A5081569692- Osteoarthritis Treatment and Mechanisms
- MicroRNA in disease regulation
- Spine and Intervertebral Disc Pathology
- Scoliosis diagnosis and treatment
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Mesenchymal stem cell research
- Fibroblast Growth Factor Research
- Connective tissue disorders research
- Pregnancy-related medical research
- Musculoskeletal pain and rehabilitation
- TGF-β signaling in diseases
- Cervical and Thoracic Myelopathy
- Endometriosis Research and Treatment
- Proteoglycans and glycosaminoglycans research
- Hippo pathway signaling and YAP/TAZ
- Circadian rhythm and melatonin
- Inflammatory mediators and NSAID effects
- Cell Adhesion Molecules Research
- Estrogen and related hormone effects
- Organ Transplantation Techniques and Outcomes
- Chemokine receptors and signaling
- Tendon Structure and Treatment
- Apelin-related biomedical research
- GDF15 and Related Biomarkers
Sun Yat-sen University
2015-2024
Third Affiliated Hospital of Sun Yat-sen University
2019-2024
National Medical Products Administration
2023
Key Laboratory of Guangdong Province
2019-2020
Sun Yat-sen Memorial Hospital
2015-2018
Hypertrophic differentiation is not only the terminal process of endochondral ossification in growth plate but also an important pathological change osteoarthritic cartilage. Collagen type II (COL2A1) was previously considered to be a structural component cartilage matrix, recently, it has been revealed extracellular signaling molecule that can significantly suppress chondrocyte hypertrophy. However, mechanisms by which COL2A1 regulates hypertrophic remain unclear. In our study, Col2a1...
Abstract Background Little is known about the implications of circRNAs in effects melatonin (MEL) on bone marrow mesenchymal stem cell (BMSC) osteogenic differentiation and osteoporosis (OP) progression. The aim our study was to investigate MEL-regulated BMSC OP Methods measured by qRT-PCR, western blot (WB), Alizarin Red, alkaline phosphatase (ALP) staining. Differential circRNA mRNA profiles BMSCs treated MEL were characterized deep sequencing, followed validation using RT-PCR, Sanger...
Osteoarthritis (OA) is a widespread arthritic disease and primary cause of disability. Increasing evidence suggests that inflammation has pivotal part in its pathogenesis. Interleukin-1β (IL-1β) mediator local inflammatory processes OA. Current therapies for OA mainly focus on the symptoms advanced stage disease. The possible utilization bone marrow mesenchymal stem cells (BMSCs) to regenerate cartilage an appealing method, but case requires chondrogenesis take place within inflamed...
Abstract Bone marrow‐derived mesenchymal stem cells ( BMSC s), with inherent chondrogenic differentiation potential appear to be ideally suited for therapeutic use in cartilage regeneration. Accumulating evidence has demonstrated that melatonin can promote human s. However, little is known about the mechanism. Micro RNA s (mi s) have been shown regulate of s, but their roles melatonin‐promoted not characterized. Here, we demonstrate promoted via upregulation miR‐526b‐3p and miR‐590‐5p....
Tumor necrosis factor-alpha (TNFα) plays a pivotal role in inflammation-related osteoporosis through the promotion of bone resorption and suppression formation. Numerous drugs have been produced to treat by inhibiting resorption, but they offer few benefits formation, which is what needed patients with severe loss. Melatonin, can exert both anti-inflammatory pro-osteogenic effects, shows promise overcoming TNFα-inhibited osteogenesis deserves further research. This study demonstrated that...
Accumulation of reactive oxygen species (ROS), which can be induced by inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α), significantly inhibit the osteogenic differentiation bone marrow mesenchymal stem cells (BMSCs). This process contribute to imbalance remodeling, ultimately leads osteoporosis. Therefore, reducing ROS generation during osteogenesis BMSCs may an effective way reverse impairment osteogenesis. Melatonin (MLT) has been reported act antioxidant cell...
Background: Intervertebral disc degeneration (IDD), the main cause of low back pain, is closely related to inflammatory microenvironment in nucleus pulposus (NP). Tumor necrosis factor-α (TNF-α) plays an important role inflammation-related metabolic disturbance NP cells. Melatonin has been proven regulate metabolism cells, but whether it can protect cells from TNF-α-induced damage still unclear. Therefore, this study aims investigate and specific mechanism melatonin on regulating...
