. In this study, we characterize profile generated from Aspergillus fumigatus, A. versicolor, Penicillium chrysogenum grown for 8 weeks on gypsum boards. particles were aerosolized at 12 20 L min-1 using Spore Source Strength Tester (FSSST) Stami particle generator (SPG). Collected analyzed field emission scanning electron microscopy...
Association between selection pressure caused by the use of azole fungicides in sawmills and development fungal resistance has been described. The aim this study was to implement an algorithm assess presence Aspergillus section Fumigati resistant strains sawmills.Eighty-six full-shift inhalable dust samples were collected from eleven industrial Norway. Different culture media used molecular identification species level done calmodulin sequencing TR34/L98H TR46/Y121F/T289A mutations screened...
Occupational exposure during waste sorting is associated with several health outcomes. This study obtained knowledge about the impact of work in fully automated plants (AWSP; n = 3) vs manual (MWSP; on personal (n 71) to bioaerosols and exposure-related effects. Personal full-shift air samples were collected using various filter-based active sampling devices that placed workers' breathing zone. inhalable thoracic dust, endotoxin microorganisms varied considerably between within types (WSP)....
Employees at grain elevators and compound feed mills are exposed to large amounts of dust during work, frequently leading airway symptoms asthma. Although the exposure dust, microorganisms, β-1→3-glucans endotoxins has been extensively studied, focus on mycotoxin content previously limited one or few mycotoxins. Our objective was therefore screen settled from for fungal metabolites by LC/MS-MS explore differences between work places, seasons climatic zones. Seventy two bacterial were...
ABSTRACT Submicronic particles released from fungal cultures have been suggested to be additional sources of personal exposure in mold-contaminated buildings. In vitro generation these has studied with particle counters, eventually supplemented by autofluorescence, that recognize fragments size and discriminate biotic abiotic particles. However, the origin submicronic remains unclear. this study, derived Aspergillus fumigatus , A. versicolor Penicillium chrysogenum grown on agar gypsum board...
Sawmill workers are exposed to wood dust (a well-known carcinogen), microorganisms, endotoxins, resin acids (diterpenes), and vapours containing terpenes, which may cause skin irritation, allergy, respiratory symptoms including asthma. The health effects of most these exposures poorly understood as studies measure only dust. present study assessed in the Norwegian sawmill industry, processes predominantly spruce pine. Personal dust, acids, endotoxin, fungal spores fragments, mono-,...
Occupational exposure to bioaerosols during waste handling remains a health concern for exposed workers. However, exposure-related effects and underlying immunological mechanisms are still poorly described.The present study assessed the inflammatory potential of work-air samples (n = 56) in vitro investigated biomarker expression workers 69) compared unexposed controls 25). These quantitative results were self-reported conditions.Personal air provoked an activation TLR2 TLR4 HEK reporter...
This study assessed microorganisms in personal inhalable work air samples aiming to identify potential human pathogens, and correlate exposure adverse health outcomes waste workers. Full-shift was measured six different sorting plants. Microbial concentrations were analysed using MALDI-TOF MS for cultivable, next generation sequencing (NGS) non-cultivable microorganisms. Concentrations of bacterial fungal CFUs varied substantially within between plants, ranging from no identifiable organisms...
Mold particles from Aspergillus fumigatus, Penicillium chrysogenum, versicolor, and Stachybotrys chartarum have been linked to respiratory-related diseases. We characterized X-ray-inactivated spores hyphae fragments these species by number of particles, morphology, mycotoxin, β-glucan protease content/activity. The pro-inflammatory properties mold were examined in human bronchial epithelial cells (BEAS-2B) THP-1 monocytes phorbol 12-myristate 13-acetate (PMA)-differentiated THP-1. Spores P....
<h3>Objective</h3> We have studied cross-shift respiratory responses of several individual bioaerosol components the dust in grain and feed industry Norway. <h3>Methods</h3> Cross-shift changes lung function nasal congestion, as well systemic symptoms 56 exposed workers 36 referents, were recorded on same day full-shift exposure to inhalable aerosol fraction was assessed. Exposure–response associations investigated by regression analysis. <h3>Results</h3> The average 1.0 mg/m<sup>3</sup>...
The grain and compound feed industry entails inevitable risks of exposure to dust its microbial content. objective this study was therefore investigate task-dependent differences in order create knowledge basis for awareness reducing measures the Norwegian industry. A total 166 samples airborne were collected by full-shift personal sampling during work 20 elevators mills one autumn season two winter seasons. dust, endotoxins, β-1→3-glucans, bacteria, fungal spores quantified used as...
Submicronic fungal fragments have been observed in vitro aerosolization experiments. The occurrence of these particles has therefore suggested to contribute respiratory health problems mold-contaminated indoor environments. However, the role submicronic exacerbating adverse effects remained unclear due limitations associated with detection methods. In present study, we report development an indirect immunodetection assay that utilizes chicken polyclonal antibodies developed against spores...
Exposure to fungal spores has been associated with respiratory symptoms and allergic alveolitis among sawmill workers, but the complexity of workers' exposure poorly studied. We characterized diversity in air samples from breathing zones identified differences richness, diversity, taxonomic composition between companies, departments, wood types, seasons. Full-shift personal inhalable dust (n = 86) collected 11 industrial sawmill, sorting mill, planer mill companies processing spruce and/or...
Introduction It is of upmost importance to contribute fill the knowledge gap concerning characterization occupational exposure microbial agents in waste sorting setting (automated and manual sorting). Methods This study intends apply a comprehensive field sampling laboratory protocol (culture based-methods molecular tools), assess fungal azole resistance, as well elucidate on potential related health effects (cytotoxicity analyses). Skin-biota samples (eSwabs) were performed workers controls...
Assessment of exposure to fungi has commonly been limited fungal spore measurements that have shown associations between and development or exacerbation different airway diseases. Because large numbers submicronic fragments can be aerosolized from cultures under laboratory conditions, it suggested is more complex higher than revealed by measurements. However, the assessment in environmental matrix remain due methodological challenges. With a recently developed immunolabeling method for field...
The global shift toward greener societies demands new technologies and work operations in the waste-management sector. However, progressive industrial methods do not necessarily consider workers' health. This study characterized exposure to bioaerosols investigated bioaerosols' potential engage immune system vitro.Full personal aerosol sampling was conducted over three consecutive days. Dust load analyzed by gravimetry, fungal actinobacterial spores were scanning electron microscopy,...
This manuscript presents results on multifactorial characterization of bioaerosol exposure in Norwegian waste sorting plants and the potential such airborne dust to induce NF-κB reactions through TLR2 TLR4 activations an vitro reporter cell model system. Our data revealed that increasing fungal spore levels is associated with increased activation TLR4, whereas OTU richness decreasing these receptors.