The collagen type II alpha 1 (COL2A1) mutation causes severe skeletal malformations, but the pathogenic mechanisms of how this occurs are unclear. To understand may happen, a col2a1 p.Gly1170Ser mutated mouse model was constructed and in homozygotes, chondrodysplasia phenotype observed. Misfolded procollagen largely synthesized retained dilated endoplasmic reticulum stress (ERS)-unfolded protein response (UPR)-apoptosis cascade activated. Apoptosis occurred prior to hypertrophy, prevented...
Heparan sulfate (HS) interacts with growth factors and has been implicated in regulating chondrogenesis. However, the effect of HS on TGF‐ β ‐mediated mesenchymal stem cell (MSC) chondrogenesis molecular mechanisms remains unknown. In this study, we explored effects exogenous alone combination 3 chondrogenic differentiation human MSCs possible signal mechanisms. The results indicated that had no obvious /Smad2/3 pathways. combined 3/HS treatment resulted a significant increase GAG synthesis,...
Adolescent idiopathic scoliosis (AIS) is a complex genetic disorder characterized by three-dimensional spinal curvatures, affecting 2%-3% of school age children, yet the causes underlying AIS are not well understood. Here, we first conducted whole-exome sequencing and linkage analysis on three-generation Chinese family with autosomal-dominant (AD) AIS, then performed targeted in discovery cohort comprising 20 AD families 86 simplex patients, finally identified three disease-associated...
Degenerative disc disease (DDD) is a common degenerative condition initiated mainly within the nucleus pulposus (NP). To date, etiopathogenesis of DDD remains unclear, and because no effective therapeutic strategies are available to target its pathological processes, still treated with symptomatic interventions that far from adequate. Collagen type II one major matrix components NP, considered be essential NP homeostasis. However, specific mechanisms by which collagen influences cells remain...
Unbalanced metabolism of an extracellular matrix (ECM) in nucleus pulposus cells (NPCs) is widely acknowledged as the primary cause intervertebral disc degeneration (IDD). Irisin, a novel myokine, cleaved from fibronectin type III domain-containing 5 (FNDC5) and has recently been proven to regulate ECM. However, little known about its potential on NPCs development IDD. Therefore, this study sought examine protective effects molecular mechanism irisin IDD vivo vitro. Decreased expression...
This study aimed to verify the effects of estrogen on onset and development adolescent idiopathic scoliosis mechanisms associated with these by constructing a pubescent bipedal rat model. Experiments were conducted investigate whether progression was prevented Triptorelin treatment. One hundred twenty rats divided into female, OVX (ovariectomy), + E2, Triptorelin, sham, male groups. According spinal radiographic analysis, rates curve severity female E2 groups higher than those in OVX, The...
Introduction Degenerative lumbar spinal stenosis is a common cause of low back or leg pain and disability in the elderly population. Patients with who fail to respond conservative treatment often require surgical interventions. Minimally invasive transforaminal interbody fusion (TLIF) microscopic tubular technique (MT-TLIF) well-established procedure for stenosis. Recently, novel MIS technique, unilateral biportal endoscopic TLIF (UBE-TLIF), has been frequently performed treat However,...
Abstract Background: Little is known about the implications of circRNAs in effects Melatonin (MEL) on bone marrow mesenchymal stem cells (BMSCs) osteogenic differentiation and osteoporosis progression.The aim our study was to investigate MEL-regulated BMSCs progression. Methods: measured by qRT-PCR, western blot (WB), Alizarin Red alkaline phosphatase (ALP) staining. Differential circRNA mRNA profiles treated MEL were characterized deep sequencing, followed validation using RT-PCR, Sanger...
Abstract Background: To investigate circRNAs in Melatonin (MEL)-regulated bone marrow mesenchymal stem cell (BMSC) differentiation and osteoporosis. Methods: BMSC osteogenic was measured by qRT-PCR, western blot (WB), Alizarin Red alkaline phosphatase (ALP) staining. Differential circRNA mRNA profiles of BMSCs treated MEL were characterized deep sequencing, followed validation using RT-PCR, Sanger qRT-PCR. Silencing overexpression circ_0003865 conducted for functional investigations. The...